FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate6_96.3520000005f929.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate6_96.3520000005f929.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15662
Sequences flagged as poor quality0
Sequence length33
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCCACGCTTAACGTACTCTGCGTTGATACCA2801.787766568765164No Hit
GTGAATGGGTTCCACGCAAAAAAAAAAAAAAAA790.5044055676158856No Hit
TATCAACGCAGAGTGAATGGGTTCCACGCAAAA780.49802068701315283No Hit
GTTCCACGCAAAAAAAAAAAAAAAAAAAAAAAA770.4916358064104201No Hit
ACGCAGAGTGAATGGGTTCCACGCAAAAAAAAA630.402247477972162No Hit
GAGTGAATGGGTTCCACGCAAAAAAAAAAAAAA460.29370450772570555No Hit
GTGAATGGGTTCCACGCTAAAAAAAAAAAAAAA440.2809347465202401No Hit
AACGCAGAGTGAATGGGTTCCACGCAAAAAAAA410.2617801047120419No Hit
TATCAACGCAGAGTGAATGGGTTCCACGCTAAA380.24262546290384368No Hit
GTTCCACGCTAAAAAAAAAAAAAAAAAAAAAAA380.24262546290384368No Hit
GTATCAACGCAGAGTGAATGGGTTCCACGCAAA350.2234708210956455No Hit
GTGAATGGGGTTCCACGCAAAAAAAAAAAAAAA310.1979312986847146No Hit
ACGCAGAGTGAATGGGTTCCACGCTAAAAAAAA310.1979312986847146No Hit
GAGTGAATGGGTTCCACGCTAAAAAAAAAAAAA300.19154641808198186No Hit
GTGAATGGGGTTCCACAAAAAAAAAAAAAAAAA290.18516153747924913No Hit
CGCTTAACGTACTCAAAAAAAAAAAAAAAAAAA290.18516153747924913No Hit
GTGAATGGGTTCCACAAAAAAAAAAAAAAAAAA270.1723917762737837No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA260.16600689567105095No Hit
GTATCAACGCAGAGTGAATGGGTTCCACGCTAA260.16600689567105095No Hit
GTGAATGGGTTCCACGAAAAAAAAAAAAAAAAA250.15962201506831822No Hit
TTCCACGCTTAACGTACTCTGCGTTGATACCAC210.1340824926573873No Hit
GTGAATGGGGTTCCACGAAAAAAAAAAAAAAAA210.1340824926573873No Hit
TATCAACGCAGAGTGAATGGGTTCCACAAAAAA200.12769761205465457No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA190.12131273145192184No Hit
ATCAACGCAGAGTGAATGGGTTCCACGCAAAAA190.12131273145192184No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA170.10854297024645639No Hit
TATCAACGCAGAGTGAATGGGTTCCACGAAAAA160.10215808964372365No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT160.10215808964372365No Hit
GAGTGAATGGGGTTCCACGCAAAAAAAAAAAAA160.10215808964372365No Hit
TATCAACGCAGAGTGAATGGGTTCCACGCTTAA160.10215808964372365No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATACC701.5822297E-719.28571526
GTATCAA852.8503564E-919.0588231
GTTGATA753.2708704E-718.024
CGTTGAT753.2708704E-718.023
TTGATAC806.4384767E-716.87525
GATACCA756.7082237E-616.19999927
TATCAAC1151.89417E-612.9130442
GCGTTGA1305.310885E-712.46153827
ATCAACG1307.4754407E-611.4230773
TCAACGC1451.9898853E-611.1724134
TGCGTTG2201.9281288E-1011.04545527
CAACGCA1554.433923E-610.4516135
CTGCGTT2409.984433E-99.562526
AACGCAG1904.9696308E-58.5263176
TCTGCGT3404.8567017E-108.33823527
CGCAGAG2953.0864612E-88.2372882
GCAGAGT3004.109279E-88.0999993
ACGCAGA2902.1626147E-77.91379261
CAGAGTG2902.1626147E-77.91379264
AGAGTGA2902.1626147E-77.91379265
Produced by FastQC (version 0.11.2)