FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate6_94.3520000005f8e4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate6_94.3520000005f8e4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70931
Sequences flagged as poor quality0
Sequence length33
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTTGCAGTAACGTACTCTGCGTTGATACCAC2600.36655341106145406No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2250.31720968264933525No Hit
GTACGTTACTGCAAAAAAAAAAAAAAAAAAAAA1670.2354400755663955No Hit
CCTCCTTGCAGTAACGTACTCTGCGTTGATACC1420.2001945552720249No Hit
GCATTGGATGGATGCCCGGGCATTGAGAAGGAA1400.19737491364847526No Hit
GGGAGATACCGTCTGTGATCCATGGATCTCCGA1250.17622760147185293No Hit
CATCTAAGTAGCTAAAGGAAGGGAAATCAACCG1230.1734079598483033No Hit
GCCATGGGGAGATACCGTCTGTGATCCATGGAT1140.16071957254232988No Hit
CTCTTTGGACTTTGAAAACTTAGCGAACTGAAA1120.15789993091878024No Hit
GAGTACGTTACTGCAAAAAAAAAAAAAAAAAAA1090.15367046848345575No Hit
CGTTAGTAGCGGCGAGCGAGAGCGGATTTGGGA1050.14803118523635644No Hit
CTCCTTGCAGTAACGTACTCTGCGTTGATACCA960.13534279793038304No Hit
TGGCTAGTCTGCGCTCTTTGGACTTTGAAAACT750.10573656088311174No Hit
GCTAAAGGAAGGGAAATCAACCGAGACCCCGTT720.10150709844778728No Hit
CATCTAAGTAGCTAAAAAAAAAAAAAAAAAAAA710.10009727763601245No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACTCA307.0493144E-527.0091741
GAGTTTA200.00854273527.0091741
GTTTAAG200.00854273527.0091743
AGTTTAA200.00854273527.0091742
ACAGCCC200.0085722226.9901264
TTAAGCA200.0085722226.9901265
TTTAAGC200.0085722226.9901264
TAACCTC352.0179001E-423.1343965
TCAGAGT352.0179001E-423.1343965
CTCAGAG352.0179001E-423.1343964
TGAGAAG404.9524003E-420.2568824
GAGAAGG404.9524003E-420.2568825
CATTGAG404.9524003E-420.2568821
CCTGACA404.9524003E-420.256883
AACTCAG404.9524003E-420.256882
ACTCAGA404.9524003E-420.256883
ATTGAGA404.9524003E-420.2568822
GAAGGAA404.9524003E-420.2568827
TGCGTTG7450.020.12092827
ATCCTGA350.005472083619.2922691
Produced by FastQC (version 0.11.2)