FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate6_93.3520000005f8ca.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate6_93.3520000005f8ca.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8941
Sequences flagged as poor quality0
Sequence length33
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA520.5815904261268314No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA410.45856168213846327No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA410.45856168213846327No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA380.4250083883234538No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA350.39145509450844423No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA300.3355329381500951No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA280.3131640756067554No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT260.2907952130634157No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA250.2796107817917459No Hit
TGGCTAGTCTGCGCTCTTTGGACTTTGAAAACT220.24605748797673638No Hit
GTGAATGGGATAGCGTCAAAAAAAAAAAAAAAA180.20131976289005704No Hit
GTGAATGGGTAGCGTCGAAAAAAAAAAAAAAAA160.17895090034671737No Hit
CATCTAAGTAGCTAAAGGAAGGGAAATCAACCG150.16776646907504755No Hit
AGCGTCGAGATACGTACTCTGCGTTGATACCAC150.16776646907504755No Hit
GCTAAAGGAAGGGAAATCAACCGAGACCCCGTT150.16776646907504755No Hit
GTGAATGGGATAGCGTCGAGAAAAAAAAAAAAA150.16776646907504755No Hit
GTGAATGGGTAGCGTCAAAAAAAAAAAAAAAAA150.16776646907504755No Hit
TATCAACGCAGAGTGAATGGGTAGCGTCGAGAT150.16776646907504755No Hit
CGTTAGTAGCGGCGAGCGAGAGCGGATTTGGGA140.1565820378033777No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA140.1565820378033777No Hit
GTAGCGTCGAGATACGTACTCTGCGTTGATACC140.1565820378033777No Hit
ATCAACGCAGAGTGAATGGGAAGCAGTGGTATC130.14539760653170786No Hit
GTGAATGGGGTAGCGTCAAAAAAAAAAAAAAAA130.14539760653170786No Hit
GGGAGATACCGTCTGTGATCCATGGATCTCCGA130.14539760653170786No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCC120.13421317526003804No Hit
CTTTGGACTTTGAAAACTTAGCGAACTGAAACA120.13421317526003804No Hit
CGTCGAGATACGTACTCTGCGTTGATACCACTG110.12302874398836819No Hit
CTCTTTGGACTTTGAAAACTTAGCGAACTGAAA110.12302874398836819No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA110.12302874398836819No Hit
GTGAATGGGGTATCTCGACGCTAAAAAAAAAAA110.12302874398836819No Hit
GCATTGGATGGATGCCCGGGCATTGAGAAGGAA110.12302874398836819No Hit
ACGCAGAGTGAATGGGTAGCGTCGAAAAAAAAA100.11184431271669835No Hit
GCCATGGGGAGATACCGTCTGTGATCCATGGAT100.11184431271669835No Hit
TCCAAGCTCCGTGGCTAGTCTGCGCTCTTTGGA100.11184431271669835No Hit
ATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA90.10065988144502852No Hit
GTGAATGGGTAGCGTCGAGAAAAAAAAAAAAAA90.10065988144502852No Hit
GTGAATGGGATAGCGTCGAGATAAAAAAAAAAA90.10065988144502852No Hit
ACCGTATCCAAGCTCCGTGGCTAGTCTGCGCTC90.10065988144502852No Hit
GTGAATGGGGTAGCGTAAAAAAAAAAAAAAAAA90.10065988144502852No Hit
ACGCAGAGTGAATGGGTAGCGTCAAAAAAAAAA90.10065988144502852No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGTTG901.9517847E-615.027
CTGCGTT1005.8360565E-613.49999926
TCTGCGT1909.094947E-1212.78947427
TATCAAC750.00196304412.5999991
TCAACGC900.007683637610.53
ATCAACG900.007683637610.52
CAACGCA900.007683637610.54
CTAGCGT1503.1856085E-59.98
CTCTGCG2704.274625E-109.526
ACTCTGC2851.1459633E-99.025
CGAGATA3002.746674E-109.015
GAGATAC2851.1459633E-99.016
AGATACG2851.1459633E-99.017
TCGAGAT3053.765308E-108.85245914
GATACGT2901.5716068E-98.84482718
GTACTCT2952.1445885E-98.69491523
TACTCTG2952.1445885E-98.69491524
ATACGTA3002.908564E-98.54999919
ACGTACT2952.183151E-88.23728821
TACGTAC2952.183151E-88.23728820
Produced by FastQC (version 0.11.2)