FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate6_91.3520000005f887.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate6_91.3520000005f887.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43626
Sequences flagged as poor quality0
Sequence length33
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCCAGCTAGACGTACTCTGCGTTGATACCAC4230.9696052812542979No Hit
ACATCCAGCTAGACGTACTCTGCGTTGATACCA1820.41718241415669555No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1500.3438316600192546No Hit
GTGAATGGGACATCCAGCAAAAAAAAAAAAAAA1190.2727731169486086No Hit
ATCCAGCTAGACGTACTCTGCGTTGATACCACT880.2017145738779627No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA630.14440929720808693No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA620.1421170861412919No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT560.12836381974052172No Hit
GTGAATGGGACATCCAGCTAGAAAAAAAAAAAA560.12836381974052172No Hit
CCCACATCCAGCTAGACGTACTCTGCGTTGATA550.12607160867372666No Hit
CATCCAGCTAGACGTACTCTGCGTTTATACCAC510.11690276440654654No Hit
GTGAATGGGACATCCAGAAAAAAAAAAAAAAAA500.11461055333975154No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCC490.11231834227295649No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGC480.11002613120616146No Hit
TATCAACGCAGAGTGAATGGGACATCCAGCAAA470.10773392013936645No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA470.10773392013936645No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA460.1054417090725714No Hit
GTGAATGGGACATCCAAAAAAAAAAAAAAAAAA450.10314949800577637No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA450.10314949800577637No Hit
CCCCCACATCCAGCTAGACGTACTCTGCGTTGA450.10314949800577637No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCACAC200.00850895527.0080364
CCACACA200.00855681926.9770625
CACACAT300.002241155422.4808866
ATACCAC658.011739E-820.77541427
GATACCA701.7508319E-719.29145426
TGATACC757.2737803E-616.20482325
TTGATAC852.3798584E-514.29837224
TCTGCGT8600.012.87592427
CGCACAT750.00220604812.5892966
ACACATC1203.4724635E-612.3644887
GTTGATA1553.0529918E-812.19717927
GGGACAT1401.5125806E-611.56167
TATCAAC1308.3756895E-611.4264771
ATCAACG1401.905885E-510.61032
ATGGGAC1401.9303683E-510.5981335
CTACATC900.00860600810.491087
CTCTGCG10600.010.44650626
GGACATC1555.1330753E-610.4427358
GCACATC1301.0757004E-410.3757947
TGGGACA1452.8440469E-510.2326796
Produced by FastQC (version 0.11.2)