FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate6_90.3520000005f86d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate6_90.3520000005f86d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21244
Sequences flagged as poor quality0
Sequence length33
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGTACTCTGCGTTGATACAAAAAAAAAAAAA1150.5413293165128977No Hit
AACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA1110.5225004707211448No Hit
TATCAACGCAGAGTGAATGGGAACGTACTCTGC710.3342120128036151No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA700.3295048013556769No Hit
GTATCAACGCAGAGTGAATGGGAACGTACTCTG700.3295048013556769No Hit
ACTCTGCGTTGATACAAAAAAAAAAAAAAAAAA630.2965543212201092No Hit
AACGTACTCTGCGTTGATACCAAAAAAAAAAAA610.2871398983242327No Hit
TATCAACGCAGAGTGAATGGGAAACGTACTCTG600.28243268687629447No Hit
GCGTTGATACAAAAAAAAAAAAAAAAAAAAAAA560.26360384108454155No Hit
GTATCAACGCAGAGTGAATGGGAAACGTACTCT550.25889662963660326No Hit
GTGAATGGGAACGTACTCTGCGTTGATACAAAA540.25418941818866503No Hit
GAAACGTACTCTGCGTAAAAAAAAAAAAAAAAA520.24477499529278854No Hit
AACGTACTCTGCGTTGAAAAAAAAAAAAAAAAA460.2165317266051591No Hit
ACGCAGAGTGAATGGGAACGTACTCTGCGTTGA460.2165317266051591No Hit
GTGAATGGGCAGTCTCTGGAAAAAAAAAAAAAA430.2024100922613444No Hit
GTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA400.18828845791752966No Hit
AACGCAGAGTGAATGGGAACGTACTCTGCGTTG370.17416682357371494No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA350.16475240067783845No Hit
GTACTCTGCGTTGATACAAAAAAAAAAAAAAAA340.16004518922990021No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA340.16004518922990021No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA330.15533797778196196No Hit
GCGTTGATACCAAAAAAAAAAAAAAAAAAAAAA330.15533797778196196No Hit
GGAACGTACTCTGCGTTGATACAAAAAAAAAAA320.15063076633402372No Hit
GTCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA320.15063076633402372No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA310.1459235548860855No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT310.1459235548860855No Hit
TATCAACGCAGAGTGAATGGGCAGTCTCTGGAA300.14121634343814723No Hit
GTGAATGGGCAGTCTCAAAAAAAAAAAAAAAAA290.136509131990209No Hit
AACGTACTCTGCGTTAAAAAAAAAAAAAAAAAA280.13180192054227077No Hit
AACGTACTCTGCGAAAAAAAAAAAAAAAAAAAA270.12709470909433251No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA250.11768028619845604No Hit
GTGAATGGGAACGTACTCTGCAAAAAAAAAAAA250.11768028619845604No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA250.11768028619845604No Hit
GTGAATGGGAACGTACTCTGCGTTGATACCAAA240.1129730747505178No Hit
GAACGTACTCTGCGTTGATACAAAAAAAAAAAA240.1129730747505178No Hit
GTGAATGGGACAGTCTCTGGAAAAAAAAAAAAA240.1129730747505178No Hit
GGTATCAACGCAGAGTGAATGGGAAACGTACTC230.10826586330257955No Hit
ACTCTGCGTTGATACCAAAAAAAAAAAAAAAAA230.10826586330257955No Hit
ACGCAGAGTGAATGGGCAGTCTCTGGAAAAAAA230.10826586330257955No Hit
AACGTACTCTGCAAAAAAAAAAAAAAAAAAAAA220.1035586518546413No Hit
GTGAATGGGAAACGTACTCTGCGTAAAAAAAAA220.1035586518546413No Hit
GAGTGAATGGGAACGTACTCTGCGTTGATACAA220.1035586518546413No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAT257.579741E-426.9976431
CAACTCA200.0084280426.997641
AACTCAG200.0084280426.997642
ACTCAGA200.0084280426.997643
TCAGAGT200.0084280426.997645
CTCAGAG200.0084280426.997644
TATCATC300.002193777122.4980342
TCGCAGA300.002193777122.4980347
ATCATCG300.002193777122.4980343
TCATCGC300.002193777122.4980344
ATCGCAG350.005362063619.284036
GTATCAA954.033387E-614.2092841
TATCAAC1551.2369128E-1013.9342671
GCCAGTC600.007238976713.498827
ATCAGTC600.007238976713.498827
TCTGCGT7650.013.26542527
ATCAACG1707.530616E-911.9107252
TCAACGC1801.7575076E-811.2490183
ACCAGTC1256.774075E-510.7990567
CCAGTCT4700.010.3395218
Produced by FastQC (version 0.11.2)