FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate6_9.3520000005ee41.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate6_9.3520000005ee41.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20163
Sequences flagged as poor quality0
Sequence length33
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGTACCGTAACGTACTCTGCGTTGATACC2081.0315925209542232No Hit
GCATTGGATGGATGCCCGGGCATTGAGAAGGAA1520.7538560730050092No Hit
GGGAGATACCGTCTGTGATCCATGGATCTCCGA1220.6050686901750731No Hit
CATCTAAGTAGCTAAAGGAAGGGAAATCAACCG1010.5009175221941179No Hit
GCTACGTACCGTAACGTACTCTGCGTTGATACC1010.5009175221941179No Hit
CTACGTACCGTAACGTACTCTGCGTTGATACCA850.42156425135148545No Hit
GCCATGGGGAGATACCGTCTGTGATCCATGGAT810.40172593364082726No Hit
TGGCTAGTCTGCGCTCTTTGGACTTTGAAAACT770.3818876159301691No Hit
TCTACGTACCGTAACGTACTCTGCGTTGATACC720.3570897187918465No Hit
GCTACGTACCGTAAAAAAAAAAAAAAAAAAAAA690.34221098050885285No Hit
ACTACGTACCGTAACGTACTCTGCGTTGATACC630.31245350394286564No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT600.297574765659872No Hit
GCTAAAGGAAGGGAAATCAACCGAGACCCCGTT590.2926151862322075No Hit
CTCTTTGGACTTTGAAAACTTAGCGAACTGAAA580.287655606804543No Hit
CGTTAGTAGCGGCGAGCGAGAGCGGATTTGGGA540.2678172890938848No Hit
TCCAAGCTCCGTGGCTAGTCTGCGCTCTTTGGA540.2678172890938848No Hit
CTTTGGACTTTGAAAACTTAGCGAACTGAAACA540.2678172890938848No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA530.2628577096662203No Hit
TCTGTGATCCATGGATCTCCGATCGGGAAACCG460.22814065367256858No Hit
GCTACGTACCGAAAAAAAAAAAAAAAAAAAAAA450.22318107424490405No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA450.22318107424490405No Hit
AGTCTGCGCTCTTTGGACTTTGAAAACTTAGCG430.21326191538957495No Hit
CATCTAAGTAGCTAAAAAAAAAAAAAAAAAAAA420.20830233596191042No Hit
AGCTAAAGGAAGGGAAATCAACCGAGACCCCGT410.2033427565342459No Hit
TATCCAAGCTCCGTGGCTAGTCTGCGCTCTTTG390.19342359767891684No Hit
GTGAATGGGGAATGCATTGGATGGATGCCCGGG390.19342359767891684No Hit
GATTTGGGATTTTAAAAAAAAAAAAAAAAAAAA380.1884640182512523No Hit
GTGAATGGGCTACGTACCGAAAAAAAAAAAAAA370.18350443882358775No Hit
GGCGAAAGGCCATGGGGAGATACCGTCTGTGAT350.17358527996825868No Hit
CCCCTACGTACCGTAACGTACTCTGCGTTGATA340.16862570054059417No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA340.16862570054059417No Hit
ACCGTATCCAAGCTCCGTGGCTAGTCTGCGCTC340.16862570054059417No Hit
GGATGGATGCCCGGGCATTGAGAAGGAAGGACG320.15870654168526507No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA300.148787382829936No Hit
GGCATTGAGAAGGAAGGACGCTTTCAGAGGCGA300.148787382829936No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA300.148787382829936No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA300.148787382829936No Hit
GATACCGTCTGTGATCCATGGATCTCCGATCGG290.1438278034022715No Hit
CTCCGTGGCTAGTCTGCGCTCTTTGGACTTTGA290.1438278034022715No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA280.13886822397460696No Hit
CCCTACGTACCGTAACGTACTCTGCGTTGATAC270.1339086445469424No Hit
GTCTGCGCTCTTTGGACTTTGAAAACTTAGCGA270.1339086445469424No Hit
ACCGAGACCCCGTTAGTAGCGGCGAGCGAGAGC270.1339086445469424No Hit
CCGTATCCAAGCTCCGTGGCTAGTCTGCGCTCT260.1289490651192779No Hit
CCCCGTTAGTAGCGGCGAGCGAGAGCGGATTTG250.12398948569161336No Hit
TATCAACGCAGAGTGAATGGGCTACGTACCGAA250.12398948569161336No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA240.11902990626394881No Hit
GAGATACCGTCTGTGATCCATGGATCTCCGATC240.11902990626394881No Hit
GTGAATGGGCTACGTACAAAAAAAAAAAAAAAA240.11902990626394881No Hit
ACTACGTACCGAAAAAAAAAAAAAAAAAAAAAA240.11902990626394881No Hit
GCCCTACGTACCGTAACGTACTCTGCGTTGATA230.11407032683628429No Hit
CCTCTACGTACCGTAACGTACTCTGCGTTGATA230.11407032683628429No Hit
GTACGTAGCAAAAAAAAAAAAAAAAAAAAAAAA230.11407032683628429No Hit
TACCGTCTGTGATCCATGGATCTCCGATCGGGA230.11407032683628429No Hit
GTCTGTGATCCATGGATCTCCGATCGGGAAACC230.11407032683628429No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA220.10911074740861974No Hit
TATCAACGCAGAGTGAATGGGGAATGCATTGGA220.10911074740861974No Hit
ATGGATCTCCGATCGGGAAACCGTATCCAAGCT220.10911074740861974No Hit
CCTACGTACCGAAAAAAAAAAAAAAAAAAAAAA210.10415116798095521No Hit
GAGAAGGAAGGACGCTTTCAGAGGCGAAAGGCC210.10415116798095521No Hit
CATTGGATGGATGCCCGGGCATTGAGAAGGAAG210.10415116798095521No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCAT509.72113E-518.917
CATCTAA651.7637212E-618.6923081
CTAAGTA651.7637212E-618.6923084
TAGCTAA651.7637212E-618.6923089
TCTAAGT651.7637212E-618.6923083
CCCGGGC450.001059645718.015
TGCCCGG450.001059645718.013
CCGGGCA450.001059645718.016
GCCCGGG450.001059645718.014
AAGTAGC703.5814192E-617.3571436
AGTAGCT703.5814192E-617.3571437
TAAGTAG703.5814192E-617.3571435
ATCTAAG703.5814192E-617.3571432
AGCTAAA703.5814192E-617.35714310
GCTAAAG703.5814192E-617.35714311
GTAGCTA703.5814192E-617.3571438
GGGCATT552.0178694E-417.18181818
CATTGAG552.0178694E-417.18181821
ATTGAGA552.0178694E-417.18181822
GCATTGA552.0178694E-417.18181820
Produced by FastQC (version 0.11.2)