FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate6_87.3520000005f800.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate6_87.3520000005f800.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24004
Sequences flagged as poor quality0
Sequence length33
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCC3051.2706215630728213No Hit
GGCTAAATACGGGCGAGAGACCGATAGCGAACA960.39993334444259293No Hit
GCTAAATACGGGCGAGAGACCGATAGCGAACAA920.3832694550908182No Hit
GCCCCAATCGGGCGGTAAATTCCGTCCAAGGCT910.37910348275287453No Hit
GTTTGGGAATGCAGCCCCAATCGGGCGGTAAAT800.3332777870354941No Hit
AACAAGTACCGCGAGGTAAAAAAAAAAAAAAAA800.3332777870354941No Hit
GGTAAATTCCGTCCAAGGCTAAATACGGGCGAG790.3291118146975504No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGC730.3041159806698883No Hit
GTGAATGGGAGCTGACGAAAAAAAAAAAAAAAA680.28328611898017No Hit
CTGACGAAGTACGTACTCTGCGTTGATACCACT640.26662222962839527No Hit
GACCGATAGCGAACAAGTACCGCGAGGTAAAAA640.26662222962839527No Hit
TGCCCGGACCCTGTCGCACCACGAGGCGCTGTC630.2624562572904516No Hit
GGCGAGAGACCGATAGCGAACAAGTACCGCGAG600.24995834027662056No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA580.2416263956007332No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT580.2416263956007332No Hit
GGCCTAAGTTCCCTGGAAAGGGGCGCCAGAGAG580.2416263956007332No Hit
AAATACGGGCGAGAGACCGATAGCGAACAAGTA550.2291284785869022No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA550.2291284785869022No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA540.2249625062489585No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA500.2082986168971838No Hit
GCTGACGAAGTACGTACTCTGCGTTGATACCAC480.19996667222129647No Hit
GTCCAAGGCTAAATACGGGCGAGAGACCGATAG470.19580069988335275No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA460.1916347275454091No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA450.18746875520746542No Hit
CTGTCTACGAGTCGGGTTGTTTGGGAATGCAGC430.17913681053157807No Hit
CAAGTACCGCGAGGTAAAAAAAAAAAAAAAAAA400.16663889351774705No Hit
GCGCCAGAGAGGGTGAGAGCCCCGTCGTGCCCG400.16663889351774705No Hit
CTGTCGCACCACGAGGCGCTGTCTACGAGTCGG400.16663889351774705No Hit
ATGCAGCCCCAATCGGGCGGTAAATTCCGTCCA400.16663889351774705No Hit
CTACGAGTCGGGTTGTTTGGGAATGCAGCCCCA370.154140976503916No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA360.14997500416597234No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCT360.14997500416597234No Hit
AGGCTAAATACGGGCGAGAGACCGATAGCGAAC360.14997500416597234No Hit
GGTGAGAGCCCCGTCGTGCCCGGACCCTGTCGC350.14580903182802868No Hit
GTAAATTCCGTCCAAGGCTAAATACGGGCGAGA340.141643059490085No Hit
GTCGTGCCCGGACCCTGTCGCACCACGAGGCGC330.13747708715214133No Hit
CCCCAGGTCAGGCGGGATTACCCGCTGAGTTTA330.13747708715214133No Hit
GTTAAAAGATAACGCAGGTGTCCTAAGATGAGC320.13331111481419763No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA320.13331111481419763No Hit
GACCGATAGCGAACAAGTACCGCGAGGTAAAGA320.13331111481419763No Hit
ATACGGGCGAGAGACCGATAGCGAACAAGTACC320.13331111481419763No Hit
CTGGAGAAGCGTCCTCAGCGACGGACCGGGCCT320.13331111481419763No Hit
GTCTGGAGAAGCGTCCTCAGCGACGGACCGGGC320.13331111481419763No Hit
GTACGTACTCAAAAAAAAAAAAAAAAAAAAAAA310.12914514247625394No Hit
GTCCTAAGATGAGCTCAACGAGAACAGAAATCT310.12914514247625394No Hit
GTGTAGATCTCGGTGGTCGCCGTATCATTAAAA290.1208131978003666Illumina Single End PCR Primer 1 (100% over 29bp)
GTACGTACTCTGCGTAAAAAAAAAAAAAAAAAA290.1208131978003666No Hit
AGCTGACGAAGTACGTACTCTGCGTTGATACCA290.1208131978003666No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAA280.11664722546242293No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA280.11664722546242293No Hit
TGCTAACGCAATGTGATTTCTGCCCAGTGCTCT280.11664722546242293No Hit
GAGTGAATGGGAGCTGACGAAAAAAAAAAAAAA280.11664722546242293No Hit
GGGTGAGAGCCCCGTCGTGCCCGGACCCTGTCG280.11664722546242293No Hit
GGAGAAGCGTCCTCAGCGACGGACCGGGCCTAA270.11248125312447925No Hit
GTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAG270.11248125312447925No Hit
GAGGGTGAGAGCCCCGTCGTGCCCGGACCCTGT270.11248125312447925No Hit
TATCAACGCAGAGTGAATGGGAGCTGACGAAGT260.10831528078653557No Hit
GCGACGGACCGGGCCTAAGTTCCCTGGAAAGGG250.1041493084485919No Hit
GAATTGTAGTCTGGAGAAGCGTCCTCAGCGACG250.1041493084485919No Hit
GTACCGCGAGGTAAAAAAAAAAAAAAAAAAAAA250.1041493084485919No Hit
CCACGAGGCGCTGTCTACGAGTCGGGTTGTTTG250.1041493084485919No Hit
GTCGCACCACGAGGCGCTGTCTACGAGTCGGGT250.1041493084485919No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGTTG1950.018.72706827
CCAGCTG1004.1856583E-714.8465577
TTGGGAA657.284884E-414.5350919
TTTGGGA657.284884E-414.5350918
TATCAAC1551.2732926E-1013.9322521
CTGCGTT2550.013.79030326
ATGGGGT600.007282445713.49686817
GTTTGGG700.001276158613.49686817
TGTTTGG700.001276158613.49686816
GAATGCA700.001276158613.49686823
CGGGTTG600.007282445713.49686811
ACGAGTC700.001276158613.4968685
TCGGGTT600.007282445713.49686810
ATCAACG1653.45608E-1013.0878732
TCAACGC1705.5479177E-1012.7029353
TCTACGA750.00214595112.5970772
GAGTCGG750.00214595112.5970777
TACGAGT750.00214595112.5970774
CGAGTCG750.00214595112.5970776
GTCTACG750.00214595112.5970771
Produced by FastQC (version 0.11.2)