FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate6_84.3520000005f7a2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate6_84.3520000005f7a2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61454
Sequences flagged as poor quality0
Sequence length33
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCGTTCTGACGTACTCTGCGTTGATACCAC2770.45074364565365965No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCC1110.18062290493702607No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1040.16923227129234875No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGC910.14807823738080514No Hit
GTGAATGGGACTGTCGAAAAAAAAAAAAAAAAA740.12041526995801738No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA660.10739740293552902No Hit
CCACTGTCGTTCTGACGTACTCTGCGTTGATAC630.10251570280209586No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT630.10251570280209586No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCACT200.00854815627.05
CCCAACT300.002240469422.55
ACCACTG608.772022E-720.256
GAATGCA350.00547466319.28571523
ATACCAC350.00547466319.28571527
TCTGCGT12550.017.42629427
CGCACTG653.9689774E-516.6153856
CCAACTG751.3470919E-414.4000016
ATCAACG1508.731149E-1114.4000012
TTGATAC1151.405715E-714.08695627
TCAACGC1501.366061E-913.5000013
TATCAAC1704.3655746E-1113.51
CTCTGCG16300.013.41717826
TACTGTC3550.013.309868
CAACGCA1654.0017767E-1013.0909094
CTACTGT1552.2319E-913.0645167
ACTCTGC16850.012.8991125
GTATCAA1051.221797E-512.8571421
TGACTGT956.881911E-512.7894747
GTACTGT750.002209811212.67
Produced by FastQC (version 0.11.2)