FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate6_83.3520000005f788.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate6_83.3520000005f788.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25086
Sequences flagged as poor quality0
Sequence length33
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTTCTGTGACGTACTCTGCGTTGATACCAC2480.9885992186877142No Hit
GTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2260.9009009009009009No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA860.3428206968029977No Hit
GTCCTAAAAAAAAAAAAAAAAAAAAAAAAAAAA700.2790401020489516No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA550.21924579446703343No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA520.2072869329506498No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA510.2033006457785219No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA480.19134178426213824No Hit
GTGAATGGGGAGTCCAAAAAAAAAAAAAAAAAA460.1833692099178825No Hit
CAGTCCTTCTGTGACGTACTCTGCGTTGATACC430.17141034840149885No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA420.16742406122937098No Hit
GGGAGATACCGTCTGTGATCCATGGATCTCCGA420.16742406122937098No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT360.1435063381966037No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA340.13553376385234792No Hit
CCCCCAGTCCTTCTGTGACGTACTCTGCGTTGA340.13553376385234792No Hit
GTGAATGGGAGTCCTTCAAAAAAAAAAAAAAAA340.13553376385234792No Hit
CATCTAAGTAGCTAAAGGAAGGGAAATCAACCG330.13154747668022004No Hit
CGTTAGTAGCGGCGAGCGAGAGCGGATTTGGGA330.13154747668022004No Hit
GAGTCCTTCTGTGACGTACTCTGCGTTGATACC320.12756118950809214No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA300.1195886151638364No Hit
GTCCTTCTGTGACGTACTCTGCGTTTATACCAC290.11560232799170854No Hit
GTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA280.11161604081958065No Hit
CTCTTTGGACTTTGAAAACTTAGCGAACTGAAA270.10762975364745277No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA270.10762975364745277No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAT200.00845306227.01
TATCATC200.00845306227.02
CGTTGAT803.2943717E-818.562527
GCGTTGA2300.017.60869627
TATCAAC2550.013.7647071
CAAGTCC600.007283113413.54
TCAACGC2600.012.9807693
ATCAACG2600.012.9807692
GTATCAA1705.5842975E-1012.7058811
GGTATCA853.7485774E-412.7058811
CAACGCA2700.012.54
TCTGCGT7550.011.98013227
AACGCAG2900.011.1724145
TGCGTTG3800.011.01315826
ATGGGGC1000.001467807510.818
ACGCAGA3500.010.0285726
CTGCGTT4250.09.52941225
CTCTGCG9750.09.27692326
GAGTCCT2358.857751E-89.1914898
TCAGTCC1504.3352784E-49.08
Produced by FastQC (version 0.11.2)