FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate6_82.3520000005f76e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate6_82.3520000005f76e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23644
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGAGACGAGTACGTACTCTGCGTTGATACCA1930.8162747420064287No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCC910.3848756555574353No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGC750.3172052106242598No Hit
CATCGAGACGAGTACGTACTCTGCGTTGATACC520.21992894603282015No Hit
TATCAACGCAGAGTGAATGGGATCGAGACAAAA490.20724073760784978No Hit
GTGAATGGGATCGAGACAAAAAAAAAAAAAAAA440.18609372356623244No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA410.17340551514126204No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCT390.16494670952461513No Hit
GTTTGGGAATGCAGCCCCAATCGGGCGGTAAAT360.15225850109964473No Hit
GAGTGAATGGGATCGAGACAAAAAAAAAAAAAA360.15225850109964473No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA350.14802909829132127No Hit
GATCGAGACAAAAAAAAAAAAAAAAAAAAAAAA340.1437996954829978No Hit
GCCCCAATCGGGCGGTAAATTCCGTCCAAGGCT340.1437996954829978No Hit
GCTAAATACGGGCGAGAGACCGATAGCGAACAA300.12688208424970396No Hit
GAGTGAATGGGATCGAGACGAGAAAAAAAAAAA300.12688208424970396No Hit
GGCTAAATACGGGCGAGAGACCGATAGCGAACA300.12688208424970396No Hit
GCGCTTAAGCGCGCGACCTATACCCGGCCGTCG280.11842327863305702No Hit
ACGCAGAGTGAATGGGATCGAGACAAAAAAAAA270.11419387582473355No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA270.11419387582473355No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA260.10996447301641007No Hit
GGTAAATTCCGTCCAAGGCTAAATACGGGCGAG260.10996447301641007No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT260.10996447301641007No Hit
TATCAACGCAGAGTGAATGGGATCGAGACGAGA260.10996447301641007No Hit
TATCAACGCAGAGTGAATGGGACGAGTACAAAA250.10573507020808662No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCAAC751.2921129E-414.4000011
TCAACGC802.2357266E-413.53
AATGGGG700.001272950613.54
ATCAACG802.2357266E-413.52
CAACGCA906.046761E-412.04
TCTGCGT2759.822543E-119.81818327
AACGCAG1200.0066262219.05
TGCGTTG1651.1020726E-49.027
CATCGAG1802.8187344E-59.08
CTCTGCG3252.3392204E-98.30769326
CTGCGTT1802.8344858E-48.2526
GTGAATG2401.1188284E-57.8751
TGAATGG2401.1188284E-57.8752
CAGAGTG1550.00589854557.83870949
AGAGTGA1550.00589854557.838709410
CGCAGAG1550.00589854557.83870942
ACGCAGA1600.0078418147.593751
ACTCTGC3456.148548E-87.434782525
TACTCTG3507.92661E-87.32857224
GAATGGG2603.2508928E-57.2692313
Produced by FastQC (version 0.11.2)