FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate6_81.3520000005f745.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate6_81.3520000005f745.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48072
Sequences flagged as poor quality0
Sequence length33
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCC1630.33907472125145616No Hit
GCCATTCCGTTACGTACTCTGCGTTGATACCAC1190.24754534864370112No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1060.22050257946413712No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA980.2038608753536362No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA920.19137959727076054No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT920.19137959727076054No Hit
CCATTCCGTTACGTACTCTGCGTTGATACCACT900.18721917124313528No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA900.18721917124313528No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA890.1851389582293227No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA890.1851389582293227No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA770.1601764020635713No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA740.15393576302213346No Hit
GTTTGGGAATGCAGCCCCAATCGGGCGGTAAAT710.14769512398069562No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGC700.145614910966883No Hit
AGCCATTCCGTTACGTACTCTGCGTTGATACCA690.14353469795307042No Hit
GCTAAATACGGGCGAGAGACCGATAGCGAACAA680.14145448493925777No Hit
GAGCCATTCCGTTACGTACTCTGCGTTGATACC670.13937427192544516No Hit
CAGCCATTCCGTTACGTACTCTGCGTTGATACC660.13729405891163257No Hit
GGCTAAATACGGGCGAGAGACCGATAGCGAACA580.12065235480113164No Hit
GCCCCAATCGGGCGGTAAATTCCGTCCAAGGCT580.12065235480113164No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA570.11857214178731904No Hit
GTGAATGGGGAGTACGTAACGAAAAAAAAAAAA520.10817107671825595No Hit
GTACGTAACGAAAAAAAAAAAAAAAAAAAAAAA510.10609086370444334No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA500.10401065069063072No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTGA2600.019.73076827
TGCGTTG5300.013.75471727
TATCAAC2500.012.961
TCAGCCA906.253162E-412.05
ATCAACG2700.012.02
TCAACGC2850.011.3684213
CAACGCA3050.011.0655744
ATAGCCA1000.001511496510.7999997
CTGCGTT7100.010.457746526
AACGCAG3350.010.0746275
CTAGCCA1402.2653196E-49.6428577
TAGCCAT3800.09.5921058
TCTGCGT10750.09.41860527
ACGCAGA4301.8189894E-128.162796
CTCTGCG12500.08.126
GTATCAA2502.3895445E-68.11
CGCAGAG4403.6379788E-127.9772737
GCAGAGT4002.6011548E-107.76258
AAGCCAT2851.8599712E-67.5789476
ACTCTGC13450.07.52788125
Produced by FastQC (version 0.11.2)