FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate6_80.3520000005f71e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate6_80.3520000005f71e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42310
Sequences flagged as poor quality0
Sequence length33
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTGAATGGGCTAACTATCCAC4291.013944693925786No Hit
CTATCCACGTACTCAAAAAAAAAAAAAAAAAAA3710.8768612621129758No Hit
GTATCAACGCAGAGTGAATGGGCTAACTATCCA2660.6286929803828882No Hit
GTGAATGGGCTAACTATCCACAAAAAAAAAAAA2610.616875443157646No Hit
GGCTAACTATCCACGTACTCTGCGTTGATACCA2360.5577877570314347No Hit
ATCCACGTACTCAAAAAAAAAAAAAAAAAAAAA2010.4750649964547388No Hit
ACGCAGAGTGAATGGGCTAACTATCCACAAAAA1780.42070432521862444No Hit
TATCAACGCAGAGTGAATGGGGCTAACTATCCA1530.36161663909241315No Hit
ACGCAGAGTGAATGGGCTAACTATCCACGTACT1470.34743559442212246No Hit
GTGAATGGGCTAACTATCCACGTACTCAAAAAA1400.33089104230678323No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1330.3143464901914441No Hit
GGCTAACTATCCACAAAAAAAAAAAAAAAAAAA1220.2883479082959111No Hit
AACGCAGAGTGAATGGGCTAACTATCCACGTAC1130.2670763412904751No Hit
GAGTGAATGGGCTAACTATCCACAAAAAAAAAA1110.26234932640037817No Hit
GTGAATGGGGCTAACTATCCACGTACTCAAAAA1110.26234932640037817No Hit
GCTAACTATCCACGTACTCTGCGTTGATACCAC1070.25289529662018434No Hit
AACGCAGAGTGAATGGGCTAACTATCCACAAAA1020.2410777593949421No Hit
GTGAATGGGGCTAACTATCCACAAAAAAAAAAA980.23162372961474828No Hit
CTATCCACAAAAAAAAAAAAAAAAAAAAAAAAA970.22926022216969982No Hit
GTATCAACGCAGAGTGAATGGGGCTAACTATCC910.2150791774994091No Hit
GTGAATGGGCTAACTATCCAAAAAAAAAAAAAA910.2150791774994091No Hit
GGTATCAACGCAGAGTGAATGGGCTAACTATCC830.1961711179390215No Hit
ATCCACAAAAAAAAAAAAAAAAAAAAAAAAAAA790.1867170881588277No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCC770.1819900732687308No Hit
GGCTAACTATCCACGTACTCAAAAAAAAAAAAA740.17489955093358545No Hit
GAGTGAATGGGCTAACTATCCACGTACTCAAAA700.16544552115339162No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGC700.16544552115339162No Hit
ACGCAGAGTGAATGGGCTAACTATCCAAAAAAA660.15599149137319782No Hit
CGTACTCAAAAAAAAAAAAAAAAAAAAAAAAAA660.15599149137319782No Hit
ATCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA660.15599149137319782No Hit
GTGAATGGGCTAACTATCCACGTACAAAAAAAA660.15599149137319782No Hit
GAGTGAATGGGGCTAACTATCCACGTACTCAAA630.14890096903805247No Hit
GAGTGAATGGGGCTAACTATCCACAAAAAAAAA630.14890096903805247No Hit
CTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAA600.1418104467029071No Hit
ACTATCCACAAAAAAAAAAAAAAAAAAAAAAAA600.1418104467029071No Hit
CTATCCAAAAAAAAAAAAAAAAAAAAAAAAAAA590.13944693925785867No Hit
TATCAACGCAGAGTGAATGGGCTAACTATCCAA580.1370834318128102No Hit
GTATCATCGCAGAGTGAATGGGCTAACTATCCA570.13471992436776176No Hit
GTGAATGGGGCTAACTAAAAAAAAAAAAAAAAA570.13471992436776176No Hit
CTATCCACGTACAAAAAAAAAAAAAAAAAAAAA560.1323564169227133No Hit
ACTATCCACGTACTCAAAAAAAAAAAAAAAAAA540.1276294020326164No Hit
ATCAACGCAGAGTGAATGGGCTAACTATCCACG520.12290238714251948No Hit
ACGCAGAGTGAATGGGGCTAACTATCCACGTAC490.11581186480737414No Hit
GGGCTAACTATCCACGTACTCTGCGTTGATACC480.1134483573623257No Hit
ACGCAGAGTGAATGGGCTAACTATCCACGTACA470.11108484991727724No Hit
GTGAATGGGCTAACTAAAAAAAAAAAAAAAAAA460.10872134247222878No Hit
TATCTACGCAGAGTGAATGGGCTAACTATCCAC430.10163082013708344No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTACG356.3549796E-627.0177542
TCTACGC356.3549796E-627.0177543
CTACGAG200.00849121227.017753
TGTTTGG200.008540451526.98581516
GAGTCGG200.008540451526.9858157
TACGCAG405.8776095E-726.9858155
AGTCGGG200.008540451526.9858158
TACGAGT200.008540451526.9858154
CTACGCA405.8776095E-726.9858154
ACGCTGA300.002220477722.5147911
GTATCAT300.002220477722.5147911
TATCATC300.002220477722.5147912
CTGAGTG300.002235836322.4881784
TCATCGC300.002235836322.4881784
CATCGCA300.002235836322.4881785
TATCTAC454.4468165E-521.0138071
CGCTGAG350.005426287719.2983952
GCTGAGT350.005426287719.2983953
ATCATCG350.005426287719.2983953
TCGCAGA350.005463617419.2755857
Produced by FastQC (version 0.11.2)