FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate6_77.3520000005f6cc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate6_77.3520000005f6cc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22805
Sequences flagged as poor quality0
Sequence length33
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTTGGACCTTACGTACTCTGCGTTGATACCA3031.3286559964919973No Hit
GCTTGGACCTTACGTACTCTGCGTTGATACCAC2411.0567857925893445No Hit
GTGAATGGGAGGTCCAAAAAAAAAAAAAAAAAA1240.5437404078053059No Hit
GAGTACGTAAGGTCCAAAAAAAAAAAAAAAAAA1190.5218153913615435No Hit
GTACGTAAGGTCCAAAAAAAAAAAAAAAAAAAA1120.4911203683402763No Hit
GCCTTACGTACTCAAAAAAAAAAAAAAAAAAAA1000.43850032887524665No Hit
TATCAACGCAGAGTGAATGGGCCTTACGTACTC860.37711028283271214No Hit
GTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA730.32010524007893004No Hit
GTGAATGGGGGTCCAAAAAAAAAAAAAAAAAAA640.28064021048015786No Hit
GTGAATGGGTACGTAAGGTCCAAAAAAAAAAAA630.2762552071914054No Hit
GACCTTACGTACTCAAAAAAAAAAAAAAAAAAA600.263100197325148No Hit
GTGAATGGGGAGTACGTAAGGTCCAAAAAAAAA590.25871519403639553No Hit
TACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA550.24117518088138568No Hit
GTATCAACGCAGAGTGAATGGGCCTTACGTACT520.22802017101512828No Hit
GCTTGGACCAAAAAAAAAAAAAAAAAAAAAAAA490.2148651611488709No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA490.2148651611488709No Hit
GTGAATGGGCCTTACGTACTCAAAAAAAAAAAA490.2148651611488709No Hit
GTGAATGGGGTCCAAAAAAAAAAAAAAAAAAAA460.20171015128261346No Hit
GGTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA450.19732514799386097No Hit
GAGTGAATGGGGTCCAAAAAAAAAAAAAAAAAA400.17540013155009868No Hit
ACGCAGAGTGAATGGGCCTTACGTACTCAAAAA400.17540013155009868No Hit
TGCTTGGACCAAAAAAAAAAAAAAAAAAAAAAA390.17101512826134618No Hit
TATCAACGCAGAGTGAATGGGGTCCAAAAAAAA390.17101512826134618No Hit
TATCAACGCAGAGTGAATGGGACCTTACGTACT380.16663012497259372No Hit
GTGAATGGGAGTACGTAAGGTCCAAAAAAAAAA380.16663012497259372No Hit
ACCTTACGTACTCAAAAAAAAAAAAAAAAAAAA370.16224512168384128No Hit
GACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA350.15347511510633632No Hit
GTGAATGGGACCTTACGTACTCAAAAAAAAAAA340.14909011181758386No Hit
TATCAACGCAGAGTGAATGGGGGTCCAAAAAAA340.14909011181758386No Hit
GGCCTTACGTACTCAAAAAAAAAAAAAAAAAAA330.1447051085288314No Hit
GTGAATGGGGTACGTAAGGTCCAAAAAAAAAAA330.1447051085288314No Hit
CCTTACGTACTCAAAAAAAAAAAAAAAAAAAAA330.1447051085288314No Hit
GTGAATGGGTGCTTGGAAAAAAAAAAAAAAAAA320.14032010524007893No Hit
GGTATCAACGCAGAGTGAATGGGCCTTACGTAC320.14032010524007893No Hit
TATCAACGCAGAGTGAATGGGAGGTCCAAAAAA310.13593510195132646No Hit
TACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA310.13593510195132646No Hit
GGGTACGTAAGGTCCAAAAAAAAAAAAAAAAAA310.13593510195132646No Hit
GTGAATGGGTGCTTGGACCAAAAAAAAAAAAAA310.13593510195132646No Hit
ACGCAGAGTGAATGGGAGGTCCAAAAAAAAAAA300.131550098662574No Hit
GAGTGAATGGGGGTCCAAAAAAAAAAAAAAAAA300.131550098662574No Hit
GTGAATGGGGGTCCAAGCAAAAAAAAAAAAAAA290.12716509537382154No Hit
GTGCTTGGACCTTACGTACTCTGCGTTGATACC280.12278009208506907No Hit
GCTTGGACCTAAAAAAAAAAAAAAAAAAAAAAA280.12278009208506907No Hit
TACGTACTCTGCGTTGATACAAAAAAAAAAAAA280.12278009208506907No Hit
GTGAATGGGATGCTTGGACCTAAAAAAAAAAAA280.12278009208506907No Hit
GCTTGGACCTTACGTACTCTGCGTTTATACCAC270.11839508879631659No Hit
GAATGGGACCTTACGTACTCAAAAAAAAAAAAA270.11839508879631659No Hit
GAATGGGCCTTACGTACTCAAAAAAAAAAAAAA270.11839508879631659No Hit
ACGCAGAGTGAATGGGGTCCAAAAAAAAAAAAA270.11839508879631659No Hit
GTATCAACGCAGAGTGAATGGGACCTTACGTAC270.11839508879631659No Hit
AACGCAGAGTGAATGGGCCTTACGTACTCAAAA270.11839508879631659No Hit
GGTCCAAGCAAAAAAAAAAAAAAAAAAAAAAAA260.11401008550756414No Hit
GAGTGAATGGGGAGTACGTAAGGTCCAAAAAAA260.11401008550756414No Hit
CGTAAGGTCCAAAAAAAAAAAAAAAAAAAAAAA250.10962508221881166No Hit
GACCTAAAAAAAAAAAAAAAAAAAAAAAAAAAA250.10962508221881166No Hit
GTATCAACGCAGAGTGAATGGGGTCCAAAAAAA240.10524007893005918No Hit
ACGCAGAGTGAATGGGCCTTACGTACTCTGCGT240.10524007893005918No Hit
ACGTAAGGTCCAAAAAAAAAAAAAAAAAAAAAA230.10085507564130673No Hit
CGTTAGTAGCGGCGAGCGAGAGCGGATTTGGGA230.10085507564130673No Hit
AACGCAGAGTGAATGGGAGGTCCAAAAAAAAAA230.10085507564130673No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA1500.018.91
TCTGCGT5250.012.34285627
TATCAAC2400.011.8124992
ATCAACG2551.8189894E-1211.1176463
TCAACGC2451.0913936E-1111.0204094
TGGGGTC900.00834152410.513
CAACGCA2707.4760464E-109.54
CTCTGCG6950.09.32374126
CCTGCTT1853.8733495E-58.7567587
AACGCAG3151.2805685E-98.5714286
ATGCTTG2101.8306357E-58.3571428
ACTCTGC7950.08.32075525
ATGGGGT1500.00437839148.09999912
CTGCGTT2603.7461014E-67.78846127
GTACTCT8500.07.78235323
TACTCTG8600.07.691860724
TTGCTTG1600.0078198057.593758
TGGACCT8650.07.491329713
GACCTTA8650.07.491329715
GGACCTT8700.07.448275614
Produced by FastQC (version 0.11.2)