FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate6_76.3520000005f6a3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate6_76.3520000005f6a3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35045
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTCCTCTACGTACTCTGCGTTGATACCA3651.0415180482237123No Hit
CTCGTCCTCTACGTACTCTGCGTTGATACCACT1770.5050649165358825No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA750.2140105578541875No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA710.20259666143529748No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT700.19974318733057497No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCC690.19688971322585247No Hit
CATCTCGTCCTCTACGTACTCTGCGTTGATACC690.19688971322585247No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA630.17976886859751748No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA620.17691539449279498No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA600.17120844628334997No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA600.17120844628334997No Hit
GTGAATGGGATCTCGTCCAAAAAAAAAAAAAAA560.15979454986445998No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA530.1512341275502925No Hit
TATCAACGCAGAGTGAATGGGATCTCGTCCAAA490.1398202311314025No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA490.1398202311314025No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGC490.1398202311314025No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA410.11699243829362248No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCT380.108432015979455No Hit
GTATCAACGCAGAGTGAATGGGATCTCGTCCTC360.10272506777000998No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTGGG200.00851637326.98857117
GGGTTGT200.00851637326.98857112
CGGGTTG200.00851637326.98857111
CGAGTCG200.00851637326.9885716
GTCGGGT200.00851637326.9885719
TCGGGTT200.00851637326.98857110
GATACCA552.0566964E-417.19911627
TGATACC550.004105153514.74209926
TATCAAC1701.8189894E-1214.2880681
GCGTTGA1551.364242E-1013.94951327
ATCAACG1902.0008883E-1112.784062
TCAACGC1953.092282E-1112.4562643
CAACGCA2101.10958354E-1011.5665314
TCTGCGT5600.011.341763527
GCATCTC850.005571756511.1129427
CTCTGCG6500.09.77136626
AACGCAG2502.3301254E-99.7158855
ACTCTGC6650.09.55095925
TATCTCG1150.0047804799.3873298
TACTCTG6800.09.34027724
Produced by FastQC (version 0.11.2)