FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate6_75.3520000005f689.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate6_75.3520000005f689.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15964
Sequences flagged as poor quality0
Sequence length33
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACC690.43222250062640943No Hit
GTAGGATAGTGGCCTACCATGGTGGTAACGGGT630.3946379353545477No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA610.3821097469305938No Hit
ATCCTGACACGGGGAGGTAGTGACAAAAAAAAA590.36958155850663993No Hit
ACCATGGTGGTAACGGGTGACGGAGAATTAGGG530.33199699323477827No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA530.33199699323477827No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA520.32573289902280134No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA520.32573289902280134No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT480.3006765221748935No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCA480.3006765221748935No Hit
GAATTAGGGTTCGATTCCGGAGAGGGAGCCTGA430.26935605111500877No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA430.26935605111500877No Hit
CTACCATGGTGGTAACGGGTGACGGAGAATTAG420.26309195690303183No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA410.2568278626910549No Hit
GGCCTACCATGGTGGTAACGGGTGACGGAGAAT400.25056376847907796No Hit
GGATAGTGGCCTACCATGGTGGTAACGGGTGAC360.22550739163117012No Hit
CGGCTACCACATCCAAGGAAGGCAGCAGGCGCG360.22550739163117012No Hit
GGGAGGTAGTGACAAAAAAAAAAAAAAAAAAAA360.22550739163117012No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA350.21924329741919318No Hit
TGGTAGGATAGTGGCCTACCATGGTGGTAACGG350.21924329741919318No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA340.21297920320721622No Hit
TCGTTGGTCGAAACGTACTCTGCGTTGATACCA330.2067151089952393No Hit
CCATGGTGGTAACGGGTGACGGAGAATTAGGGT330.2067151089952393No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA330.2067151089952393No Hit
CAAATTACCCAATCCTGACACGGGGAGGTAGTG330.2067151089952393No Hit
ACCCAATCCTGACACGGGGAGGTAGTGACAAAA310.1941869205712854No Hit
GGTAACGGGTGACGGAGAATTAGGGTTCGATTC270.1691305437233776No Hit
GTGGTAACGGGTGACGGAGAATTAGGGTTCGAT270.1691305437233776No Hit
GGCAGCAGGCGCGCAAATTACCCAATCCTGACA260.16286644951140067No Hit
GAGAAACGGCTACCACATCCAAGGAAGGCAGCA240.15033826108744675No Hit
CCGGAGAGGGAGCCTGAGAAACGGCTACCACAT230.1440741668754698No Hit
AAGGAAGGCAGCAGGCGCGCAAATTACCCAATC220.13781007266349285No Hit
GAGTACGTTTCGACCAAAAAAAAAAAAAAAAAA220.13781007266349285No Hit
GTGAATGGGTCGTTGGTCAAAAAAAAAAAAAAA220.13781007266349285No Hit
TATCAACGCAGAGTGAATGGGGTCGTTGGAAAA210.13154597845151592No Hit
ATCCTGACACGGGGAGGTAGTGACAATAAAAAA210.13154597845151592No Hit
GATAGTGGCCTACCATGGTGGTAACGGGTGACG200.12528188423953898No Hit
CCCCAGGTCAGGCGGGATTACCCGCTGAGTTTA200.12528188423953898No Hit
ATCCTGACACGGGGAGGTAGTGAAAAAAAAAAA200.12528188423953898No Hit
GGTAGTGACAAAAAAAAAAAAAAAAAAAAAAAA200.12528188423953898No Hit
CTCGTTGGTCGAAACGTACTCTGCGTTGATACC200.12528188423953898No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA180.11275369581558506No Hit
AAATTACCCAATCCTGACACGGGGAGGTAGTGA180.11275369581558506No Hit
ACTTTCGATGGTAGGATAGTGGCCTACCATGGT170.10648960160360811No Hit
ATTCAAATTTCTGCCCTATCAACTTTCGATGGT170.10648960160360811No Hit
ATCCAAGGAAGGCAGCAGGCGCGCAAATTACCC170.10648960160360811No Hit
GTCGTTGGTCGAAACGTACTCTGCGTTGATACC170.10648960160360811No Hit
TTTCTGCCCTATCAACTTTCGATGGTAGGATAG170.10648960160360811No Hit
GGTGGTAACGGGTGACGGAGAATTAGGGTTCGA160.10022550739163118No Hit
GGTAGTGACAATAAAAAAAAAAAAAAAAAAAAA160.10022550739163118No Hit
GTGAATGGGTCGTTGGTCGAAAAAAAAAAAAAA160.10022550739163118No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGTTG802.9676812E-818.61871127
ACTCGTT450.001047204217.9979117
TCTGCGT2750.015.75666127
CTGCGTT1151.9044692E-612.91154426
ATCAACG1305.344227E-712.4600922
CTCTGCG3600.012.37356426
TCAACGC1358.4461317E-711.9986073
CAACGCA1358.4461317E-711.9986074
ACTCTGC3950.011.61890425
TATCAAC1401.3112422E-611.5700851
AACGCAG1452.002178E-611.1711165
TACTCTG4200.010.92730224
GTACTCT4250.010.79874623
CTCGTTG1802.0437983E-710.4987818
CGTACTC4500.010.19881522
AATGGGG1503.670503E-59.8988516
TTGGTCG4650.09.86982212
TGGTCGA4800.09.84260713
GGTCGAA4800.09.84260714
ACGTACT4600.09.68365921
Produced by FastQC (version 0.11.2)