FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate6_74.3520000005f66f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate6_74.3520000005f66f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35527
Sequences flagged as poor quality0
Sequence length33
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACACAGACAGTACGTACTCTGCGTTGATACCA6031.6973006445801784No Hit
AGACAGTACGTACTCTGCGTTGATACCACTGCT4181.1765699327272217No Hit
GACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2590.7290229965941397No Hit
GTGAATGGGACACAGACAAAAAAAAAAAAAAAA2350.6614687420834857No Hit
GTGAATGGGACACAGACAGAAAAAAAAAAAAAA2030.5713964027359474No Hit
GACACAGACAGAAAAAAAAAAAAAAAAAAAAAA1830.5151011906437357No Hit
GACACAGACAGTACAAAAAAAAAAAAAAAAAAA1700.4785093027837983No Hit
ACGCAGAGTGAATGGGACACAGACAAAAAAAAA1440.4053255270639232No Hit
TATCAACGCAGAGTGAATGGGACACAGACAAAA1430.4025107664593126No Hit
TATCAACGCAGAGTGAATGGGACACAGACAGTA1330.37436316041320683No Hit
GACACAGACAAAAAAAAAAAAAAAAAAAAAAAA1140.3208827089256059No Hit
GACAGTACGTACTCAAAAAAAAAAAAAAAAAAA1110.31243842711177416No Hit
GTACGTACTCAAAAAAAAAAAAAAAAAAAAAAA980.2758465392518366No Hit
TATCAACGCAGAGTGAATGGGACACAGACAGAA950.2674022574380049No Hit
ACGCAGAGTGAATGGGACACAGACAGAAAAAAA940.26458749683339433No Hit
GAGTGAATGGGACACAGACAAAAAAAAAAAAAA940.26458749683339433No Hit
GACACAGACAGTAAAAAAAAAAAAAAAAAAAAA910.25614321501956255No Hit
GTATCAACGCAGAGTGAATGGGACACAGACAAA880.24769893320573086No Hit
GTATCAACGCAGAGTGAATGGGACACAGACAGT810.2279956089734568No Hit
GAGTGAATGGGACACAGACAGAAAAAAAAAAAA800.22518084836884622No Hit
GACAGAAAAAAAAAAAAAAAAAAAAAAAAAAAA800.22518084836884622No Hit
GTGAATGGGGACACAGACAGAAAAAAAAAAAAA780.21955132715962508No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA750.21110704534579336No Hit
GTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA740.20829228474118275No Hit
GTGAATGGGGACACAGACAAAAAAAAAAAAAAA730.20547752413657216No Hit
GTGAATGGGACACAGACAGTAAAAAAAAAAAAA720.2026627635319616No Hit
GAATGGGACACAGACAAAAAAAAAAAAAAAAAA720.2026627635319616No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGC720.2026627635319616No Hit
GACACAGACAGTACGAAAAAAAAAAAAAAAAAA690.1942184817181299No Hit
AACGCAGAGTGAATGGGACACAGACAAAAAAAA680.1914037211135193No Hit
GTATCATCGCAGAGTGAATGGGACACAGACAAA590.1660708756720241No Hit
GACACAGACAGTACGTACTCAAAAAAAAAAAAA570.16044135446280294No Hit
GTGAATGGGACACAGACAGTACAAAAAAAAAAA560.15762659385819236No Hit
GAATGGGACACAGACAGAAAAAAAAAAAAAAAA550.1548118332535818No Hit
GTATCAACGCAGAGTGAATGGGACACAGACAGA540.1519970726489712No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCT540.1519970726489712No Hit
GGACACAGACAGTACGTACTCTGCGTTGATACC510.14355279083513947No Hit
AACGCAGAGTGAATGGGACACAGACAGAAAAAA510.14355279083513947No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCC480.13510850902130775No Hit
CAGTACGTACTCAAAAAAAAAAAAAAAAAAAAA480.13510850902130775No Hit
CAACTCAGAGTGAATGGGACACAGACAAAAAAA430.12103470599825485No Hit
AGACAGTACAAAAAAAAAAAAAAAAAAAAAAAA420.11821994539364428No Hit
GTGAATGGGGACACAGAAAAAAAAAAAAAAAAA420.11821994539364428No Hit
ACAGTACGTACTCTGCGTTGATACCACTGCTTC410.11540518478903368No Hit
ACACAGACAGTACGTACTCTGCGTTGATACCAC400.11259042418442311No Hit
GTGAATGGGACACAGAAAAAAAAAAAAAAAAAA400.11259042418442311No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA390.10977566357981254No Hit
GACAGTAAAAAAAAAAAAAAAAAAAAAAAAAAA380.10696090297520197No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA380.10696090297520197No Hit
GAGTGAATGGGGACACAGACAAAAAAAAAAAAA370.10414614237059137No Hit
GAATGGGGACACAGACAAAAAAAAAAAAAAAAA370.10414614237059137No Hit
GTATCATCGCAGAGTGAATGGGACACAGACAGA370.10414614237059137No Hit
TATCTACGCAGAGTGAATGGGACACAGACAAAA370.10414614237059137No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAT257.6502503E-427.0169281
TATCATC257.6502503E-427.0169282
ATCATCG257.6502503E-427.0169283
GTATTAA200.008474315527.0169241
GAGTAAA200.008474315527.0169241
ATTAACG200.008474315527.0169243
AGTAAAT200.008474315527.0169242
TATTAAC200.008474315527.0169242
TCGCAGA257.713731E-426.9788747
ATCGCAG257.713731E-426.9788746
TCATCGC257.713731E-426.9788744
TTAACGC200.00853293726.9788744
CATCGCA257.713731E-426.9788745
TATCTAC351.976946E-423.1573661
TACGCAG351.9959948E-423.124755
ACCACTG300.002213848822.51410525
ATCTACG300.002213848822.5141052
CACTGCT300.002213848822.51410527
CGTAGAG300.002213848822.5141053
TCTACGC300.002213848822.5141053
Produced by FastQC (version 0.11.2)