FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate6_72.3520000005f61f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate6_72.3520000005f61f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23465
Sequences flagged as poor quality0
Sequence length33
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACACTACACACGTACTCTGCGTTGATACCAC2551.0867249094395908No Hit
CCTCCACACTACACACGTACTCTGCGTTGATAC2260.9631365864052844No Hit
CATCTAAGTAGCTAAAGGAAGGGAAATCAACCG2090.8906882591093117No Hit
GGGAGATACCGTCTGTGATCCATGGATCTCCGA1790.7628382697634775No Hit
GCATTGGATGGATGCCCGGGCATTGAGAAGGAA1780.7585766034519498No Hit
CTCTTTGGACTTTGAAAACTTAGCGAACTGAAA1440.6136799488600042No Hit
CGTTAGTAGCGGCGAGCGAGAGCGGATTTGGGA1400.596633283613893No Hit
TGGCTAGTCTGCGCTCTTTGGACTTTGAAAACT1270.5412316215640315No Hit
GCCATGGGGAGATACCGTCTGTGATCCATGGAT1140.4858299595141701No Hit
GCTAAAGGAAGGGAAATCAACCGAGACCCCGTT1060.4517366290219476No Hit
TCCAAGCTCCGTGGCTAGTCTGCGCTCTTTGGA1030.43895163008736415No Hit
CTTTGGACTTTGAAAACTTAGCGAACTGAAACA980.4176432985297251No Hit
ACTCCACACTACACACGTACTCTGCGTTGATAC840.3579799701683358No Hit
CATCCACACTACACACGTACTCTGCGTTGATAC760.32388663967611336No Hit
ACCGTATCCAAGCTCCGTGGCTAGTCTGCGCTC760.32388663967611336No Hit
CTTCCACACTACACACGTACTCTGCGTTGATAC750.31962497336458556No Hit
CTCCACACTACACACGTACTCTGCGTTGATACC730.3111016407415299No Hit
CATCTAAGTAGCTAAAAAAAAAAAAAAAAAAAA710.3025783081184743No Hit
AGCTAAAGGAAGGGAAATCAACCGAGACCCCGT690.2940549754954187No Hit
TATCCAAGCTCCGTGGCTAGTCTGCGCTCTTTG660.2812699765608353No Hit
GCTCCACACTACACACGTACTCTGCGTTGATAC660.2812699765608353No Hit
TCTCCACACTACACACGTACTCTGCGTTGATAC650.2770083102493075No Hit
TCTGTGATCCATGGATCTCCGATCGGGAAACCG630.26848497762625184No Hit
CCCCGTTAGTAGCGGCGAGCGAGAGCGGATTTG590.25143831238014064No Hit
GTCCACACTACACACGTACTCTGCGTTGATACC580.24717664606861284No Hit
GTCTGCGCTCTTTGGACTTTGAAAACTTAGCGA570.24291497975708504No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA560.23865331344555724No Hit
GGATGGATGCCCGGGCATTGAGAAGGAAGGACG560.23865331344555724No Hit
ACCGAGACCCCGTTAGTAGCGGCGAGCGAGAGC550.2343916471340294No Hit
AGTCTGCGCTCTTTGGACTTTGAAAACTTAGCG540.2301299808225016No Hit
GAGATACCGTCTGTGATCCATGGATCTCCGATC540.2301299808225016No Hit
GAAATCAACCGAGACCCCGTTAGTAGCGGCGAG530.2258683145109738No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT530.2258683145109738No Hit
GATTTGGGATTTTAAAAAAAAAAAAAAAAAAAA520.221606648199446No Hit
GATACCGTCTGTGATCCATGGATCTCCGATCGG520.221606648199446No Hit
CGTGTGTAGTGTGGAAAAAAAAAAAAAAAAAAA510.2173449818879182No Hit
GTGAATGGGGAATGCATTGGATGGATGCCCGGG480.20455998295333475No Hit
TAGTAGCGGCGAGCGAGAGCGGATTTGGGATTT470.20029831664180692No Hit
CTTTGAAAACTTAGCGAACTGAAACATCTAAGT470.20029831664180692No Hit
CGTCCACACTACACACGTACTCTGCGTTGATAC460.19603665033027912No Hit
GTGTAGTGTGGAAAAAAAAAAAAAAAAAAAAAA450.19177498401875134No Hit
GGCATTGAGAAGGAAGGACGCTTTCAGAGGCGA440.18751331770722354No Hit
CTCCGTGGCTAGTCTGCGCTCTTTGGACTTTGA440.18751331770722354No Hit
GGCCATGGGGAGATACCGTCTGTGATCCATGGA430.1832516513956957No Hit
CGTGTGTAGTGTGAAAAAAAAAAAAAAAAAAAA430.1832516513956957No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA410.1747283187726401No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA410.1747283187726401No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA400.17046665246111228No Hit
GGCGAAAGGCCATGGGGAGATACCGTCTGTGAT370.15768165352652885No Hit
CTACACACGTACTCAAAAAAAAAAAAAAAAAAA370.15768165352652885No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA370.15768165352652885No Hit
CCCGTTAGTAGCGGCGAGCGAGAGCGGATTTGG370.15768165352652885No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA360.15341998721500105No Hit
AAGGAAGGGAAATCAACCGAGACCCCGTTAGTA360.15341998721500105No Hit
ATCCACACTACACACGTACTCTGCGTTGATACC340.14489665459194545No Hit
AACTGAAACATCTAAGTAGCTAAAGGAAGGGAA340.14489665459194545No Hit
TATCCACACTACACACGTACTCTGCGTTGATAC330.14063498828041765No Hit
ATCCATGGATCTCCGATCGGGAAACCGTATCCA330.14063498828041765No Hit
GGGGAGATACCGTCTGTGATCCATGGATCTCCG330.14063498828041765No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA330.14063498828041765No Hit
CCGTATCCAAGCTCCGTGGCTAGTCTGCGCTCT330.14063498828041765No Hit
GTGATCCATGGATCTCCGATCGGGAAACCGTAT320.13637332196888985No Hit
GAAACATCTAAGTAGCTAAAGGAAGGGAAATCA320.13637332196888985No Hit
CTGAAACATCTAAGTAGCTAAAGGAAGGGAAAT320.13637332196888985No Hit
ATGGATCTCCGATCGGGAAACCGTATCCAAGCT310.13211165565736202No Hit
CCATGGATCTCCGATCGGGAAACCGTATCCAAG310.13211165565736202No Hit
GTATCAACGCAGAGTGAATGGGGAATGCATTGG300.12784998934583422No Hit
CCCCTCCACACTACACACGTACTCTGCGTTGAT300.12784998934583422No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA290.12358832303430642No Hit
GAGAAGGAAGGACGCTTTCAGAGGCGAAAGGCC290.12358832303430642No Hit
ATTCCACACTACACACGTACTCTGCGTTGATAC280.11932665672277862No Hit
GGAAATCAACCGAGACCCCGTTAGTAGCGGCGA280.11932665672277862No Hit
GGGAAACCGTATCCAAGCTCCGTGGCTAGTCTG280.11932665672277862No Hit
GTCTGTGATCCATGGATCTCCGATCGGGAAACC280.11932665672277862No Hit
AATCCACACTACACACGTACTCTGCGTTGATAC270.1150649904112508No Hit
AATCAACCGAGACCCCGTTAGTAGCGGCGAGCG260.110803324099723No Hit
GATCCATGGATCTCCGATCGGGAAACCGTATCC260.110803324099723No Hit
CATTGGATGGATGCCCGGGCATTGAGAAGGAAG260.110803324099723No Hit
GTTCCACACTACACACGTACTCTGCGTTGATAC260.110803324099723No Hit
CCGATCGGGAAACCGTATCCAAGCTCCGTGGCT260.110803324099723No Hit
AACCGAGACCCCGTTAGTAGCGGCGAGCGAGAG250.10654165778819519No Hit
GGTCCACACTACACACGTACTCTGCGTTGATAC250.10654165778819519No Hit
CCACACTACACACGTACTCTGCGTTTATACCAC250.10654165778819519No Hit
GATCCACACTACACACGTACTCTGCGTTGATAC240.10227999147666737No Hit
CCGTTAGTAGCGGCGAGCGAGAGCGGATTTGGG240.10227999147666737No Hit
GCGCTCTTTGGACTTTGAAAACTTAGCGAACTG240.10227999147666737No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA240.10227999147666737No Hit
TACCGTCTGTGATCCATGGATCTCCGATCGGGA240.10227999147666737No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCAC356.0614857E-627.09421727
CTCTGCT257.540267E-427.03632520
TCTGCTT257.540267E-427.03632521
TGCTTTG257.540267E-427.03632523
CTGCTTT257.540267E-427.03632522
GCTTTGA257.540267E-427.03632524
TTTGATA257.540267E-427.03632526
GATACCA401.7369646E-523.65678426
CTTTGAT300.00220974722.48223125
CGTGTGT450.001072455217.9857861
TTGATAC2050.017.181727
ACTTTGA657.3076406E-414.526989
TCTTTGG657.3076406E-414.526982
GTTGATA2000.014.19407126
TGATACC802.1690819E-413.5471127
GGACTTT700.001280135413.489347
CTCTTTG700.001280135413.489341
TGGACTT700.001280135413.489346
TTTGGAC700.001280135413.489344
CTTTGGA700.001280135413.489343
Produced by FastQC (version 0.11.2)