FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate6_69.3520000005f5cd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate6_69.3520000005f5cd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72615
Sequences flagged as poor quality0
Sequence length33
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGC3050.4200234111409488No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCC2120.2919506988914136No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1530.21070026853955795No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCT1520.2093231426013909No Hit
GTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAG1120.1542381050747091No Hit
CCCCAGTTCAAGGTACGTACTCTGCGTTGATAC1090.15010672726020793No Hit
GCTAAATACGGGCGAGAGACCGATAGCGAACAA890.12256420849686704No Hit
GCGCTTAAGCGCGCGACCTATACCCGGCCGTCG840.11567857880603181No Hit
ACCTAGGGCGCGAGCCCGGGCGGAGCGGCCGTC840.11567857880603181No Hit
GGCTAAATACGGGCGAGAGACCGATAGCGAACA830.11430145286786476No Hit
GTTTGGGAATGCAGCCCCAATCGGGCGGTAAAT810.11154720099153068No Hit
GCCCCAATCGGGCGGTAAATTCCGTCCAAGGCT780.10741582317702954No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCCCA200.00856032826.9986235
GTATATG200.00856032826.9986231
AGAACCC200.00856032826.9986234
CACTCCA200.00856032826.9986235
GAGCCCG307.070336E-526.99862112
CGAGCCC257.7591836E-426.99862111
CGCACCC257.7591836E-426.9986214
GGAGCGG257.7591836E-426.99862122
GCGAGCC257.7591836E-426.99862110
CGCGAGC257.7591836E-426.9986219
AATCCAG356.4736523E-626.998626
CTATACC356.4736523E-626.998623
TATACCC356.4736523E-626.998624
GCACCCA356.4736523E-626.998625
CACCCAG701.0913936E-1125.0701476
CCTATAC401.8235065E-523.6237952
ACCCGGC352.0142147E-423.1416747
CCGGCCG352.0142147E-423.1416749
AGCCCGG300.002245027822.4988513
GAGCGGC300.002245027822.4988523
Produced by FastQC (version 0.11.2)