FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate6_66.3520000005f560.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate6_66.3520000005f560.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26825
Sequences flagged as poor quality0
Sequence length33
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCCAATGCACGTACTCTGCGTTGATACCA1480.5517241379310345No Hit
GTGAATGGGGTATCCAAAAAAAAAAAAAAAAAA1440.5368126747437092No Hit
GTGAATGGGTATCCAAAAAAAAAAAAAAAAAAA1120.4175209692451071No Hit
CCGGTATCCAATGCACGTACTCTGCGTTGATAC1050.39142590866728794No Hit
GTATCCAATGCACGTACTCTGCGTTGATACCAC1040.38769804287045667No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGC710.26467847157502333No Hit
GTGAATGGGTATCCAATGCACAAAAAAAAAAAA670.24976700838769805No Hit
TATCAACGCAGAGTGAATGGGTATCCAATGCAC580.21621621621621623No Hit
CTGGTATCCAATGCACGTACTCTGCGTTGATAC530.19757688723205966No Hit
GGGTATCCAATGCACGTACTCTGCGTTGATACC520.19384902143522834No Hit
GTGAATGGGTATCCAATGCACGTACTCAAAAAA520.19384902143522834No Hit
GTGAATGGGTATCCAATGAAAAAAAAAAAAAAA500.1863932898415657No Hit
CCAATGCACGTACTCAAAAAAAAAAAAAAAAAA480.17893755824790306No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA460.17148182665424044No Hit
CAGGTATCCAATGCACGTACTCTGCGTTGATAC450.16775396085740912No Hit
GTGAATGGGTATCCAATGCAAAAAAAAAAAAAA420.1565703634669152No Hit
GCGGTATCCAATGCACGTACTCTGCGTTGATAC420.1565703634669152No Hit
GTATCAACGCAGAGTGAATGGGTATCCAATGCA420.1565703634669152No Hit
CGGGTATCCAATGCACGTACTCTGCGTTGATAC400.14911463187325258No Hit
GTGGTATCCAATGCACGTACTCTGCGTTGATAC400.14911463187325258No Hit
CGGTATCCAATGCACGTACTCTGCGTTGATACC390.14538676607642123No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCT390.14538676607642123No Hit
ATGGTATCCAATGCACGTACTCTGCGTTGATAC370.13793103448275862No Hit
ACGGTATCCAATGCACGTACTCTGCGTTGATAC370.13793103448275862No Hit
GTGAATGGGTATCCAATGCACGTAAAAAAAAAA360.1342031686859273No Hit
ACGCAGAGTGAATGGGGTATCCAAAAAAAAAAA350.130475302889096No Hit
TATCAACGCAGAGTGAATGGGGTATCCAAAAAA350.130475302889096No Hit
TATCAACGCAGAGTGAATGGGGTATCCAATGCA330.12301957129543337No Hit
GTGAATGGGTATCCAATGCACGTACAAAAAAAA320.11929170549860205No Hit
TATCCAATGCACGTACTCTGCGTTGATACCACT300.11183597390493942No Hit
GGGGTATCCAATGCACGTACTCTGCGTTGATAC300.11183597390493942No Hit
GTGAATGGGGTATCCAATGCACGTACTCAAAAA290.10810810810810811No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCC270.10065237651444547No Hit
GTATCAACGCAGAGTGAATGGGGTATCCAAAAA270.10065237651444547No Hit
TCGGTATCCAATGCACGTACTCTGCGTTGATAC270.10065237651444547No Hit
GAGTGAATGGGGTATCCAAAAAAAAAAAAAAAA270.10065237651444547No Hit
TATCAACGCAGAGTGAATGGGTATCCAAAAAAA270.10065237651444547No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTGCT200.00846379927.020
CTGCTTT200.00846379927.022
CTTTGAT200.00846379927.025
GCTTTGA200.00846379927.024
CACGGTA200.00846379927.06
ACGGTAT559.104619E-619.6363647
CGGGTAT450.001072252918.0000027
TTGATAC1500.018.027
TCTGCGT5400.015.527
GTTGATA1454.5474735E-1114.89655226
CGTTGAT1551.3096724E-1013.93548425
TATCAAC700.001280935613.51
ATGGGTA1152.0477728E-612.9130435
GCGTTGA1705.675247E-1012.70588124
CTCTGCG6750.012.426
TGGGGTA1203.3007818E-612.3756
AATGGGT1255.2107316E-611.884
ACTCTGC7150.011.70629325
ATGGGGT1502.5249028E-711.75
GGGGTAT1401.4267789E-611.5714297
Produced by FastQC (version 0.11.2)