FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate6_61.3520000005f4b1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate6_61.3520000005f4b1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12661
Sequences flagged as poor quality0
Sequence length33
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACCATAGAGTCACGTACTCTGCGTTGATACC1301.0267751362451623No Hit
CCATAGAGTCAAAAAAAAAAAAAAAAAAAAAAA810.6397598925835242No Hit
ACCATAGAGTCACGTACTCTGCGTTGATACCAC780.6160650817470974No Hit
GCACCATAGAGTCACGTACTCTGCGTTGATACC660.5212858384013901No Hit
ACACCATAGAGTCACGTACTCTGCGTTGATACC600.47389621672853643No Hit
CCATAGAGAAAAAAAAAAAAAAAAAAAAAAAAA470.3712187031040202No Hit
GCACCATAGAGTCAAAAAAAAAAAAAAAAAAAA460.36332043282521126No Hit
CCATAGAGTCACGTACTCAAAAAAAAAAAAAAA440.3475238922675934No Hit
GCATTGGATGGATGCCCGGGCATTGAGAAGGAA440.3475238922675934No Hit
CATCTAAGTAGCTAAAGGAAGGGAAATCAACCG430.3396256219887845No Hit
CTCTTTGGACTTTGAAAACTTAGCGAACTGAAA430.3396256219887845No Hit
CGTTAGTAGCGGCGAGCGAGAGCGGATTTGGGA370.2922360003159308No Hit
GGGAGATACCGTCTGTGATCCATGGATCTCCGA350.2764394597583129No Hit
CACCATAGAGTCACGTACTCTGCGTTGATACCA340.26854118947950395No Hit
CCATAGAGTAAAAAAAAAAAAAAAAAAAAAAAA330.26064291920069504No Hit
TCCAAGCTCCGTGGCTAGTCTGCGCTCTTTGGA320.25274464892188614No Hit
TGGCTAGTCTGCGCTCTTTGGACTTTGAAAACT310.24484637864307718No Hit
TATCAACGCAGAGTGAATGGGCACCATAGAGTC280.22115156780665035No Hit
GCCATGGGGAGATACCGTCTGTGATCCATGGAT280.22115156780665035No Hit
GCACCATAGAGAAAAAAAAAAAAAAAAAAAAAA260.20535502724903246No Hit
GCTAAAGGAAGGGAAATCAACCGAGACCCCGTT240.1895584866914146No Hit
GAGTGAATGGGCACCATAGAGTCAAAAAAAAAA230.18166021641260563No Hit
ACTCTATGGTGCAAAAAAAAAAAAAAAAAAAAA230.18166021641260563No Hit
TCACCATAGAGTCACGTACTCTGCGTTGATACC230.18166021641260563No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA230.18166021641260563No Hit
CCATAGAAAAAAAAAAAAAAAAAAAAAAAAAAA230.18166021641260563No Hit
CCCCACCATAGAGTCACGTACTCTGCGTTGATA220.1737619461337967No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA220.1737619461337967No Hit
AGTCTGCGCTCTTTGGACTTTGAAAACTTAGCG220.1737619461337967No Hit
GCACCATAGAGTAAAAAAAAAAAAAAAAAAAAA220.1737619461337967No Hit
TATCCAAGCTCCGTGGCTAGTCTGCGCTCTTTG210.16586367585498776No Hit
CCCCGTTAGTAGCGGCGAGCGAGAGCGGATTTG210.16586367585498776No Hit
GAGTGAATGGGCACCATAGAGAAAAAAAAAAAA210.16586367585498776No Hit
CATCTAAGTAGCTAAAAAAAAAAAAAAAAAAAA200.15796540557617883No Hit
ACCGTATCCAAGCTCCGTGGCTAGTCTGCGCTC190.15006713529736987No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA180.14216886501856094No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA170.13427059473975198No Hit
GAGTGAATGGGCACCATAGAAAAAAAAAAAAAA170.13427059473975198No Hit
TAGTAGCGGCGAGCGAGAGCGGATTTGGGATTT170.13427059473975198No Hit
TATCAACGCAGAGTGAATGGGCACCATAGAAAA170.13427059473975198No Hit
GTGAATGGGCACCATAGAGAAAAAAAAAAAAAA170.13427059473975198No Hit
CTTTGGACTTTGAAAACTTAGCGAACTGAAACA170.13427059473975198No Hit
GAAATCAACCGAGACCCCGTTAGTAGCGGCGAG160.12637232446094307No Hit
GTCTGCGCTCTTTGGACTTTGAAAACTTAGCGA160.12637232446094307No Hit
TCTGTGATCCATGGATCTCCGATCGGGAAACCG160.12637232446094307No Hit
GTTTAAGCATATCAAAAAAAAAAAAAAAAAAAA160.12637232446094307No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA160.12637232446094307No Hit
GTGAATGGGCACCATAAAAAAAAAAAAAAAAAA150.11847405418213411No Hit
GTGAATGGGCACCATAGAGTCAAAAAAAAAAAA150.11847405418213411No Hit
AACGCAGAGTGAATGGGCACCATAGAGTCAAAA150.11847405418213411No Hit
GAGTGAATGGGCACCATAGAGTAAAAAAAAAAA150.11847405418213411No Hit
GTGAATGGGGAATGCATTGGATGGATGCCCGGG150.11847405418213411No Hit
GTATCAACGCAGAGTGAATGGGCACCATAGAGT140.11057578390332518No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA140.11057578390332518No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT140.11057578390332518No Hit
GAGATACCGTCTGTGATCCATGGATCTCCGATC140.11057578390332518No Hit
GAGAAGGAAGGACGCTTTCAGAGGCGAAAGGCC140.11057578390332518No Hit
AGCTAAAGGAAGGGAAATCAACCGAGACCCCGT140.11057578390332518No Hit
CCATAGAGTCACAAAAAAAAAAAAAAAAAAAAA140.11057578390332518No Hit
GTGAATGGGGCACCAAAAAAAAAAAAAAAAAAA140.11057578390332518No Hit
GGCCATGGGGAGATACCGTCTGTGATCCATGGA130.10267751362451623No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA130.10267751362451623No Hit
ACGCAGAGTGAATGGGCACCATAGAGTCAAAAA130.10267751362451623No Hit
CCGTATCCAAGCTCCGTGGCTAGTCTGCGCTCT130.10267751362451623No Hit
CCCCAGGTCAGGCGGGATTACCCGCTGAGTTTA130.10267751362451623No Hit
GATTTGGGATTTTAAAAAAAAAAAAAAAAAAAA130.10267751362451623No Hit
GATACCGTCTGTGATCCATGGATCTCCGATCGG130.10267751362451623No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCAC200.00829798927.027
GTATCAA500.00208685816.1999991
TGATACC603.7758984E-415.7527
TTGATAC603.7758984E-415.7526
ATCAACG603.7758984E-415.752
TATCAAC706.758451E-515.4285721
TCAACGC656.939372E-414.5384613
CAACGCA751.2178559E-414.4000014
AACGCAG905.7080213E-412.05
TCACCAT905.7080213E-412.08
TCTGCGT3500.011.57142827
ACGCAGA1204.137778E-511.256
CTCTGCG3800.010.65789526
ACTCTGC3950.010.25316425
CGCAGAG1205.350815E-410.1257
GTACTCT4300.09.41860523
CAGAGTG1150.00441648769.3913059
AGAGTGA1150.00441648769.39130510
GCAGAGT1150.00441648769.3913058
TACTCTG4350.09.31034624
Produced by FastQC (version 0.11.2)