FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate6_59.3520000005f48b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate6_59.3520000005f48b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46150
Sequences flagged as poor quality0
Sequence length33
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAATGGGACTGAGGCAAAAAAAAAAAAAAAA1610.3488624052004334No Hit
TATCAACGCAGAGTGAATGGGGCACTTACGTAC960.20801733477789816No Hit
GCACTTACGTACTCAAAAAAAAAAAAAAAAAAA940.20368364030335864No Hit
CTTACGTACTCAAAAAAAAAAAAAAAAAAAAAA860.18634886240520043No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA790.17118093174431204No Hit
GTATCAACGCAGAGTGAATGGGGCACTTACGTA790.17118093174431204No Hit
CTGAGGCACTTACGTACTCTGCGTTGATACCAC790.17118093174431204No Hit
TATCAACGCAGAGTGAATGGGACTGAGGCAAAA770.16684723726977246No Hit
GTGAATGGGACTGAGGCACAAAAAAAAAAAAAA740.16034669555796316No Hit
GTGAATGGGGCACTTACGTACTCAAAAAAAAAA740.16034669555796316No Hit
GAGTGAATGGGACTGAGGCAAAAAAAAAAAAAA720.1560130010834236No Hit
GAATGGGACTGAGGCAAAAAAAAAAAAAAAAAA710.15384615384615385No Hit
ACGCAGAGTGAATGGGACTGAGGCAAAAAAAAA600.13001083423618637No Hit
GACTGAGGCAAAAAAAAAAAAAAAAAAAAAAAA590.12784398699891658No Hit
GGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAA590.12784398699891658No Hit
GGACTGAGGCAAAAAAAAAAAAAAAAAAAAAAA540.1170097508125677No Hit
CACTGAGGCACTTACGTACTCTGCGTTGATACC490.10617551462621885No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCATC257.70262E-427.0065232
ATCATCG257.70262E-427.0065233
TCATCGC257.70262E-427.0065234
CAACTCA257.751796E-426.97721
TCAGAGT257.751796E-426.97725
CATCGCA257.751796E-426.97725
ACTCAGA351.9946924E-423.1484453
AACTCAG300.002228844422.5054342
CTCAGAG300.002228844422.5054344
GTATCAT300.002242997322.4809991
TCGCAGA300.002242997322.4809997
ATCGCAG300.002242997322.4809996
CCACACT608.74481E-720.2328995
CAGACTG450.001094572117.9847986
TCTGCGT13350.017.90319827
TATCAAC1600.017.7037871
CACACTG901.350636E-716.4860676
ACCACTG500.002216320516.186326
GCCACTG500.002216320516.186326
GCGTTGA1402.7284841E-1115.43229727
Produced by FastQC (version 0.11.2)