FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate6_57.3520000005f448.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate6_57.3520000005f448.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34148
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGTACCGTAACGTACTCTGCGTTGATACC3911.1450158135176292No Hit
CTACGTACCGTAACGTACTCTGCGTTGATACCA1880.5505446878294483No Hit
GCTACGTACCGTAACGTACTCTGCGTTGATACC1880.5505446878294483No Hit
ACTACGTACCGTAACGTACTCTGCGTTGATACC1580.4626918121119831No Hit
TCTACGTACCGTAACGTACTCTGCGTTGATACC1360.3982663699191754No Hit
CCCTACGTACCGTAACGTACTCTGCGTTGATAC760.22256061848424505No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCC760.22256061848424505No Hit
CCCCTACGTACCGTAACGTACTCTGCGTTGATA540.15813517629143728No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA510.14934988871969077No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGC510.14934988871969077No Hit
CTCTACGTACCGTAACGTACTCTGCGTTGATAC510.14934988871969077No Hit
GCTACGTACCGAAAAAAAAAAAAAAAAAAAAAA460.1347077427667799No Hit
GCTACGTACCGTAAAAAAAAAAAAAAAAAAAAA450.1317793135761977No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA440.12885088438561557No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT390.1142087384327047No Hit
GCTAAATACGGGCGAGAGACCGATAGCGAACAA380.11128030924212252No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA370.10835188005154037No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA360.10542345086095818No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA360.10542345086095818No Hit
CCTCTACGTACCGTAACGTACTCTGCGTTGATA350.10249502167037601No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATACC901.2732926E-1121.027
TTGATAC1251.4479156E-915.11999926
CCTCTAC550.004129785614.7272736
TATCAAC1007.1575014E-613.4999991
TCTGCGT6200.012.4112927
CAACGCA1153.0137086E-511.739134
CTCTACG1308.252908E-611.4230777
TCAACGC1204.655638E-511.253
ATCAACG1204.655638E-511.252
CACTACG850.0055489211.1176468
GTTGATA1853.4536788E-710.21621725
CGTTGAT1904.980975E-79.9473724
GTATCAA1258.559731E-49.7199991
AACGCAG1453.161425E-49.3103465
TCTACGT2656.2827894E-99.1698118
CTCTGCG8350.09.05389226
GCGTTGA2203.6660822E-68.59090923
ACTCTGC9000.08.2525
ACTACGT2655.910715E-78.1509449
GTAACGT9950.08.00502518
Produced by FastQC (version 0.11.2)