FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate6_56.3520000005f42e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate6_56.3520000005f42e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13263
Sequences flagged as poor quality0
Sequence length33
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGAGCACTGTACGTACTCTGCGTTGATACCA4563.4381361682877176No Hit
GCACTGTACGTACAAAAAAAAAAAAAAAAAAAA1751.3194601523034004No Hit
TATCAACGCAGAGTGAATGGGCACTGTACGTAC1491.1234260725326095No Hit
GTGAATGGGCACTGTACGTACAAAAAAAAAAAA1210.9123124481640654No Hit
GTATCAACGCAGAGTGAATGGGCACTGTACGTA980.7388976852899043No Hit
GTACGTACTCAAAAAAAAAAAAAAAAAAAAAAA850.6408806454045087No Hit
CTGTACGTACAAAAAAAAAAAAAAAAAAAAAAA850.6408806454045087No Hit
CCCATGAGCACTGTACGTACTCTGCGTTGATAC830.6258011008067557No Hit
GCACTGTACGTACTCAAAAAAAAAAAAAAAAAA830.6258011008067557No Hit
ACGCAGAGTGAATGGGCACTGTACGTACAAAAA770.5805624670134962No Hit
GAGTGAATGGGCACTGTACGTACAAAAAAAAAA680.512704516323607No Hit
CCATGAGCACTGTACGTACTCTGCGTTGATACC640.4825454271281007No Hit
GTACGTACAAAAAAAAAAAAAAAAAAAAAAAAA480.36190907034607556No Hit
GTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA460.34682952574832243No Hit
CTGTACGTACTCAAAAAAAAAAAAAAAAAAAAA460.34682952574832243No Hit
GCCATGAGCACTGTACGTACTCTGCGTTGATAC430.32421020885169266No Hit
GTGAATGGGCACTGTACGTACTCAAAAAAAAAA420.31667043655281607No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA390.29405111965618635No Hit
GGTATCAACGCAGAGTGAATGGGCACTGTACGT340.2563522581618035No Hit
ACCATGAGCACTGTACGTACTCTGCGTTGATAC330.24881248586292692No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA320.24127271356405036No Hit
ACGCAGAGTGAATGGGCACTGTACGTACTCAAA290.21865339666742065No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT290.21865339666742065No Hit
ACGCAGAGTGAATGGGCACTGTACGTAAAAAAA280.21111362436854408No Hit
AACGCAGAGTGAATGGGCACTGTACGTACAAAA280.21111362436854408No Hit
GAATGGGCACTGTACGTACAAAAAAAAAAAAAA280.21111362436854408No Hit
TATCAACGCAGAGTGAATGGGCACTGTACGTAA280.21111362436854408No Hit
GCATGAGCACTGTACGTACTCTGCGTTGATACC270.2035738520696675No Hit
GTGAATGGGCACTGTACGTAAAAAAAAAAAAAA260.19603407977079093No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA260.19603407977079093No Hit
GTACGTACCTAAAAAAAAAAAAAAAAAAAAAAA250.18849430747191434No Hit
GTACTACTCAAAAAAAAAAAAAAAAAAAAAAAA240.18095453517303778No Hit
GCACTGTACGTACTAAAAAAAAAAAAAAAAAAA240.18095453517303778No Hit
GTCATGAGCACTGTACGTACTCTGCGTTGATAC240.18095453517303778No Hit
GCACTGTACGTAAAAAAAAAAAAAAAAAAAAAA240.18095453517303778No Hit
GAGCACTGTACGTACTCTGCGTTGATACCACTG220.16587499057528463No Hit
GTATCATCGCAGAGTGAATGGGCACTGTACGTA220.16587499057528463No Hit
CTCATGAGCACTGTACGTACTCTGCGTTGATAC220.16587499057528463No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA210.15833521827640804No Hit
GTACGTACCTGCAAAAAAAAAAAAAAAAAAAAA200.15079544597753147No Hit
GCACTGTACGTACCTAAAAAAAAAAAAAAAAAA190.1432556736786549No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA190.1432556736786549No Hit
GTACGTACCTGAAAAAAAAAAAAAAAAAAAAAA190.1432556736786549No Hit
GAGTGAATGGGCACTGTACGTAAAAAAAAAAAA180.13571590137977835No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA180.13571590137977835No Hit
GCACTGTACGTACCAAAAAAAAAAAAAAAAAAA180.13571590137977835No Hit
ATCAACGCAGAGTGAATGGGCACTGTACGTACA180.13571590137977835No Hit
GAGTGAATGGGCACTGTACGTACTCAAAAAAAA180.13571590137977835No Hit
GTACAGTGCTCAAAAAAAAAAAAAAAAAAAAAA180.13571590137977835No Hit
AATGGGCACTGTACGTACAAAAAAAAAAAAAAA170.12817612908090176No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA170.12817612908090176No Hit
GAATGGGCACTGTACGTACTCAAAAAAAAAAAA160.12063635678202518No Hit
GTACTACAAAAAAAAAAAAAAAAAAAAAAAAAA150.1130965844831486No Hit
CACTGTACGTACTCAAAAAAAAAAAAAAAAAAA150.1130965844831486No Hit
AACGCAGAGTGAATGGGCACTGTACGTACTCAA150.1130965844831486No Hit
TATCAACGCAGAGTGAATGGGCACTTTACGTAC150.1130965844831486No Hit
GTACGTACCAAAAAAAAAAAAAAAAAAAAAAAA140.10555681218427204No Hit
GTACGTAAAAAAAAAAAAAAAAAAAAAAAAAAA140.10555681218427204No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA140.10555681218427204No Hit
CGCATGAGCACTGTACGTACTCTGCGTTGATAC140.10555681218427204No Hit
TCCATGAGCACTGTACGTACTCTGCGTTGATAC140.10555681218427204No Hit
CAACGCAGAGTGAATGGGCACTGTACGTACAAA140.10555681218427204No Hit
CACATGAGCACTGTACGTACTCTGCGTTGATAC140.10555681218427204No Hit
CATGAGCACAAAAAAAAAAAAAAAAAAAAAAAA140.10555681218427204No Hit
TATCTACGCAGAGTGAATGGGCACTGTACGTAC140.10555681218427204No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACCA955.2023097E-1018.47368427
GTATCAA806.25605E-716.8751
TGATACC1051.9754225E-916.71428526
GTTGATA1103.6670826E-915.95454624
CGTTGAT1103.6670826E-915.95454623
TTGATAC1201.1612428E-814.62525
TATCAAC1051.0589427E-512.8571422
GCGTTGA1408.817915E-812.53571422
ATCAACG1101.7100581E-512.2727273
TCTGCGT3250.011.215384527
TGCGTTG1605.0167E-710.9687521
TCAACGC1309.4338844E-510.3846154
CAACGCA1401.9977624E-49.6428585
CTGCGTT1904.5714114E-69.23684220
AACGCAG1651.0067289E-49.06
CTCTGCG4200.08.67857226
ACTCTGC4550.08.01098925
GTACTCT4750.07.95789523
CGCAGAG2253.878253E-57.88
TACTCTG4700.07.755319624
Produced by FastQC (version 0.11.2)