FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate6_55.3520000005f404.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate6_55.3520000005f404.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46808
Sequences flagged as poor quality0
Sequence length33
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTGCTGAAGACGTACTCTGCGTTGATACCA3400.7263715604170228No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCC1450.30977610664843613No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1390.29695778499401815No Hit
TATCAACGCAGAGTGAATGGGCATTGCTGAAGA1320.28200307639719707No Hit
GCTGAAGACGTACTCAAAAAAAAAAAAAAAAAA1310.27986668945479404No Hit
GTATCAACGCAGAGTGAATGGGCATTGCTGAAG1050.22432062895231583No Hit
GTGAATGGGCATTGCTGAAGAAAAAAAAAAAAA960.20509314647068877No Hit
GTGAATGGGCATTGCTGAAAAAAAAAAAAAAAA860.1837292770466587No Hit
GCTGAAGACGTACAAAAAAAAAAAAAAAAAAAA790.16877456844983765No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGC710.15168347291061357No Hit
ACGCAGAGTGAATGGGCATTGCTGAAGAAAAAA620.13245599042898648No Hit
GGCTAAATACGGGCGAGAGACCGATAGCGAACA610.1303196034865835No Hit
ACGCAGAGTGAATGGGCATTGCTGAAAAAAAAA580.12391044265937447No Hit
GCTAAATACGGGCGAGAGACCGATAGCGAACAA540.11536489488976243No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT540.11536489488976243No Hit
GTTTGGGAATGCAGCCCCAATCGGGCGGTAAAT530.11322850794735942No Hit
TATCAACGCAGAGTGAATGGGCATTGCTGAAAA520.11109212100495641No Hit
GAGTGAATGGGCATTGCTGAAGAAAAAAAAAAA510.10895573406255342No Hit
ACGCAGAGTGAATGGGCATTGCTGAAGACGTAC500.10681934712015041No Hit
CCGCATTGCTGAAGACGTACTCTGCGTTGATAC500.10681934712015041No Hit
AACAAGTACCGCGAGGTAAAAAAAAAAAAAAAA500.10681934712015041No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA500.10681934712015041No Hit
GCTGAAGACAAAAAAAAAAAAAAAAAAAAAAAA480.10254657323534438No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTGTT200.0085276327.013
GGGTTGT200.0085276327.012
AGGGCAT200.0085276327.06
CGGGTTG200.0085276327.011
TCGGGTT200.0085276327.010
GATACCA454.4825752E-521.027
TGATACC454.4825752E-521.026
AGGCATT454.4825752E-521.07
CGCGCAT404.9290154E-420.256
CTACGAG350.00545548919.2857133
GTCGGGG350.00545548919.28571315
GCGCATT952.4770998E-715.6315797
TCTGCGT8850.015.4067827
GTATCAA1501.3315002E-913.5000011
AAGCATT904.1016843E-513.57
CTCTGCG10350.013.17391326
CTGCATT1051.2069802E-512.8571427
TATCAAC1603.4906407E-912.656251
ACTCTGC11500.011.85652225
ATCAACG1604.8046786E-811.81252