Basic Statistics
Measure | Value |
---|---|
Filename | HTC23BCXX_l02n02_kb_plate6_55.3520000005f404.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 46808 |
Sequences flagged as poor quality | 0 |
Sequence length | 33 |
%GC | 38 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GCATTGCTGAAGACGTACTCTGCGTTGATACCA | 340 | 0.7263715604170228 | No Hit |
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCC | 145 | 0.30977610664843613 | No Hit |
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 139 | 0.29695778499401815 | No Hit |
TATCAACGCAGAGTGAATGGGCATTGCTGAAGA | 132 | 0.28200307639719707 | No Hit |
GCTGAAGACGTACTCAAAAAAAAAAAAAAAAAA | 131 | 0.27986668945479404 | No Hit |
GTATCAACGCAGAGTGAATGGGCATTGCTGAAG | 105 | 0.22432062895231583 | No Hit |
GTGAATGGGCATTGCTGAAGAAAAAAAAAAAAA | 96 | 0.20509314647068877 | No Hit |
GTGAATGGGCATTGCTGAAAAAAAAAAAAAAAA | 86 | 0.1837292770466587 | No Hit |
GCTGAAGACGTACAAAAAAAAAAAAAAAAAAAA | 79 | 0.16877456844983765 | No Hit |
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGC | 71 | 0.15168347291061357 | No Hit |
ACGCAGAGTGAATGGGCATTGCTGAAGAAAAAA | 62 | 0.13245599042898648 | No Hit |
GGCTAAATACGGGCGAGAGACCGATAGCGAACA | 61 | 0.1303196034865835 | No Hit |
ACGCAGAGTGAATGGGCATTGCTGAAAAAAAAA | 58 | 0.12391044265937447 | No Hit |
GCTAAATACGGGCGAGAGACCGATAGCGAACAA | 54 | 0.11536489488976243 | No Hit |
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT | 54 | 0.11536489488976243 | No Hit |
GTTTGGGAATGCAGCCCCAATCGGGCGGTAAAT | 53 | 0.11322850794735942 | No Hit |
TATCAACGCAGAGTGAATGGGCATTGCTGAAAA | 52 | 0.11109212100495641 | No Hit |
GAGTGAATGGGCATTGCTGAAGAAAAAAAAAAA | 51 | 0.10895573406255342 | No Hit |
ACGCAGAGTGAATGGGCATTGCTGAAGACGTAC | 50 | 0.10681934712015041 | No Hit |
CCGCATTGCTGAAGACGTACTCTGCGTTGATAC | 50 | 0.10681934712015041 | No Hit |
AACAAGTACCGCGAGGTAAAAAAAAAAAAAAAA | 50 | 0.10681934712015041 | No Hit |
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA | 50 | 0.10681934712015041 | No Hit |
GCTGAAGACAAAAAAAAAAAAAAAAAAAAAAAA | 48 | 0.10254657323534438 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GGTTGTT | 20 | 0.00852763 | 27.0 | 13 |
GGGTTGT | 20 | 0.00852763 | 27.0 | 12 |
AGGGCAT | 20 | 0.00852763 | 27.0 | 6 |
CGGGTTG | 20 | 0.00852763 | 27.0 | 11 |
TCGGGTT | 20 | 0.00852763 | 27.0 | 10 |
GATACCA | 45 | 4.4825752E-5 | 21.0 | 27 |
TGATACC | 45 | 4.4825752E-5 | 21.0 | 26 |
AGGCATT | 45 | 4.4825752E-5 | 21.0 | 7 |
CGCGCAT | 40 | 4.9290154E-4 | 20.25 | 6 |
CTACGAG | 35 | 0.005455489 | 19.285713 | 3 |
GTCGGGG | 35 | 0.005455489 | 19.285713 | 15 |
GCGCATT | 95 | 2.4770998E-7 | 15.631579 | 7 |
TCTGCGT | 885 | 0.0 | 15.40678 | 27 |
GTATCAA | 150 | 1.3315002E-9 | 13.500001 | 1 |
AAGCATT | 90 | 4.1016843E-5 | 13.5 | 7 |
CTCTGCG | 1035 | 0.0 | 13.173913 | 26 |
CTGCATT | 105 | 1.2069802E-5 | 12.857142 | 7 |
TATCAAC | 160 | 3.4906407E-9 | 12.65625 | 1 |
ACTCTGC | 1150 | 0.0 | 11.856522 | 25 |
ATCAACG | 160 | 4.8046786E-8 | 11.8125 | 2 |