FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate6_54.3520000005f3e9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate6_54.3520000005f3e9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14883
Sequences flagged as poor quality0
Sequence length33
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTACGATCAGACGTACTCTGCGTTGATACCAC1961.316938789222603No Hit
GCATTGGATGGATGCCCGGGCATTGAGAAGGAA780.5240878855069542No Hit
CTCTTTGGACTTTGAAAACTTAGCGAACTGAAA640.43002082913391115No Hit
GATCAGACGTACTCAAAAAAAAAAAAAAAAAAA620.4165826782234765No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT620.4165826782234765No Hit
GGGAGATACCGTCTGTGATCCATGGATCTCCGA620.4165826782234765No Hit
CATCTAAGTAGCTAAAGGAAGGGAAATCAACCG610.40986360276825906No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA610.40986360276825906No Hit
CGTTAGTAGCGGCGAGCGAGAGCGGATTTGGGA600.40314452731304173No Hit
TGGCTAGTCTGCGCTCTTTGGACTTTGAAAACT590.3964254518578243No Hit
GCTAAAGGAAGGGAAATCAACCGAGACCCCGTT570.38298730094738964No Hit
GTGCTACGATCAGACGTACTCTGCGTTGATACC560.3762682254921723No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA520.34939192367130284No Hit
CTGCTACGATCAGACGTACTCTGCGTTGATACC500.3359537727608681No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA480.32251562185043336No Hit
GCCATGGGGAGATACCGTCTGTGATCCATGGAT450.30235839548478133No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA440.29563932002956395No Hit
AGCTAAAGGAAGGGAAATCAACCGAGACCCCGT420.28220116911912924No Hit
CGTCTGATCGTAGCAAAAAAAAAAAAAAAAAAA410.27548209366391185No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA360.24188671638782502No Hit
TATCCAAGCTCCGTGGCTAGTCTGCGCTCTTTG310.20829133911173825No Hit
ATGCTACGATCAGACGTACTCTGCGTTGATACC310.20829133911173825No Hit
GATTTGGGATTTTAAAAAAAAAAAAAAAAAAAA310.20829133911173825No Hit
CTTTGGACTTTGAAAACTTAGCGAACTGAAACA310.20829133911173825No Hit
GACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA300.20157226365652087No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA300.20157226365652087No Hit
TATCAACGCAGAGTGAATGGGATCAGACGTACT300.20157226365652087No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA290.19485318820130348No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA290.19485318820130348No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA290.19485318820130348No Hit
CATCTAAGTAGCTAAAAAAAAAAAAAAAAAAAA270.18141503729086877No Hit
TGCTACGATCAGACGTACTCTGCGTTGATACCA260.17469596183565142No Hit
TCTGTGATCCATGGATCTCCGATCGGGAAACCG260.17469596183565142No Hit
TCCAAGCTCCGTGGCTAGTCTGCGCTCTTTGGA260.17469596183565142No Hit
ACCGAGACCCCGTTAGTAGCGGCGAGCGAGAGC260.17469596183565142No Hit
GAAATCAACCGAGACCCCGTTAGTAGCGGCGAG250.16797688638043404No Hit
GATCGTAGCAAAAAAAAAAAAAAAAAAAAAAAA250.16797688638043404No Hit
GAGTACGTCTGATCGTAGCAAAAAAAAAAAAAA250.16797688638043404No Hit
GCTACGATCAGACGTACTCTGCGTTTATACCAC230.15453873546999933No Hit
ACCGTATCCAAGCTCCGTGGCTAGTCTGCGCTC230.15453873546999933No Hit
GAGTGAATGGGATCAGACGTACTCAAAAAAAAA220.14781966001478197No Hit
GTCTGCGCTCTTTGGACTTTGAAAACTTAGCGA210.14110058455956462No Hit
GAGATACCGTCTGTGATCCATGGATCTCCGATC210.14110058455956462No Hit
GTGCTACAAAAAAAAAAAAAAAAAAAAAAAAAA210.14110058455956462No Hit
GTGAATGGGGAATGCATTGGATGGATGCCCGGG210.14110058455956462No Hit
GGCGAAAGGCCATGGGGAGATACCGTCTGTGAT200.13438150910434724No Hit
GAGAAGGAAGGACGCTTTCAGAGGCGAAAGGCC200.13438150910434724No Hit
CTTTGAAAACTTAGCGAACTGAAACATCTAAGT200.13438150910434724No Hit
CCCGTTAGTAGCGGCGAGCGAGAGCGGATTTGG190.12766243364912988No Hit
GACGTACTCTGCGAAAAAAAAAAAAAAAAAAAA180.12094335819391251No Hit
AGTCTGCGCTCTTTGGACTTTGAAAACTTAGCG180.12094335819391251No Hit
AGCGAGAGCGGATTTGGGATTTTAAAAAAAAAA180.12094335819391251No Hit
GATCCATGGATCTCCGATCGGGAAACCGTATCC180.12094335819391251No Hit
GACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA180.12094335819391251No Hit
CCATGGATCTCCGATCGGGAAACCGTATCCAAG180.12094335819391251No Hit
GTATCAACGCAGAGTGAATGGGATCAGACGTAC170.11422428273869516No Hit
GGCATTGAGAAGGAAGGACGCTTTCAGAGGCGA170.11422428273869516No Hit
AAGGAAGGGAAATCAACCGAGACCCCGTTAGTA170.11422428273869516No Hit
GGATGGATGCCCGGGCATTGAGAAGGAAGGACG170.11422428273869516No Hit
CTCCGTGGCTAGTCTGCGCTCTTTGGACTTTGA170.11422428273869516No Hit
GGTCTGATCGTAGCAAAAAAAAAAAAAAAAAAA160.10750520728347779No Hit
GTTTAAGCATATCAAAAAAAAAAAAAAAAAAAA160.10750520728347779No Hit
CCCCAGGTCAGGCGGGATTACCCGCTGAGTTTA160.10750520728347779No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA150.10078613182826043No Hit
CCCCGTTAGTAGCGGCGAGCGAGAGCGGATTTG150.10078613182826043No Hit
CCGTATCCAAGCTCCGTGGCTAGTCTGCGCTCT150.10078613182826043No Hit
AACTGAAACATCTAAGTAGCTAAAGGAAGGGAA150.10078613182826043No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCAC356.026441E-627.027
GATACCA401.6986973E-523.62526
GTTGATA657.040531E-414.53846223
TGATACC657.040531E-414.53846225
TTGATAC700.001233754113.524
TATCAAC1050.002113288710.2857142
TCTGCGT2952.7284841E-119.61016927
GTATCAA1200.0063826119.01
CAGAGTG1607.7600253E-48.43754
AGAGTGA1607.7600253E-48.43755
GCAGAGT1607.7600253E-48.43753
ACGCAGA1956.666684E-58.3076931
CGCAGAG1853.5629855E-48.0270272
CTCTGCG3804.1636667E-97.460526526
CTGCTAC1650.0098740197.36363658
GTGAATG2757.4307827E-67.3636361
TGAATGG2809.592388E-67.2321432
AGTGAAT2000.0069638466.757
ACTCTGC4051.1286647E-76.66666725
GAGTGAA2050.0088449256.5853666
Produced by FastQC (version 0.11.2)