FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate6_48.3520000005f312.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate6_48.3520000005f312.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24064
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCCACGCTTAACGTACTCTGCGTTGATACCA2761.146941489361702No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA390.16206781914893617No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT380.15791223404255317No Hit
CGCTTAACGTACTCAAAAAAAAAAAAAAAAAAA340.1412898936170213No Hit
GTGAATGGGGTTCCACAAAAAAAAAAAAAAAAA330.1371343085106383No Hit
GTGAATGGGTTCCACAAAAAAAAAAAAAAAAAA320.13297872340425532No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCC250.10388962765957446No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATACC601.1859811E-924.7526
GATACCA551.3038516E-824.54545427
GTTGATA603.284913E-822.524
TTGATAC603.284913E-822.525
CGTTGAT701.6756348E-719.28571523
GCGTTGA1507.4578566E-1114.40000127
TATCAAC1255.14758E-611.8799991
AACGCAG1204.5236557E-511.255
TCAACGC1204.5236557E-511.253
ATCAACG1204.5236557E-511.252
CAACGCA1204.5236557E-511.254
GTATCAA1000.001464254910.7999991
TGCGTTG2358.174538E-99.76595727
ACGCAGA1555.9949124E-48.7096776
CTGCGTT2655.804759E-88.66037725
TCTGCGT3901.8189894E-128.65384627
TGTTCCA1250.0092731518.6399995
CAGAGTG1450.0032371878.379319
AGAGTGA1450.0032371878.3793110
CGCAGAG1650.00111637038.1818187
Produced by FastQC (version 0.11.2)