FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate6_47.3520000005f2f7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate6_47.3520000005f2f7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26825
Sequences flagged as poor quality0
Sequence length33
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTACGTTAGCAAAAAAAAAAAAAAAAAAAAA750.27958993476234856No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA640.2385834109972041No Hit
CCTCCAGTCTGCTAACGTACTCTGCGTTGATAC630.23485554520037277No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT590.21994408201304752No Hit
GTCCAGTCTGCTAACGTACTCTGCGTTGATACC570.2124883504193849No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA550.20503261882572227No Hit
CTCCAGTCTGCTAACGTACTCTGCGTTGATACC530.19757688723205966No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCC450.16775396085740912No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA440.16402609506057783No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA400.14911463187325258No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA380.14165890027958994No Hit
GTACGTTAGCAAAAAAAAAAAAAAAAAAAAAAA340.1267474370922647No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA340.1267474370922647No Hit
GTGAATGGGGAGTACGTTAGCAAAAAAAAAAAA340.1267474370922647No Hit
GTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA290.10810810810810811No Hit
CCCCAGGTCAGGCGGGATTACCCGCTGAGTTTA280.10438024231127678No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA270.10065237651444547No Hit
GTCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA270.10065237651444547No Hit
CTTCCAGTCTGCTAACGTACTCTGCGTTGATAC270.10065237651444547No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTTAC200.00846379927.023
TAACTTA200.00846379927.022
CTAACTT200.00846379927.021
GCTAACT200.00846379927.020
GGCTCCA200.00846379927.06
GACCTCC200.00846379927.05
TTGATAC450.001072252918.00000227
GTTGATA500.002171550416.226
TGCGTTG2200.015.95454627
TATCAAC1251.3951649E-915.121
TCTGCGT8050.014.75776427
CTTCCAG1107.5855496E-814.7272737
AACGCAG1457.348717E-1013.9655185
TCAACGC1406.901246E-913.53
ATTCCAG802.2537695E-413.57
CAACGCA1406.901246E-913.54
TTCCAGT2650.013.2452838
ATCAACG1451.1295924E-813.0344832
ACCTCCA750.002153883512.5999996
CTCTGCG9550.012.4397926
Produced by FastQC (version 0.11.2)