FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate6_44.3520000005f29a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate6_44.3520000005f29a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27486
Sequences flagged as poor quality0
Sequence length33
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAATGGGCAGAGTGGCAAAAAAAAAAAAAAA620.22556938077566763No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCC540.19646365422396855No Hit
GGCTAAACGTACTCAAAAAAAAAAAAAAAAAAA530.19282543840500618No Hit
TATCAACGCAGAGTGAATGGGCAGAGTGGCAAA520.18918722258604379No Hit
ACGCAGAGTGAATGGGCAGAGTGGCAAAAAAAA430.15644328021538237No Hit
GAGTGGAAAAAAAAAAAAAAAAAAAAAAAAAAA350.12733755366368332No Hit
GAGTGGCAAAAAAAAAAAAAAAAAAAAAAAAAA320.11642290620679618No Hit
GTGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA320.11642290620679618No Hit
GTGAATGGGCAGAGTGGAAAAAAAAAAAAAAAA300.10914647456887142No Hit
TATCAACGCAGAGTGAATGGGCTAAACGTACTC290.10550825874990906No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCAGA351.9694348E-423.1428597
GTATCAA559.1179645E-619.6363641
TCTGCGT6150.019.53658527
GACCAGA350.005399259719.2857156
CGTTGAT856.66696E-817.47058927
TATCAAC1204.1836756E-1116.8751
GCGTTGA901.2803503E-716.50000226
ACCAGAG1600.015.18757
CTCTGCG8800.013.65340926
ATCAACG1501.2296368E-913.5000012
CACCAGA802.2569341E-413.56
TCAACGC1552.0081643E-913.0645173
ACTCTGC9500.012.50526225
GTACTCT10000.012.15000123
TACTCTG10050.011.95522324
CAACGCA1707.954441E-911.9117654
CGTACTC10400.011.68269222
CCCAGAG1852.1718733E-911.6756758
CCAGAGT5000.011.6100018
ACGTACT10550.011.51658821
Produced by FastQC (version 0.11.2)