FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate6_43.3520000005f270.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate6_43.3520000005f270.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25085
Sequences flagged as poor quality0
Sequence length33
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCCAGCTAGACGTACTCTGCGTTGATACCAC2090.8331672314131952No Hit
GTGAATGGGACATCCAGCAAAAAAAAAAAAAAA830.33087502491528803No Hit
ACATCCAGCTAGACGTACTCTGCGTTGATACCA610.24317321108232012No Hit
ATCCAGCTAGACGTACTCTGCGTTGATACCACT550.21925453458241975No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA450.17939007374925253No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA440.1754036276659358No Hit
GTGAATGGGACATCCAGCTAGAAAAAAAAAAAA420.16743073549930237No Hit
ACTATATTGTGTGTTGTTTGTATCCGCTCGCTA410.16344428941598566No Hit
GTGAATGGGACATCCAGAAAAAAAAAAAAAAAA380.1514849511660355No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA370.14749850508271875No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT370.14749850508271875No Hit
GTGAATGGGACATCCAAAAAAAAAAAAAAAAAA360.14351205899940203No Hit
TATCAACGCAGAGTGAATGGGACATCCAGCTAG360.14351205899940203No Hit
ACGCAGAGTGAATGGGACATCCAGCAAAAAAAA310.12357982858281842No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA310.12357982858281842No Hit
CCCCCACATCCAGCTAGACGTACTCTGCGTTGA300.11959338249950169No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA280.11162049033286825No Hit
GTGAATGGGGACATCCAAAAAAAAAAAAAAAAA270.10763404424955153No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA270.10763404424955153No Hit
CCCCACATCCAGCTAGACGTACTCTGCGTTGAT270.10763404424955153No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA270.10763404424955153No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCAC200.00845338327.027
TGCACAT300.002203819322.56
GCACATC901.2670534E-716.5000027
GTTGATA801.3003555E-515.187527
ATCAACG1352.4738256E-1015.02
TATCAAC1454.3655746E-1114.8965521
TGGGACA600.00728365313.56
TCTGCGT6500.012.6692327
TCAACGC1603.1668606E-912.656253
CAACGCA1603.1668606E-912.656254
AACGCAG1852.1263986E-911.6756755
GTATCAA1051.673759E-411.5714281
TGCGTTG3000.011.25000127
AATGGGA1452.1585747E-611.1724144
GGGACAT850.005479240311.1176477
CTCTGCG8100.010.526
GCAGAGT1955.9284503E-810.3846168
CAGAGTG1853.2962998E-710.2162169
CGTTGAT1351.5024605E-410.027
ACTCTGC8550.09.94736925
Produced by FastQC (version 0.11.2)