FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate6_40.3520000005f213.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate6_40.3520000005f213.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37748
Sequences flagged as poor quality0
Sequence length33
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTGGATGGATGCCCGGGCATTGAGAAGGAA3931.041114761046943No Hit
GCTCAACACGTACTCAAAAAAAAAAAAAAAAAA3470.9192540002119318No Hit
GGGAGATACCGTCTGTGATCCATGGATCTCCGA2950.7814983575288756No Hit
CATCTAAGTAGCTAAAGGAAGGGAAATCAACCG2700.7152696831620218No Hit
GTCAAGCTCAACACGTACTCTGCGTTGATACCA2300.6093038041750556No Hit
GCCATGGGGAGATACCGTCTGTGATCCATGGAT2240.5934089223270107No Hit
TGGCTAGTCTGCGCTCTTTGGACTTTGAAAACT1690.4477058387199322No Hit
GCTAAAGGAAGGGAAATCAACCGAGACCCCGTT1670.4424075447705839No Hit
CTCTTTGGACTTTGAAAACTTAGCGAACTGAAA1640.4344601038465614No Hit
GTTTAAGCATATCAAAAAAAAAAAAAAAAAAAA1600.42386351594786476No Hit
GTGAATGGGTCAAGCTCAAAAAAAAAAAAAAAA1590.4212143689731906No Hit
CGTTAGTAGCGGCGAGCGAGAGCGGATTTGGGA1440.3814771643530783No Hit
CCCCAGGTCAGGCGGGATTACCCGCTGAGTTTA1370.3629331355303592No Hit
CTTTGGACTTTGAAAACTTAGCGAACTGAAACA1320.34968740065698845No Hit
GTGAATGGGGAATGCATTGGATGGATGCCCGGG1210.32054678393557273No Hit
TATCCAAGCTCCGTGGCTAGTCTGCGCTCTTTG1140.3020027551128537No Hit
AGCTAAAGGAAGGGAAATCAACCGAGACCCCGT1140.3020027551128537No Hit
TCCAAGCTCCGTGGCTAGTCTGCGCTCTTTGGA1120.29670446116350535No Hit
TATCAACGCAGAGTGAATGGGTCAAGCTCAACA1110.29405531418883124No Hit
CATCTAAGTAGCTAAAAAAAAAAAAAAAAAAAA1110.29405531418883124No Hit
ACCGTATCCAAGCTCCGTGGCTAGTCTGCGCTC1030.27286213839143797No Hit
TCTGTGATCCATGGATCTCCGATCGGGAAACCG950.25166896259404475No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT930.2463706686446964No Hit
TATCAACGCAGAGTGAATGGGTCAAGCTCAAAA920.24372152167002223No Hit
GGCGAAAGGCCATGGGGAGATACCGTCTGTGAT880.23312493377132562No Hit
GGATGGATGCCCGGGCATTGAGAAGGAAGGACG850.22517749284730318No Hit
GTCAAGCTCAAAAAAAAAAAAAAAAAAAAAAAA840.222528345872629No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA830.21987919889795488No Hit
GAGATACCGTCTGTGATCCATGGATCTCCGATC830.21987919889795488No Hit
GATTTGGGATTTTAAAAAAAAAAAAAAAAAAAA820.2172300519232807No Hit
GAGTTTAAGCATATCAAAAAAAAAAAAAAAAAA810.21458090494860657No Hit
GGCATTGAGAAGGAAGGACGCTTTCAGAGGCGA800.21193175797393238No Hit
AGTCTGCGCTCTTTGGACTTTGAAAACTTAGCG790.2092826109992582No Hit
GATACCGTCTGTGATCCATGGATCTCCGATCGG770.2039843170499099No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCC720.19073858217653916No Hit
GGCCATGGGGAGATACCGTCTGTGATCCATGGA710.188089435201865No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA710.188089435201865No Hit
ACGCAGAGTGAATGGGTCAAGCTCAAAAAAAAA700.18544028822719086No Hit
CCCCGTTAGTAGCGGCGAGCGAGAGCGGATTTG670.1774928473031684No Hit
GAGAAGGAAGGACGCTTTCAGAGGCGAAAGGCC660.17484370032849422No Hit
GTCTGCGCTCTTTGGACTTTGAAAACTTAGCGA650.17219455335382008No Hit
AACGCAGAGTGAATGGGTCAAGCTCAAAAAAAA640.16954540637914592No Hit
ACCGAGACCCCGTTAGTAGCGGCGAGCGAGAGC640.16954540637914592No Hit
GGATTACCCGCTGAGTTTAAGCATATCAAAAAA630.16689625940447178No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA630.16689625940447178No Hit
GAAATCAACCGAGACCCCGTTAGTAGCGGCGAG600.15894881848044928No Hit
ATCCATGGATCTCCGATCGGGAAACCGTATCCA600.15894881848044928No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA600.15894881848044928No Hit
GTGAATGGGTCAAGCTAAAAAAAAAAAAAAAAA600.15894881848044928No Hit
GCTCAACACGTACAAAAAAAAAAAAAAAAAAAA590.15629967150577515No Hit
GTATCAACGCAGAGTGAATGGGTCAAGCTCAAC570.15100137755642684No Hit
TATCAACGCAGAGTGAATGGGGAATGCATTGGA570.15100137755642684No Hit
CATTGGATGGATGCCCGGGCATTGAGAAGGAAG570.15100137755642684No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA560.14835223058175268No Hit
CTCCGTGGCTAGTCTGCGCTCTTTGGACTTTGA560.14835223058175268No Hit
GTGAATGGGGCGACCCCAGGTCAGGCGGGATTA560.14835223058175268No Hit
TATCAACGCAGAGTGAATGGGGTCAAGCTCAAC550.14570308360707854No Hit
ATGGATCTCCGATCGGGAAACCGTATCCAAGCT550.14570308360707854No Hit
GTGATCCATGGATCTCCGATCGGGAAACCGTAT540.14305393663240434No Hit
GTGAATGGGTCAAGCAAAAAAAAAAAAAAAAAA540.14305393663240434No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA540.14305393663240434No Hit
CCATGGATCTCCGATCGGGAAACCGTATCCAAG530.1404047896577302No Hit
CTTTGAAAACTTAGCGAACTGAAACATCTAAGT530.1404047896577302No Hit
GTATCAACGCAGAGTGAATGGGGAATGCATTGG520.13775564268305604No Hit
CTGAAACATCTAAGTAGCTAAAGGAAGGGAAAT510.1351064957083819No Hit
AACTGAAACATCTAAGTAGCTAAAGGAAGGGAA510.1351064957083819No Hit
TATCAACGCAGAGTGAATGGGGCGACCCCAGGT490.1298082017590336No Hit
GTGAATGGGTCAAGCTCAACACGTACTCAAAAA490.1298082017590336No Hit
GTCAAGCTCAACACGTACTCAAAAAAAAAAAAA470.12450990780968527No Hit
ACGCAGAGTGAATGGGTCAAGCTCAACACGTAC470.12450990780968527No Hit
TAGTAGCGGCGAGCGAGAGCGGATTTGGGATTT460.12186076083501111No Hit
GATCCATGGATCTCCGATCGGGAAACCGTATCC460.12186076083501111No Hit
CGTCAAGCTCAACACGTACTCTGCGTTGATACC460.12186076083501111No Hit
GCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA460.12186076083501111No Hit
CGCTTTCAGAGGCGAAAGGCCATGGGGAGATAC440.11656246688566281No Hit
GGGGAGATACCGTCTGTGATCCATGGATCTCCG440.11656246688566281No Hit
CCGTATCCAAGCTCCGTGGCTAGTCTGCGCTCT430.11391331991098866No Hit
GGAAGGACGCTTTCAGAGGCGAAAGGCCATGGG430.11391331991098866No Hit
GGAATGCATTGGATGGATGCCCGGGCATTGAGA430.11391331991098866No Hit
GCTCAACACAAAAAAAAAAAAAAAAAAAAAAAA420.1112641729363145No Hit
AATCAACCGAGACCCCGTTAGTAGCGGCGAGCG420.1112641729363145No Hit
GAGTGAATGGGTCAAGCTCAAAAAAAAAAAAAA420.1112641729363145No Hit
CCCGTTAGTAGCGGCGAGCGAGAGCGGATTTGG420.1112641729363145No Hit
GCCCCAATCGGGCGGTAAATTCCGTCCAAGGCT420.1112641729363145No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA410.10861502596164035No Hit
CAACACGTACTCAAAAAAAAAAAAAAAAAAAAA410.10861502596164035No Hit
GGCTAAATACGGGCGAGAGACCGATAGCGAACA410.10861502596164035No Hit
ACCCCAGGTCAGGCGGGATTACCCGCTGAGTTT410.10861502596164035No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA400.10596587898696619No Hit
GTATCAACGCAGAGTGAATGGGTCAAGCTCAAA400.10596587898696619No Hit
GTCAAGCTCAACACAAAAAAAAAAAAAAAAAAA400.10596587898696619No Hit
GGTCAAGCTCAAAAAAAAAAAAAAAAAAAAAAA400.10596587898696619No Hit
CTGTGATCCATGGATCTCCGATCGGGAAACCGT390.10331673201229204No Hit
GGGAAACCGTATCCAAGCTCCGTGGCTAGTCTG390.10331673201229204No Hit
GTCTGTGATCCATGGATCTCCGATCGGGAAACC390.10331673201229204No Hit
GAAACATCTAAGTAGCTAAAGGAAGGGAAATCA380.10066758503761788No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGAG257.687074E-427.05
GTATCAT306.979063E-527.01
TGATACC257.687074E-427.026
TATCATC306.979063E-527.02
TCGCAGA257.687074E-427.07
ATCATCG306.979063E-527.03
ATCGCAG257.687074E-427.06
TCATCGC306.979063E-527.04
CATCGCA351.9884939E-423.1428595
ATCTACG300.002224428822.52
TCTACGC300.002224428822.53
TACGCAG300.002224428822.55
TATCTAC300.002224428822.51
CTACGCA300.002224428822.54
GATACCA350.00543597819.28571527
TTGATAC350.00543597819.28571525
TATCAAC3050.018.1475411
ATCAACG3150.016.7142872
TCAACGC3450.016.0434783
TCTGCGT5400.015.50000127
Produced by FastQC (version 0.11.2)