FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate6_4.3520000005edaf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate6_4.3520000005edaf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7980
Sequences flagged as poor quality0
Sequence length33
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCGACGTCTACGTACTCTGCGTTGATACCA1892.368421052631579No Hit
GTGAATGGGTATCGACGAAAAAAAAAAAAAAAA610.7644110275689223No Hit
GTGAATGGGTATCGACGTAAAAAAAAAAAAAAA460.5764411027568922No Hit
GTATCGACGTCAAAAAAAAAAAAAAAAAAAAAA460.5764411027568922No Hit
GTGAATGGGTATCGACGTCAAAAAAAAAAAAAA450.5639097744360901No Hit
TATCAACGCAGAGTGAATGGGTATCGACGAAAA350.43859649122807015No Hit
GTATCGACGTAAAAAAAAAAAAAAAAAAAAAAA340.4260651629072682No Hit
TATCAACGCAGAGTGAATGGGTATCGACGTCTA300.37593984962406013No Hit
TATCAACGCAGAGTGAATGGGTATCGACGTCAA300.37593984962406013No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT210.2631578947368421No Hit
GTGAATGGGGTATCGACGTCAAAAAAAAAAAAA210.2631578947368421No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA210.2631578947368421No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA200.2506265664160401No Hit
ACGCAGAGTGAATGGGTATCGACGAAAAAAAAA200.2506265664160401No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA200.2506265664160401No Hit
ACGCAGAGTGAATGGGTATCGACGTAAAAAAAA200.2506265664160401No Hit
GTGAATGGGTATCGAAAAAAAAAAAAAAAAAAA190.2380952380952381No Hit
ACGCAGAGTGAATGGGTATCGACGTCAAAAAAA190.2380952380952381No Hit
TATCAACGCAGAGTGAATGGGTATCGACGTAAA190.2380952380952381No Hit
GAGTGAATGGGTATCGACGAAAAAAAAAAAAAA180.2255639097744361No Hit
ACGCAGAGTGAATGGGTATCGACGTCTACGTAC180.2255639097744361No Hit
AACGCAGAGTGAATGGGTATCGACGTAAAAAAA180.2255639097744361No Hit
TATCAACGCAGAGTGAATGGGGTATCGACGTCA170.2130325814536341No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA160.20050125313283207No Hit
GTATCAACGCAGAGTGAATGGGTATCGACGAAA160.20050125313283207No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA160.20050125313283207No Hit
GTGAATGGGTATCGACAAAAAAAAAAAAAAAAA160.20050125313283207No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA150.18796992481203006No Hit
GTGAATGGGGTATCGACGAAAAAAAAAAAAAAA150.18796992481203006No Hit
GCATTGGATGGATGCCCGGGCATTGAGAAGGAA150.18796992481203006No Hit
TCTACGTACTCAAAAAAAAAAAAAAAAAAAAAA150.18796992481203006No Hit
GTGAATGGGGTATCGACGTAAAAAAAAAAAAAA140.17543859649122806No Hit
GTACGTAGACGTCGAAAAAAAAAAAAAAAAAAA140.17543859649122806No Hit
CGTATCGACGTCTACGTACTCTGCGTTGATACC140.17543859649122806No Hit
TATCAACGCAGAGTGAATGGGGTATCGACGTCT140.17543859649122806No Hit
GTATCAACGCAGAGTGAATGGGTATCGACGTCA140.17543859649122806No Hit
GAGTGAATGGGTATCGACGTCAAAAAAAAAAAA120.15037593984962408No Hit
GTATCGACGAAAAAAAAAAAAAAAAAAAAAAAA120.15037593984962408No Hit
AACGCAGAGTGAATGGGTATCGACGTCTACGTA120.15037593984962408No Hit
GTATCGACGTCTACGTACAAAAAAAAAAAAAAA120.15037593984962408No Hit
GTGAATGGGGTATCGACAAAAAAAAAAAAAAAA120.15037593984962408No Hit
GAGTGAATGGGTATCGAAAAAAAAAAAAAAAAA110.13784461152882205No Hit
TATCAACGCAGAGTGAATGGGGTATCGACGTAA110.13784461152882205No Hit
CCCCCCGTATCGACGTCTACGTACTCTGCGTTG110.13784461152882205No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA110.13784461152882205No Hit
GGTATCGACGTCTACGTACTCTGCGTTGATACC100.12531328320802004No Hit
GTATCGACGTCTACGTACTCAAAAAAAAAAAAA100.12531328320802004No Hit
AACGCAGAGTGAATGGGTATCGACGAAAAAAAA100.12531328320802004No Hit
ATCAACGCAGAGTGAATGGGTATCGACGTAAAA100.12531328320802004No Hit
TATCAACGCAGAGTGAATGGGGTATCGACGAAA100.12531328320802004No Hit
CGTCTACGTACTCAAAAAAAAAAAAAAAAAAAA100.12531328320802004No Hit
GTATCAACGCAGAGTGAATGGGTATCGACGTCT100.12531328320802004No Hit
ATCAACGCAGAGTGAATGGGTATCGACGAAAAA90.11278195488721805No Hit
TATCAACGCAGAGTGAATGGGGTATCGACAAAA90.11278195488721805No Hit
GTGAATGGGGTACGTAGACGTCGAAAAAAAAAA90.11278195488721805No Hit
CCCCCGTATCGACGTCTACGTACTCTGCGTTGA90.11278195488721805No Hit
ACGCAGAGTGAATGGGTATCGAAAAAAAAAAAA90.11278195488721805No Hit
ATGTATCGACGTCTACGTACTCTGCGTTGATAC80.10025062656641603No Hit
ACGCAGAGTGAATGGGGTATCGACAAAAAAAAA80.10025062656641603No Hit
GTGAATGGGTATCGACGTCTACGTACAAAAAAA80.10025062656641603No Hit
GAGTGAATGGGTATCGACGTAAAAAAAAAAAAA80.10025062656641603No Hit
GTGAATGGGTATCGACGTCTACAAAAAAAAAAA80.10025062656641603No Hit
CTCTTTGGACTTTGAAAACTTAGCGAACTGAAA80.10025062656641603No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.10025062656641603No Hit
GGCGAAAGGCCATGGGGAGATACCGTCTGTGAT80.10025062656641603No Hit
GTATCAACGCAGAGTGAATGGGGTATCGACGTC80.10025062656641603No Hit
GTATCGACGTCTACGAAAAAAAAAAAAAAAAAA80.10025062656641603No Hit
TATCAACGCAGAGTGAATGGGTATCGACAAAAA80.10025062656641603No Hit
GTATCAACGCAGAGTGAATGGGTATCGACGTAA80.10025062656641603No Hit
CCCCACGTATCGACGTCTACGTACTCTGCGTTG80.10025062656641603No Hit
GTATCGACGTCTACAAAAAAAAAAAAAAAAAAA80.10025062656641603No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACCA257.161628E-426.99999827
TGATACC257.161628E-426.99999826
TTGATAC300.00207426722.525
GTTGATA350.005073610719.28571524
CGTTGAT350.005073610719.28571523
TCTGCGT1457.4287527E-913.03448227
ATGGGGT750.001930311812.5999995
GTGAATG2350.012.0638291
TGAATGG2400.011.81252
AATGGGG1501.7682578E-711.74
CTCTGCG1801.5262049E-710.50000126
GAATGGG2701.8189894E-1210.5000013
ACTCTGC1955.238995E-69.025
CGACGTC3207.935523E-87.5937513
TCGACGT3301.3376484E-77.363636512
TACTCTG2459.328145E-57.163264824
ACGTCTA2859.303425E-67.105262815
TCTACGT2859.303425E-67.105262818
GACGTCT2859.303425E-67.105262814
GTCTACG2859.303425E-67.105262817
Produced by FastQC (version 0.11.2)