FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate6_38.3520000005f1eb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate6_38.3520000005f1eb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32408
Sequences flagged as poor quality0
Sequence length33
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTGAATGGGCAACAACGTACT3591.1077511725499878No Hit
ACAACGTACTCTGCGAAAAAAAAAAAAAAAAAA2280.7035299925944211No Hit
ACAACGTACTCTGCAAAAAAAAAAAAAAAAAAA1690.5214761787213034No Hit
GTATCAACGCAGAGTGAATGGGCAACAACGTAC1590.49061960009874106No Hit
GTTTAAGCATATCAAAAAAAAAAAAAAAAAAAA1220.37645025919526043No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCC1200.37027894347074797No Hit
CCCCAGGTCAGGCGGGATTACCCGCTGAGTTTA1160.357936312021723No Hit
ACGCAGAGTGAATGGGCAACAACGTACTCAAAA970.2993088126388546No Hit
ACAACGTACTCTGCGTAAAAAAAAAAAAAAAAA960.2962231547765984No Hit
ACAACGTACTCTGAAAAAAAAAAAAAAAAAAAA950.29313749691434215No Hit
GTGAATGGGCAACAACGTACTCAAAAAAAAAAA870.26845223401629226No Hit
CAACAACGTACTCAAAAAAAAAAAAAAAAAAAA860.265366576154036No Hit
GTTCGGCAACAACGTACTCTGCGTTGATACCAC840.25919526042952357No Hit
ACGCAGAGTGAATGGGCAACAACGTACTCTGCG790.24376697111824241No Hit
AACGTACTCTGCGAAAAAAAAAAAAAAAAAAAA770.23759565539372995No Hit
AACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA760.23450999753147372No Hit
GAGTTTAAGCATATCAAAAAAAAAAAAAAAAAA700.2159960503579363No Hit
CAACGTACTCTGCGTAAAAAAAAAAAAAAAAAA660.20365341890891137No Hit
GGTATCAACGCAGAGTGAATGGGCAACAACGTA610.18822512959763021No Hit
ATCAACGCAGAGTGAATGGGCAACAACGTACTC580.1789681560108615No Hit
GCAACAACGTACTCAAAAAAAAAAAAAAAAAAA580.1789681560108615No Hit
CAACGTACTCTGCGAAAAAAAAAAAAAAAAAAA560.17279684028634903No Hit
GGATTACCCGCTGAGTTTAAGCATATCAAAAAA550.1697111824240928No Hit
GAGTGAATGGGCAACAACGTACTCAAAAAAAAA550.1697111824240928No Hit
ACAACGTACTCAAAAAAAAAAAAAAAAAAAAAA550.1697111824240928No Hit
GTTTGGGAATGCAGCCCCAATCGGGCGGTAAAT500.15428289311281165No Hit
CAACAACGTACTCTGAAAAAAAAAAAAAAAAAA480.1481115773882992No Hit
GTGAATGGGGCGACCCCAGGTCAGGCGGGATTA480.1481115773882992No Hit
AACGCAGAGTGAATGGGCAACAACGTACTCTGC460.14194026166378673No Hit
GGCCTAAGTTCCCTGGAAAGGGGCGCCAGAGAG460.14194026166378673No Hit
ACGCAGAGTGAATGGGCAACAACGTACTCTGAA440.13576894593927427No Hit
GTGTTCGGCAACAACGTACTCTGCGTTGATACC440.13576894593927427No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGC440.13576894593927427No Hit
GCTAAATACGGGCGAGAGACCGATAGCGAACAA430.132683288077018No Hit
ACAACGTACTCTAAAAAAAAAAAAAAAAAAAAA430.132683288077018No Hit
CAACAACGTACTCTAAAAAAAAAAAAAAAAAAA420.12959763021476178No Hit
AACGCAGAGTGAATGGGCAACAACGTACTCAAA420.12959763021476178No Hit
CAACAACGTACTCTGCGAAAAAAAAAAAAAAAA420.12959763021476178No Hit
GTGAATGGGCAACAACGTACTCTGCGAAAAAAA400.12342631449024932No Hit
GGTCAGGCGGGATTACCCGCTGAGTTTAAGCAT390.12034065662799309No Hit
CAACGTACTCTGAAAAAAAAAAAAAAAAAAAAA380.11725499876573686No Hit
ACGTACTCTGCGTAAAAAAAAAAAAAAAAAAAA380.11725499876573686No Hit
TGCCCGGACCCTGTCGCACCACGAGGCGCTGTC370.11416934090348063No Hit
CAACGTACTCTGCAAAAAAAAAAAAAAAAAAAA360.1110836830412244No Hit
TATCAACGCAGAGTGAATGGGGCGACCCCAGGT360.1110836830412244No Hit
ACGCAGAGTGAATGGGCAACAACGTACTCTGCA340.10491236731671191No Hit
GTGAATGGGCAACAACGTACTCTGAAAAAAAAA330.10182670945445568No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCAC200.00848705327.00154527
GTATCAA1004.3075306E-714.850851
AGCATAT600.007341922713.5007726
ACTGTTC904.082308E-513.4799387
ATTGTTC600.00741766713.4799387
TCTGCGT5600.013.25968827
TATCAAC1552.9545845E-812.1942451
TCAACGC1802.3506072E-710.5006013
ATCAACG1802.3506072E-710.5006012
CTGTTCG3900.010.3852098
CAACGCA1904.9434857E-79.9479384
GTGTTCG1258.530565E-49.7205568
CGGCAAC7850.09.631124513
TTCGGCA7950.09.50997811
TGTTCGG7850.09.4591419
TCGGCAA8000.09.45054112
GTTCGGC8050.09.39184210
CTCTGCG8100.09.33386826
TGGGCAA2101.937824E-69.0005156
ATGGGCA2152.6664256E-68.7912015
Produced by FastQC (version 0.11.2)