FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate6_32.3520000005f121.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate6_32.3520000005f121.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33126
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTGAATGGGCTAACTATCCAC4361.3161866811567953No Hit
CTATCCACGTACTCAAAAAAAAAAAAAAAAAAA4281.292036466823643No Hit
GGCTAACTATCCACGTACTCTGCGTTGATACCA2510.7577129747026505No Hit
GTGAATGGGCTAACTATCCACAAAAAAAAAAAA2430.7335627603694983No Hit
GTATCAACGCAGAGTGAATGGGCTAACTATCCA2120.6399806798285335No Hit
ATCCACGTACTCAAAAAAAAAAAAAAAAAAAAA1810.5463985992875686No Hit
ACGCAGAGTGAATGGGCTAACTATCCACAAAAA1640.49507939382962024No Hit
TATCAACGCAGAGTGAATGGGGCTAACTATCCA1610.48602306345468815No Hit
GTGAATGGGCTAACTATCCACGTACTCAAAAAA1330.4014973132886554No Hit
GGCTAACTATCCACAAAAAAAAAAAAAAAAAAA1320.3984785364970114No Hit
ACGCAGAGTGAATGGGCTAACTATCCACGTACT1210.3652719917889271No Hit
GAGTGAATGGGCTAACTATCCACAAAAAAAAAA1000.3018776791644026No Hit
ATCCACAAAAAAAAAAAAAAAAAAAAAAAAAAA1000.3018776791644026No Hit
CTATCCACAAAAAAAAAAAAAAAAAAAAAAAAA990.2988589023727586No Hit
AACGCAGAGTGAATGGGCTAACTATCCACGTAC860.2596148040813862No Hit
AACGCAGAGTGAATGGGCTAACTATCCACAAAA830.2505584737064541No Hit
ACGCAGAGTGAATGGGGCTAACTATCCACGTAC810.2445209201231661No Hit
ACTATCCACAAAAAAAAAAAAAAAAAAAAAAAA800.2415021433315221No Hit
GGTATCAACGCAGAGTGAATGGGCTAACTATCC790.23848336653987806No Hit
GTATCAACGCAGAGTGAATGGGGCTAACTATCC760.22942703616494597No Hit
GGCTAACTATCCACGTACTCAAAAAAAAAAAAA760.22942703616494597No Hit
GCTAACTATCCACGTACTCTGCGTTGATACCAC750.22640825937330195No Hit
GTGAATGGGCTAACTAAAAAAAAAAAAAAAAAA720.21735192899836986No Hit
GTGAATGGGGCTAACTATCCACAAAAAAAAAAA720.21735192899836986No Hit
GTGAATGGGGCTAACTATCCACGTACTCAAAAA680.20527682183179374No Hit
CTATCCACGTACAAAAAAAAAAAAAAAAAAAAA660.1992392682485057No Hit
GTGAATGGGCTAACTATCCACGTACAAAAAAAA650.19622049145686168No Hit
CTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAA610.1841453842902856No Hit
CGTACTCAAAAAAAAAAAAAAAAAAAAAAAAAA570.17207027712370948No Hit
GTGAATGGGCTAACTATCCAAAAAAAAAAAAAA570.17207027712370948No Hit
ACGCAGAGTGAATGGGCTAACTATCCACGTACA560.16905150033206542No Hit
GAGTGAATGGGCTAACTATCCACGTACTCAAAA520.15697639316548934No Hit
TATCAACGCAGAGTGAATGGGCTAACTATCCAA520.15697639316548934No Hit
TCCACGTACTCAAAAAAAAAAAAAAAAAAAAAA510.1539576163738453No Hit
GAGTGAATGGGGCTAACTATCCACGTACTCAAA510.1539576163738453No Hit
ATCAACGCAGAGTGAATGGGCTAACTATCCACG490.14792006279055728No Hit
ATCAACGCAGAGTGAATGGGCTAACTATCCACA490.14792006279055728No Hit
GTGAATGGGCTAACTATAAAAAAAAAAAAAAAA480.14490128599891325No Hit
TATCTACGCAGAGTGAATGGGCTAACTATCCAC470.14188250920726922No Hit
ACTATCCACGTACTCAAAAAAAAAAAAAAAAAA460.1388637324156252No Hit
CTATCCAAAAAAAAAAAAAAAAAAAAAAAAAAA440.13282617883233713No Hit
ATCCACGTACAAAAAAAAAAAAAAAAAAAAAAA440.13282617883233713No Hit
GGCTAACTATCCACGTACAAAAAAAAAAAAAAA430.1298074020406931No Hit
GTATCATCGCAGAGTGAATGGGCTAACTATCCA420.1267886252490491No Hit
ATCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA420.1267886252490491No Hit
GAGTGAATGGGCTAACTATCCACGTACAAAAAA420.1267886252490491No Hit
AACGCAGAGTGAATGGGGCTAACTATCCACGTA410.12376984845740506No Hit
TATCAACGCAGAGTGAATGGGCTAACTATAAAA410.12376984845740506No Hit
TATCAACGCAGAGTGAATGGGCTAACTAAAAAA380.11471351808247299No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA370.11169474129082894No Hit
GTGAATGGGGCTAACTAAAAAAAAAAAAAAAAA370.11169474129082894No Hit
GAGTGAATGGGGCTAACTATCCACAAAAAAAAA370.11169474129082894No Hit
ACGCAGAGTGAATGGGCTAACTAAAAAAAAAAA360.10867596449918493No Hit
GGTATCAACGCAGAGTGAATGGGGCTAACTATC360.10867596449918493No Hit
CTAACTATCCACGTACTCAAAAAAAAAAAAAAA350.10565718770754091No Hit
CGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAA350.10565718770754091No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTACG356.326749E-627.0030272
TCTACGC356.326749E-627.0030273
TACGCAG356.326749E-627.0030275
CTACGCA356.326749E-627.0030274
GTATCAT257.662183E-427.0030251
TCATCGC257.662183E-427.0030254
CATCGCA257.662183E-427.0030255
TCGCAGA257.730414E-426.9622367
ATCGCAG356.401915E-626.9622346
AGCGGCT352.0003525E-423.1104876
TATCATC300.002217307522.5025222
ATCATCG300.002217307522.5025223
GGTATCA504.032898E-621.602421
TATCTAC509.957692E-518.9021191
TGCGTTG2650.018.34167927
GCGGCTA757.2843995E-616.177347
GTATCAA1251.4406396E-915.1216941
GATACCA550.004123279414.72892427
TATCAAC2300.014.0885351
CTGCGTT3550.014.07226
Produced by FastQC (version 0.11.2)