FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate6_30.3520000005f0df.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate6_30.3520000005f0df.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21185
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCGTCACACTACGTACTCTGCGTTGATACCA1140.5381165919282511No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA600.28321925890960586No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT590.2784989379277791No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA540.25489733301864526No Hit
GTGAATGGGTTCGTCAAAAAAAAAAAAAAAAAA450.2124144441822044No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA440.2076941232003776No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA400.18881283927307055No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA300.14160962945480293No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA290.13688930847297615No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA290.13688930847297615No Hit
GTGAATGGGGTTCGTCAAAAAAAAAAAAAAAAA290.13688930847297615No Hit
TATCAACGCAGAGTGAATGGGTTCGTCACACTA260.12272834552749586No Hit
GTGAATGGGTTCGTCACAAAAAAAAAAAAAAAA250.1180080245456691No Hit
TATCAACGCAGAGTGAATGGGTTCGTCAAAAAA240.11328770356384234No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA230.10856738258201559No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA220.1038470616001888No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTCG701.6881859E-719.245097
TCTGCGT3200.017.72342527
GCGTTGA600.007219908813.50356327
CTCTGCG4750.011.93999226
ACTCTGC5150.011.01261425
TGCGTTG1000.001447794510.80285127
TACTCTG5400.010.5027724
TGTTCGT1050.002218045510.2640498
GTACTCT5550.010.21891223
CGTTCGT2953.6379788E-1210.0465898
CGTACTC5950.09.98582822
CACACTA6000.09.90261215
CACTACG6000.09.90261217
ACGTACT6050.09.82077321
ACACTAC6050.09.82077316
TACGTAC6050.09.82077320
CTACGTA6050.09.82077319
ACTACGT6050.09.82077318
TCGTCAC6100.09.74027411
TCACACT6100.09.74027414
Produced by FastQC (version 0.11.2)