FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate6_3.3520000005ed85.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate6_3.3520000005ed85.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16204
Sequences flagged as poor quality0
Sequence length33
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCTGTTCCTCACGTACTCTGCGTTGATACCA2821.7403110343125152No Hit
CTCTGTTCCTCACGTACTCTGCGTTGATACCAC760.46901999506294745No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA390.24068131325598618No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA390.24068131325598618No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA370.22833868180696126No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA350.2159960503579363No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT340.20982473463342383No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA330.20365341890891137No Hit
GCATTGGATGGATGCCCGGGCATTGAGAAGGAA310.19131078745988644No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA280.17279684028634903No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA270.16662552456183657No Hit
GGGAGATACCGTCTGTGATCCATGGATCTCCGA230.14194026166378673No Hit
GTAGGATAGTGGCCTACCATGGTGGTAACGGGT200.12342631449024932No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA190.11725499876573686No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA190.11725499876573686No Hit
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACC180.1110836830412244No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA170.10491236731671191No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTGA1950.018.69230727
ACACTCT601.8521365E-518.07
ATACTCT603.8599956E-415.757
AGACTCT550.004006119414.7272737
ATCAACG852.2081891E-514.2941182
TCAACGC903.785691E-513.5000013
TATCAAC956.2926294E-512.7894741
TCTGCGT6850.012.416059527
AACGCAG1001.0173605E-412.155
CAACGCA1001.0173605E-412.154
TGCGTTG3350.012.08955327
CTCTGCG7800.011.24999926
ACTCTGC8150.010.93251525
CGACTCT1000.001419534710.7999997
CTGCGTT3800.010.65789526
AGAGTGA1050.002132283510.28571410
ACGTACT8800.010.27840921
CGTACTC8800.010.27840922
TCCTCAC8900.010.16292116
TCACGTA8900.010.16292119
Produced by FastQC (version 0.11.2)