FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate6_29.3520000005f0b5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate6_29.3520000005f0b5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39429
Sequences flagged as poor quality0
Sequence length33
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTTGGACCTTACGTACTCTGCGTTGATACCA4001.0144817266478987No Hit
GCTTGGACCTTACGTACTCTGCGTTGATACCAC2880.7304268431864871No Hit
GCCTTACGTACTCAAAAAAAAAAAAAAAAAAAA2580.6543407136878947No Hit
GTACGTAAGGTCCAAAAAAAAAAAAAAAAAAAA1650.41847371224225827No Hit
TATCAACGCAGAGTGAATGGGCCTTACGTACTC1640.4159375079256385No Hit
GAGTACGTAAGGTCCAAAAAAAAAAAAAAAAAA1640.4159375079256385No Hit
TACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA1480.3753582388597225No Hit
GACCTTACGTACTCAAAAAAAAAAAAAAAAAAA1100.2789824748281722No Hit
GTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA960.2434756143954957No Hit
GTATCAACGCAGAGTGAATGGGCCTTACGTACT940.2384032057622562No Hit
GGCCTTACGTACTCAAAAAAAAAAAAAAAAAAA880.22318597986253774No Hit
TATCAACGCAGAGTGAATGGGACCTTACGTACT850.21557736691267848No Hit
GTGAATGGGACCTTACGTACTCAAAAAAAAAAA820.20796875396281925No Hit
GTGAATGGGCCTTACGTACTCAAAAAAAAAAAA750.190215323746481No Hit
ACCTTACGTACTCAAAAAAAAAAAAAAAAAAAA720.18260671079662177No Hit
GTGAATGGGAGGTCCAAAAAAAAAAAAAAAAAA720.18260671079662177No Hit
ACGCAGAGTGAATGGGCCTTACGTACTCAAAAA710.18007050648000203No Hit
GTGAATGGGGAGTACGTAAGGTCCAAAAAAAAA700.17753430216338228No Hit
CCTTACGTACTCAAAAAAAAAAAAAAAAAAAAA690.17499809784676254No Hit
GGTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA620.1572446676304243No Hit
GCTTGGACCAAAAAAAAAAAAAAAAAAAAAAAA610.15470846331380456No Hit
GTGAATGGGTACGTAAGGTCCAAAAAAAAAAAA550.1394912374140861No Hit
GTGAATGGGGGTCCAAAAAAAAAAAAAAAAAAA540.13695503309746632No Hit
GAGTGAATGGGGTCCAAAAAAAAAAAAAAAAAA530.1344188287808466No Hit
GAGTGAATGGGCCTTACGTACTCAAAAAAAAAA510.12934642014760708No Hit
GTGAATGGGAGTACGTAAGGTCCAAAAAAAAAA510.12934642014760708No Hit
TACGTACTCTGCGTTGATACAAAAAAAAAAAAA510.12934642014760708No Hit
GAATGGGACCTTACGTACTCAAAAAAAAAAAAA490.12427401151436761No Hit
TGCTTGGACCAAAAAAAAAAAAAAAAAAAAAAA490.12427401151436761No Hit
CTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA480.12173780719774785No Hit
GGGTACGTAAGGTCCAAAAAAAAAAAAAAAAAA480.12173780719774785No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA450.11412919424788862No Hit
GGTCCAAGCAAAAAAAAAAAAAAAAAAAAAAAA450.11412919424788862No Hit
TACGTACTCTGCGTTGATACCAAAAAAAAAAAA420.10652058129802935No Hit
GTATCAACGCAGAGTGAATGGGACCTTACGTAC410.10398437698140962No Hit
ACGCAGAGTGAATGGGCCTTACGTACTCTGCGT410.10398437698140962No Hit
ACGTAAGGTCCAAAAAAAAAAAAAAAAAAAAAA400.10144817266478988No Hit
GTATCATCGCAGAGTGAATGGGCCTTACGTACT400.10144817266478988No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAT306.9426256E-527.0254121
ACCCTGC200.00852527626.9911176
CAACTCA300.002214126522.5211771
ATACCAC751.623448E-819.81863627
GGTATCA559.210555E-619.6548461
AACTCAG350.00545088719.279372
ACTCAGA350.00545088719.279373
TCAGAGT350.00545088719.279375
CTCAGAG350.00545088719.279374
TATCATC450.00108697517.9940782
GTTGCTT450.00108697517.9940787
TATCAAC3450.016.8419251
GTATCAA1450.016.7743951
ATCAACG3550.016.7268892
TCAACGC3550.016.3467333
CAACGCA3550.015.9665774
ATCATCG550.004154297514.7224273
TCATCGC550.004154297514.7224274
CATCGCA550.004154297514.7224275
AGTGCTT751.3314397E-414.3952637
Produced by FastQC (version 0.11.2)