FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate6_28.3520000005f09c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate6_28.3520000005f09c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15303
Sequences flagged as poor quality0
Sequence length33
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTCCTCTACGTACTCTGCGTTGATACCA2171.4180226099457622No Hit
CTCGTCCTCTACGTACTCTGCGTTGATACCACT830.5423773116382409No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT670.43782264915376073No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA580.3790106515062406No Hit
GTGAATGGGATCTCGTCCAAAAAAAAAAAAAAA570.3724759851009606No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA520.3398026530745606No Hit
TATCAACGCAGAGTGAATGGGATCTCGTCCAAA500.32673332026400054No Hit
ACGCAGAGTGAATGGGATCTCGTCCAAAAAAAA460.3005946546428805No Hit
GAGTGAATGGGATCTCGTCCAAAAAAAAAAAAA450.2940599882376005No Hit
CATCTCGTCCTCTACGTACTCTGCGTTGATACC450.2940599882376005No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA440.28752532183232044No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA440.28752532183232044No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA400.26138665621120044No Hit
GTAGAGGACGAAAAAAAAAAAAAAAAAAAAAAA340.22217865777952034No Hit
GTGAATGGGATCTCGTCAAAAAAAAAAAAAAAA340.22217865777952034No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA320.20910932496896031No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA310.20257465856368032No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA290.1895053257531203No Hit
GCATTGGATGGATGCCCGGGCATTGAGAAGGAA270.17643599294256027No Hit
TATCAACGCAGAGTGAATGGGATCTCGTCCTCT250.16336666013200027No Hit
TATCAACGCAGAGTGAATGGGATCTCGTCAAAA240.15683199372672024No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA230.15029732732144024No Hit
GTATCAACGCAGAGTGAATGGGATCTCGTCCTC230.15029732732144024No Hit
GGTATCAACGCAGAGTGAATGGGAAGCAGTGGT210.13722799451088022No Hit
TATCAACGCAGAGTGAATGGGATCTCGTCCTCA210.13722799451088022No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA200.13069332810560022No Hit
GAGTGAATGGGATCTCGTCCTCAAAAAAAAAAA190.12415866170032021No Hit
GTGAATGGGATCTCGTCCTCAAAAAAAAAAAAA190.12415866170032021No Hit
GTCCTCTACGTACTCAAAAAAAAAAAAAAAAAA190.12415866170032021No Hit
GTATCAACGCAGAGTGAATGGGATCTCGTCCAA180.1176239952950402No Hit
GGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAA180.1176239952950402No Hit
GCCATGGGGAGATACCGTCTGTGATCCATGGAT180.1176239952950402No Hit
CCCCCATCTCGTCCTCTACGTACTCTGCGTTGA170.11108932888976017No Hit
GTGAATGGGGTACGTAGAGGACGAAAAAAAAAA170.11108932888976017No Hit
GTGAATGGGGATCTCGTCCAAAAAAAAAAAAAA170.11108932888976017No Hit
GATCTCGTCCAAAAAAAAAAAAAAAAAAAAAAA170.11108932888976017No Hit
CTCTTTGGACTTTGAAAACTTAGCGAACTGAAA160.10455466248448016No Hit
GTGAATGGGGATCTCGAAAAAAAAAAAAAAAAA160.10455466248448016No Hit
CCCCTATCTCGTCCTCTACGTACTCTGCGTTGA160.10455466248448016No Hit
ACGCAGAGTGAATGGGATCTCGTCCTCAAAAAA160.10455466248448016No Hit
ATCTCGTCCAAAAAAAAAAAAAAAAAAAAAAAA160.10455466248448016No Hit
GTGAATGGGGATCTCGTCAAAAAAAAAAAAAAA160.10455466248448016No Hit
CATCTAAGTAGCTAAAAAAAAAAAAAAAAAAAA160.10455466248448016No Hit
GGGAGATACCGTCTGTGATCCATGGATCTCCGA160.10455466248448016No Hit
GAGTGAATGGGATCTCGTCAAAAAAAAAAAAAA160.10455466248448016No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTGA1950.018.69230827
TATCAAC1501.0531949E-913.4999991
ATCAACG1654.3801265E-912.2727272
TGCGTTG3100.011.75806426
TCAACGC1751.051194E-811.5714293
CAACGCA1801.5963451E-811.2500014
AACGCAG2007.548442E-810.1255
CTGCGTT3800.09.59210525
TATCTCG1607.393265E-59.281258
GTATCAA1350.00161845769.01
CCATCTC1350.00161845769.04
AGAGTGA2101.6911003E-58.35714210
CAGAGTG2152.2747876E-58.162799
ACGCAGA2501.927605E-68.16
GTGAATG4001.8189894E-118.11
TCTGCGT5400.08.027
TGAATGG3909.276846E-117.9615392
CGCAGAG2401.0155572E-57.8757
GCAGAGT2254.0250074E-57.88
CTCTGCG5700.07.57894726
Produced by FastQC (version 0.11.2)