FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate6_25.3520000005f03f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate6_25.3520000005f03f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15962
Sequences flagged as poor quality0
Sequence length33
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCC600.3758927452700163No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGC430.269389800776845No Hit
CAACGAACCAAGACGTACTCTGCGTTGATACCA330.20674100989850897No Hit
CCCCAGGTCAGGCGGGATTACCCGCTGAGTTTA320.20047613081067536No Hit
GGCTAAATACGGGCGAGAGACCGATAGCGAACA310.19421125172284173No Hit
GCTAAATACGGGCGAGAGACCGATAGCGAACAA280.17541661445934095No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA260.16288685628367372No Hit
GGCCTAAGTTCCCTGGAAAGGGGCGCCAGAGAG240.15035709810800651No Hit
GTCCTAAGATGAGCTCAACGAGAACAGAAATCT200.1252975817566721No Hit
AACCAAGACGTACTCAAAAAAAAAAAAAAAAAA200.1252975817566721No Hit
GTTTGGGAATGCAGCCCCAATCGGGCGGTAAAT190.11903270266883849No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCT190.11903270266883849No Hit
GTCCAAGGCTAAATACGGGCGAGAGACCGATAG180.11276782358100489No Hit
GCCCCAATCGGGCGGTAAATTCCGTCCAAGGCT180.11276782358100489No Hit
GGATTACCCGCTGAGTTTAAGCATATCAAAAAA170.10650294449317128No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA160.10023806540533768No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGTTG500.002119746516.227
TCTGCGT1203.067269E-612.37527
CTCTGCG1452.5219548E-510.2413826
ACTCTGC1503.6658115E-59.90000125
TACTCTG1659.402036E-69.81818224
GTACTCT1607.4477895E-59.2812523
ACGTACT1701.4437568E-48.73529421
GACGTAC1751.9784545E-48.48571420
CGTACTC1751.9784545E-48.48571422
AACGAAC1956.770925E-58.30769210
CAAGACG1853.6096078E-48.02702717
AAGACGT1853.6096078E-48.02702718
AGACGTA1853.6096078E-48.02702719
ACCAAGA1904.8107168E-47.815789715
ACGAACC1904.8107168E-47.815789711
AACCAAG1956.3584535E-47.61538414
CGAACCA1956.3584535E-47.61538412
CCAAGAC1956.3584535E-47.61538416
CAACGAA2152.1123652E-47.53488359
GAACCAA2050.0010852427.24390213
Produced by FastQC (version 0.11.2)