FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate6_23.3520000005effd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate6_23.3520000005effd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33608
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGATACGTACTCAAAAAAAAAAAAAAAAAAA5901.7555343965722447No Hit
GATACGTACTCAAAAAAAAAAAAAAAAAAAAAA4041.2020947393477743No Hit
TATCAACGCAGAGTGAATGGGATAGCAGATACG3411.0146393715781956No Hit
GTGAATGGGATAGCAGATACGTACTCAAAAAAA3391.0086884075220186No Hit
GTGAATGGGATAGCAGATACGTACAAAAAAAAA1980.5891454415615329No Hit
GCAGATACGTACAAAAAAAAAAAAAAAAAAAAA1460.4344203761009284No Hit
GTACTCAAAAAAAAAAAAAAAAAAAAAAAAAAA1440.42846941204475125No Hit
GGATAGCAGATACGTACTCTGCGTTGATACCAC1350.40169007379195426No Hit
GGATAGCAGATACGTACTCAAAAAAAAAAAAAA1340.3987145917638658No Hit
ACGCAGAGTGAATGGGATAGCAGATACGTACTC1320.39276362770768863No Hit
GTATCAACGCAGAGTGAATGGGATAGCAGATAC1170.3481313972863604No Hit
GAGTGAATGGGATAGCAGATACGTACTCAAAAA1070.3183765770054749No Hit
GCAGATACGTACTAAAAAAAAAAAAAAAAAAAA1060.31540109497738633No Hit
GATACGTACAAAAAAAAAAAAAAAAAAAAAAAA1060.31540109497738633No Hit
GTGAATGGGATAGCAGATACGTACTAAAAAAAA1030.3064746488931207No Hit
AACGCAGAGTGAATGGGATAGCAGATACGTACT960.28564627469650083No Hit
TATCTACGCAGAGTGAATGGGATAGCAGATACG950.28267079266841233No Hit
TATCAACGCAGAGTGAATGGGTAGCAGATACGT940.2796953106403237No Hit
GGATAGCAGATACGTACAAAAAAAAAAAAAAAA800.238038562247084No Hit
TACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA790.2350630802189955No Hit
GTATCATCGCAGAGTGAATGGGATAGCAGATAC780.23208759819090694No Hit
ACGCAGAGTGAATGGGATAGCAGATACGTACAA760.22613663413472984No Hit
GATACGTACTAAAAAAAAAAAAAAAAAAAAAAA720.21423470602237563No Hit
ATCAACGCAGAGTGAATGGGATAGCAGATACGT670.1993572958819329No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA660.19638181385384432No Hit
GGTATCAACGCAGAGTGAATGGGATAGCAGATA620.1844798857414901No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCC570.16960247560104735No Hit
GTATCAACGCAGAGTGAATGGGTAGCAGATACG560.16662699357295882No Hit
GATAGCAGATACGTACTCTGCGTTGATACCACT550.1636515115448703No Hit
GAATGGGATAGCAGATACGTACTCAAAAAAAAA530.15770054748869317No Hit
ACGCAGAGTGAATGGGTAGCAGATACGTACTCA520.15472506546060463No Hit
GAGTGAATGGGATAGCAGATACGTACAAAAAAA510.15174958343251607No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA500.1487741014044275No Hit
CAACGCAGAGTGAATGGGATAGCAGATACGTAC490.14579861937633898No Hit
CAACTCAGAGTGAATGGGATAGCAGATACGTAC490.14579861937633898No Hit
CAGATACGTACTCAAAAAAAAAAAAAAAAAAAA460.13687217329207332No Hit
GGATAGCAGATACGTACTAAAAAAAAAAAAAAA450.13389669126398476No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT440.13092120923589623No Hit
GATACGTACTCTAAAAAAAAAAAAAAAAAAAAA440.13092120923589623No Hit
GGATAGCAGAAAAAAAAAAAAAAAAAAAAAAAA430.12794572720780767No Hit
AACGCAGAGTGAATGGGATAGCAGATACGTACA420.12497024517971911No Hit
CGTACTCAAAAAAAAAAAAAAAAAAAAAAAAAA420.12497024517971911No Hit
GTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA420.12497024517971911No Hit
GAATGGGATAGCAGATACGTACAAAAAAAAAAA420.12497024517971911No Hit
GTGAATGGGTAGCAGATACGTACTCAAAAAAAA410.12199476315163058No Hit
TAGCAGATACGTACAAAAAAAAAAAAAAAAAAA400.119019281123542No Hit
GTGAATGGGATAGCAGAAAAAAAAAAAAAAAAA390.11604379909545347No Hit
ACGCAGAGTGAATGGGATAGCAGATACGTACTA370.11009283503927636No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA340.1011663889550107No Hit
CGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAA340.1011663889550107No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACTCA405.798647E-726.9955371
TATCATC750.026.9955352
GTATCAT800.025.3083151
ATCATCG800.025.3083153
TCATCGC800.025.3083154
CATCGCA800.025.3083155
ATCTACG501.4743819E-724.295982
TCTACGC501.4743819E-724.295983
TACGCAG501.4743819E-724.295985
TATCTAC501.4743819E-724.295981
CTACGCA501.4743819E-724.295984
GAGTAAA300.002221652822.496281
AGTAAAT300.002221652822.496282
TCGCAGA900.022.4962797
ATCGCAG1000.021.5964286
TCAGAGT454.4399687E-520.9965275
AACTCAG559.232379E-619.6331182
ACTCAGA559.232379E-619.6331183
CTCAGAG559.232379E-619.6331184
TAAATGG350.005429324719.2825264
Produced by FastQC (version 0.11.2)