FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate6_22.3520000005efe0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate6_22.3520000005efe0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38706
Sequences flagged as poor quality0
Sequence length33
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTGAATGGGTAGTTCTGACAC3180.8215780499147418No Hit
GTGAATGGGTAGTTCTGACACAAAAAAAAAAAA2410.6226424843693484No Hit
GTTCTGACACGTACAAAAAAAAAAAAAAAAAAA2370.6123081692760812No Hit
GTATCAACGCAGAGTGAATGGGTAGTTCTGACA1740.449542706557123No Hit
GGGTAGTTCTGACACGTACTCTGCGTTGATACC1600.41337260373068774No Hit
ACGCAGAGTGAATGGGTAGTTCTGACACAAAAA1380.3565338707177182No Hit
CGGTAGTTCTGACACGTACTCTGCGTTGATACC1350.34878313439776776No Hit
GTTCTGACACGTACTCAAAAAAAAAAAAAAAAA1260.3255309254379166No Hit
GTGAATGGGGTAGTTCAAAAAAAAAAAAAAAAA1220.3151966103446494No Hit
TATCAACGCAGAGTGAATGGGGTAGTTCTGACA1170.3022787164780654No Hit
GTTCTGACACAAAAAAAAAAAAAAAAAAAAAAA1050.27127577119826385No Hit
TATCAACGCAGAGTGAATGGGGTAGTTCAAAAA920.23768924714514544No Hit
GTGAATGGGTAGTTCAAAAAAAAAAAAAAAAAA830.21443703818529425No Hit
AACGCAGAGTGAATGGGTAGTTCTGACACAAAA750.19376840799875988No Hit
GTATCATCGCAGAGTGAATGGGTAGTTCTGACA750.19376840799875988No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA690.1782669353588591No Hit
GGTATCAACGCAGAGTGAATGGGTAGTTCTGAC670.1730997778122255No Hit
GTGAATGGGGTAGTTCTGACACAAAAAAAAAAA650.16793262026559191No Hit
TATCTACGCAGAGTGAATGGGTAGTTCTGACAC610.1575983051723247No Hit
GGGTAGTTCTGACACAAAAAAAAAAAAAAAAAA550.14209683253242392No Hit
GTGAATGGGTAGTTCTGACACGTACAAAAAAAA550.14209683253242392No Hit
CGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAA540.13951325375910711No Hit
GAGTGAATGGGTAGTTCTGACACAAAAAAAAAA530.1369296749857903No Hit
TTCTGACACAAAAAAAAAAAAAAAAAAAAAAAA520.1343460962124735No Hit
GACACGTACTCAAAAAAAAAAAAAAAAAAAAAA500.12917893866583993No Hit
GTATCAACGCAGAGTGAATGGGGTAGTTCTGAC470.12142820234588954No Hit
ACGCAGAGTGAATGGGGTAGTTCAAAAAAAAAA470.12142820234588954No Hit
GAGTGAATGGGGTAGTTCAAAAAAAAAAAAAAA440.11367746602593913No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCC430.11109388725262233No Hit
ACGCAGAGTGAATGGGGTAGTTCTGACACGTAC430.11109388725262233No Hit
AACGCAGAGTGAATGGGTAGTTCTGACACGTAC420.10851030847930554No Hit
CGTACTCAAAAAAAAAAAAAAAAAAAAAAAAAA420.10851030847930554No Hit
TATCAACGCAGAGTGAATGGGTAGTTCAAAAAA420.10851030847930554No Hit
GTTCTGACACGTAAAAAAAAAAAAAAAAAAAAA400.10334315093267193No Hit
GTGAATGGGGTAGTTCTGACACGTACAAAAAAA400.10334315093267193No Hit
AGGTAGTTCTGACACGTACTCTGCGTTGATACC400.10334315093267193No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTAC307.0068774E-526.9870621
TCGCAGA200.00852954426.9870627
TGTGGTA257.71257E-426.9870626
ATCGCAG200.00852954426.9870626
TGCGGTA200.00852954426.9870626
CATCGCA200.00852954426.9870625
TGATACC756.8030204E-1021.61761727
TTGATAC801.5224941E-920.26651626
ATCTACG404.923589E-420.2402972
TCTACGC404.923589E-420.2402973
TACGCAG350.005453772419.2764745
GTTGATA853.2396201E-919.07436825
CTACGCA450.001087558817.9913774
GTATCAA1350.016.9918561
TATCAAC2800.016.8669151
TGCGTTG3700.016.79747227
GCAGAGA500.002202243816.1922388
TTGGTAG500.002202243816.1922387
ACGGTAG757.284667E-616.1922387
GGTATCA500.002202243816.1922381
Produced by FastQC (version 0.11.2)