FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate6_21.3520000005efc6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate6_21.3520000005efc6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21724
Sequences flagged as poor quality0
Sequence length33
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCAAGGTAAAAAAAAAAAAAAAAAAAAAAAA810.37285951021911246No Hit
GTTCAAGGTACGTACTCAAAAAAAAAAAAAAAA700.322224268090591No Hit
GTTCAAGGAAAAAAAAAAAAAAAAAAAAAAAAA620.2853986374516664No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT550.25317621064260726No Hit
GGTACGTACTCTGCGTTGATACAAAAAAAAAAA500.23016019149327932No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA470.21635058000368254No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA460.21174737617381698No Hit
GTACGTACTCTGCGTTGATACAAAAAAAAAAAA450.20714417234395138No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA420.19333456085435463No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA410.18873135702448904No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA400.18412815319462347No Hit
CCCCAGTTCAAGGTACGTACTCTGCGTTGATAC370.1703185417050267No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA370.1703185417050267No Hit
TATCAACGCAGAGTGAATGGGCCAGTTCAAGGT360.16571533787516113No Hit
ACTCTGCGTTGATACAAAAAAAAAAAAAAAAAA360.16571533787516113No Hit
TATCAACGCAGAGTGAATGGGTACGTACTCTGC330.15190572638556435No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA300.1380961148959676No Hit
GTGAATGGGTACGTACTCTGCGTTGATACAAAA300.1380961148959676No Hit
GCGTTGATACAAAAAAAAAAAAAAAAAAAAAAA290.133492911066102No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA290.133492911066102No Hit
TACGTACTCTGCGTTGATACAAAAAAAAAAAAA250.11508009574663966No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA230.10587368808690849No Hit
GTGAATGGGCCAGTTCAAGGTAAAAAAAAAAAA230.10587368808690849No Hit
CTCCAGTTCAAGGTACGTACTCTGCGTTGATAC230.10587368808690849No Hit
ACGCAGAGTGAATGGGTACGTACTCTGCGTTGA220.1012704842570429No Hit
GCCCAGTTCAAGGTACGTACTCTGCGTTGATAC220.1012704842570429No Hit
ACGCAGAGTGAATGGGCCAGTTCAAGGTAAAAA220.1012704842570429No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCCAG200.00842835827.06
CCTCCAG200.00842835827.06
CGCCCAG501.421813E-724.36
ACCCCAG300.002194170422.4999986
AGCCAGT503.9306433E-621.5999987
GCCCAGT952.7284841E-1119.8947377
GTCCAGT350.005362991219.2857157
TACCCAG350.005362991219.2857156
TCTGCGT10000.018.63000127
GTCCCAG450.001063154418.0000026
TCCCAGT1001.1659722E-917.557
TATCAAC1002.276829E-816.1999991
TCCAGTT2400.015.7499998
CTCTGCG11950.015.58995726
CCCAGTT5050.015.2376238
AAGGTAC12550.014.84462116
AGGTACG12400.014.80645317
TCAACGC1107.353992E-814.7272733
ATCAACG1107.353992E-814.7272732
ACTCTGC12750.014.61176625
Produced by FastQC (version 0.11.2)