FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate6_20.3520000005efad.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate6_20.3520000005efad.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30307
Sequences flagged as poor quality0
Sequence length33
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTAGGAGACTACGTACTCTGCGTTGATACCA2930.9667733526907976No Hit
GTAGGAGACTACGTACTCTGCGTTGATACCACT2820.9304781073679347No Hit
ACGTAGGAGAAAAAAAAAAAAAAAAAAAAAAAA1720.5675256541393077No Hit
ACGTAGGAGACAAAAAAAAAAAAAAAAAAAAAA1640.5411291120863168No Hit
GTGAATGGGACGTAGGAGACAAAAAAAAAAAAA1320.43554294387435244No Hit
GGACGTAGGAGACAAAAAAAAAAAAAAAAAAAA1020.336555911175636No Hit
GGAGACAAAAAAAAAAAAAAAAAAAAAAAAAAA990.32665720790576436No Hit
GTGAATGGGACGTAGGAGAAAAAAAAAAAAAAA840.27716369155640613No Hit
TATCAACGCAGAGTGAATGGGACGTAGGAGACA840.27716369155640613No Hit
ACGCAGAGTGAATGGGACGTAGGAGACAAAAAA800.26396542052991057No Hit
GTAGGAGACAAAAAAAAAAAAAAAAAAAAAAAA760.25076714950341505No Hit
TATCAACGCAGAGTGAATGGGACGTAGGAGAAA750.24746758174679118No Hit
GGACGTAGGAGAAAAAAAAAAAAAAAAAAAAAA620.2045732009106807No Hit
CGTAGGAGACTACGTACTCTGCGTTGATACCAC610.20127363315405683No Hit
GAATGGGACGTAGGAGACAAAAAAAAAAAAAAA610.20127363315405683No Hit
ACGCAGAGTGAATGGGACGTAGGAGAAAAAAAA610.20127363315405683No Hit
GGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA540.17817665885768963No Hit
GAGTGAATGGGACGTAGGAGAAAAAAAAAAAAA500.16497838783119412No Hit
GAGTGAATGGGACGTAGGAGACAAAAAAAAAAA470.15507968456132248No Hit
GTATCAACGCAGAGTGAATGGGACGTAGGAGAC450.1484805490480747No Hit
GACGTAGGAGACAAAAAAAAAAAAAAAAAAAAA450.1484805490480747No Hit
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA430.14188141353482692No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA420.13858184577820307No Hit
GAATGGGACGTAGGAGAAAAAAAAAAAAAAAAA410.1352822780215792No Hit
GTGAATGGGGACGTAGGAGAAAAAAAAAAAAAA390.1286831425083314No Hit
GAACGTAGGAGACAAAAAAAAAAAAAAAAAAAA370.12208400699508365No Hit
GTGAATGGGACGTAGGAAAAAAAAAAAAAAAAA360.11878443923845977No Hit
GGTATCAACGCAGAGTGAATGGGACGTAGGAGA350.11548487148183588No Hit
AACGCAGAGTGAATGGGACGTAGGAGACAAAAA350.11548487148183588No Hit
GAACGTAGGAGAAAAAAAAAAAAAAAAAAAAAA350.11548487148183588No Hit
CCCCCACGTAGGAGACTACGTACTCTGCGTTGA340.11218530372521199No Hit
GTATCAACGCAGAGTGAATGGGACGTAGGAGAA330.10888573596858811No Hit
GTGAATGGGAACGTAGGAGAAAAAAAAAAAAAA320.10558616821196425No Hit
GTGAATGGGGACGTAGGAGACAAAAAAAAAAAA310.10228660045534035No Hit
GTGAATGGGGACGTAGGAAAAAAAAAAAAAAAA310.10228660045534035No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACTCA554.4383341E-1027.01
GTATAAC200.00848104627.01
TACCACT257.6523185E-427.027
ATCTACG451.6086196E-624.02
GTATCAT451.6086196E-624.01
TCTACGC451.6086196E-624.03
TATCTAC451.6086196E-624.01
TATCATC401.7776594E-523.6252
TCGCAGA401.7776594E-523.6257
ATCATCG401.7776594E-523.6253
ATCGCAG401.7776594E-523.6256
AACTCAG603.3580363E-822.52
CTCAGAG603.3580363E-822.54
TCAGAGT553.668829E-722.090915
TACGCAG504.018353E-621.65
CTACGCA504.018353E-621.64
TCATCGC454.415735E-521.04
CATCGCA454.415735E-521.05
ACTCAGA657.834569E-820.769233
ATACCAC350.005412034719.28571526
Produced by FastQC (version 0.11.2)