FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate6_2.3520000005ed6b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate6_2.3520000005ed6b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49868
Sequences flagged as poor quality0
Sequence length33
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCC1860.37298467955402265No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGC950.1905029277292051No Hit
GCTAAATACGGGCGAGAGACCGATAGCGAACAA800.16042351808775165No Hit
GTTTGGGAATGCAGCCCCAATCGGGCGGTAAAT750.15039704820726718No Hit
GGCCTAAGTTCCCTGGAAAGGGGCGCCAGAGAG690.13836528435068582No Hit
GGCTAAATACGGGCGAGAGACCGATAGCGAACA680.13635999037458893No Hit
CCCTAGCTCACTACCACGTACTCTGCGTTGATA660.13234940242239512No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA650.1303441084462982No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT520.10427528675703858No Hit
CCCCAGGTCAGGCGGGATTACCCGCTGAGTTTA500.10026469880484477No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGACA200.00853281327.04
TTCTAGC200.00853281327.04
AATGGGT404.93516E-420.254
GTTGATA951.3253157E-817.05263127
TATCAAC1154.638423E-1016.4347821
TCAACGC1258.54925E-1116.23
ATCAACG1208.4764906E-1015.7500012
CAACGCA1404.7475623E-1014.4642844
CATAGCT853.8675225E-412.7058837
GCGTTGA2151.8189894E-1212.5581427
CGTTGAT1359.743853E-712.026
TGCGTTG3750.011.87999927
AACGCAG1604.832509E-811.81255
ACTAGCT800.003568404111.81258
TCTGCGT7300.011.46575427
GCTAGCT1000.001513134910.88
CTGCGTT4150.010.73493926
CAGAGTG1701.3599984E-610.3235299
AGAGTGA1701.3599984E-610.32352910
GCAGAGT1701.3599984E-610.3235298
Produced by FastQC (version 0.11.2)