FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate6_19.3520000005ef83.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate6_19.3520000005ef83.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30968
Sequences flagged as poor quality0
Sequence length33
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTGAATGGGCTCTGAACGTAC3070.9913459054507878No Hit
GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2330.7523895634203048No Hit
GTATCAACGCAGAGTGAATGGGCTCTGAACGTA1930.6232239731335573No Hit
GTGAATGGGCTCTGAACAAAAAAAAAAAAAAAA1820.5877034358047016No Hit
GCTCTGAACGTACTCAAAAAAAAAAAAAAAAAA1750.5650994575045208No Hit
TATCAACGCAGAGTGAATGGGCTCTGAACAAAA1690.5457246189615086No Hit
ACGCAGAGTGAATGGGCTCTGAACAAAAAAAAA1530.49405838284680964No Hit
GCTCTGAACAAAAAAAAAAAAAAAAAAAAAAAA1500.4843709635753035No Hit
GTATCATCGCAGAGTGAATGGGCTCTGAACGTA1420.458537845517954No Hit
GTGAATGGGCTCTGAAAAAAAAAAAAAAAAAAA1340.4327047274606045No Hit
TATCAACGCAGAGTGAATGGGCTCTGAACGAAA1300.41978816843192973No Hit
TATCAACGCAGAGTGAATGGGCTCTGAAAAAAA1170.3778093515887368No Hit
GTGAATGGGCTCTGAACGTACTCAAAAAAAAAA1070.3455179540170499No Hit
ACGCAGAGTGAATGGGCTCTGAACGAAAAAAAA1060.34228881425988117No Hit
GTGAATGGGCTCTGAACGAAAAAAAAAAAAAAA1050.33905967450271246No Hit
GAGTGAATGGGCTCTGAACAAAAAAAAAAAAAA1050.33905967450271246No Hit
ACGCAGAGTGAATGGGCTCTGAACGTACTCTGC930.30030999741668823No Hit
GAACGTACTCTGCGTAAAAAAAAAAAAAAAAAA910.2938517179023508No Hit
ACGCAGAGTGAATGGGCTCTGAAAAAAAAAAAA880.28416429863084475No Hit
GAACGTACTCAAAAAAAAAAAAAAAAAAAAAAA880.28416429863084475No Hit
GTATCAACGCAGAGTGAATGGGCTCTGAACAAA870.28093515887367604No Hit
ACGCAGAGTGAATGGGCTCTGAACGTACTCAAA850.2744768793593387No Hit
GAGTGAATGGGCTCTGAACGAAAAAAAAAAAAA850.2744768793593387No Hit
AACGCAGAGTGAATGGGCTCTGAACAAAAAAAA820.2647894600878326No Hit
TATCTACGCAGAGTGAATGGGCTCTGAACGTAC790.25510204081632654No Hit
CTCTGAACGTACTCAAAAAAAAAAAAAAAAAAA790.25510204081632654No Hit
GCTCTGAACGTACAAAAAAAAAAAAAAAAAAAA740.23895634203048308No Hit
GCTCTGAACGAAAAAAAAAAAAAAAAAAAAAAA720.2324980625161457No Hit
GGTATCAACGCAGAGTGAATGGGCTCTGAACGT650.20989408421596487No Hit
GTATCAACGCAGAGTGAATGGGCTCTGAACGAA620.20020666494445877No Hit
GTGAATGGGCTCTGAACGTACAAAAAAAAAAAA620.20020666494445877No Hit
GAGTGAATGGGCTCTGAAAAAAAAAAAAAAAAA610.19697752518729011No Hit
GTATCATCGCAGAGTGAATGGGCTCTGAACAAA610.19697752518729011No Hit
GAGTGAATGGGCTCTGAACGTACTCAAAAAAAA590.19051924567295273No Hit
AACGCAGAGTGAATGGGCTCTGAACGAAAAAAA530.17114440712994058No Hit
ATCAACGCAGAGTGAATGGGCTCTGAACGTACT520.1679152673727719No Hit
TATCTACGCAGAGTGAATGGGCTCTGAACAAAA520.1679152673727719No Hit
AACGCAGAGTGAATGGGCTCTGAACGTACTCTG510.1646861276156032No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA500.16145698785843451No Hit
CCCCCAATGCGCTCTGAACGTACTCTGCGTTGA490.15822784810126583No Hit
GTATCAACGCAGAGTGAATGGGCTCTGAAAAAA450.14531128907259105No Hit
AACGCAGAGTGAATGGGCTCTGAAAAAAAAAAA440.14208214931542237No Hit
AACGCAGAGTGAATGGGCTCTGAACGTACTCAA440.14208214931542237No Hit
ACGCAGAGTGAATGGGCTCTGAACGTACAAAAA440.14208214931542237No Hit
GAACGTACTCTGCGAAAAAAAAAAAAAAAAAAA440.14208214931542237No Hit
GAACGTACAAAAAAAAAAAAAAAAAAAAAAAAA440.14208214931542237No Hit
GAACGAAAAAAAAAAAAAAAAAAAAAAAAAAAA430.1388530095582537No Hit
GTGAATGGGAATGCGCTCAAAAAAAAAAAAAAA390.1259364505295789No Hit
GTATCATCGCAGAGTGAATGGGCTCTGAACGAA390.1259364505295789No Hit
CGTACTCAAAAAAAAAAAAAAAAAAAAAAAAAA390.1259364505295789No Hit
AACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA390.1259364505295789No Hit
AATGCGCTCTGAACGTACTCTGCGTTGATACCA360.11624903125807284No Hit
ATCAACGCAGAGTGAATGGGCTCTGAACAAAAA350.11301989150090416No Hit
GAGTGAATGGGCTCTGAACGTACAAAAAAAAAA350.11301989150090416No Hit
CTGAACGTACTCTGCGTAAAAAAAAAAAAAAAA340.10979075174373547No Hit
CGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAA340.10979075174373547No Hit
ATCAACGCAGAGTGAATGGGCTCTGAAAAAAAA320.10333247222939809No Hit
TATCTACGCAGAGTGAATGGGCTCTGAACGAAA320.10333247222939809No Hit
GTGAATGGGCTCTGAACGTAAAAAAAAAAAAAA320.10333247222939809No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACTCA200.00848361327.01
ATCTACG356.3146817E-627.02
GTATCAT504.838512E-927.01
TCTACGC356.3146817E-627.03
TATCTAC356.3146817E-627.01
TATCATC504.838512E-927.02
TACGCAG401.7791504E-523.6255
CTACGCA401.7791504E-523.6254
TCTAATG300.002215492522.53
GCGTTGA2950.021.50847427
AACAATG350.00541442419.2857156
ATCATCG701.7151797E-719.2857153
TCGCAGA651.8277751E-618.6923077
ATCGCAG651.8277751E-618.6923076
TCATCGC651.8277751E-618.6923074
CATCGCA651.8277751E-618.6923075
GTATCAA1450.018.6206911
GAAATGC801.3203087E-515.18757
GTAATGC657.3577714E-414.5384617
TGCGTTG4650.013.64516126
Produced by FastQC (version 0.11.2)