FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate6_16.3520000005ef26.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate6_16.3520000005ef26.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31508
Sequences flagged as poor quality0
Sequence length33
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTATGGTGTTCACGTACTCTGCGTTGATACCA3761.19334772121366No Hit
GTGAATGGGCTATGGTGAAAAAAAAAAAAAAAA2840.9013583851720198No Hit
GTGTTCACGTACTCAAAAAAAAAAAAAAAAAAA2060.653802208962803No Hit
GTGAATGGGCTATGGTGTAAAAAAAAAAAAAAA1710.5427193093817443No Hit
ACGCAGAGTGAATGGGCTATGGTGAAAAAAAAA1660.5268503237273073No Hit
TATCAACGCAGAGTGAATGGGCTATGGTGAAAA1570.4982861495493208No Hit
CCGCTATGGTGTTCACGTACTCTGCGTTGATAC1430.4538529897168973No Hit
GTGAATGGGGCTATGGTGAAAAAAAAAAAAAAA1390.4411578011933477No Hit
TATCAACGCAGAGTGAATGGGCTATGGTGTAAA1200.3808556557064872No Hit
GGGCTATGGTGAAAAAAAAAAAAAAAAAAAAAA1130.3586390757902755No Hit
ACGCAGAGTGAATGGGCTATGGTGTAAAAAAAA1060.3364224958740637No Hit
GTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA990.31420591595785197No Hit
GCATTGGATGGATGCCCGGGCATTGAGAAGGAA890.28246794464897806No Hit
GTATCAACGCAGAGTGAATGGGCTATGGTGAAA880.2792941475180907No Hit
GGGCTATGGTGTTCACGTACTCTGCGTTGATAC850.2697727561254285No Hit
GCGCTATGGTGTTCACGTACTCTGCGTTGATAC800.2539037704709915No Hit
GGGCTATGGTGTAAAAAAAAAAAAAAAAAAAAA800.2539037704709915No Hit
GGTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAA760.24120858194744194No Hit
GCTATGGTGAAAAAAAAAAAAAAAAAAAAAAAA730.2316871905547797No Hit
GCTATGGTGTAAAAAAAAAAAAAAAAAAAAAAA720.22851339342389235No Hit
AACGCAGAGTGAATGGGCTATGGTGAAAAAAAA710.22533959629300496No Hit
GAGTGAATGGGCTATGGTGAAAAAAAAAAAAAA700.22216579916211754No Hit
GTGAATGGGCTATGGTAAAAAAAAAAAAAAAAA680.21581820490034279No Hit
CTATGGTGTTCACGTACTCTGCGTTGATACCAC670.21264440776945537No Hit
GTGCTATGGTGTTCACGTACTCTGCGTTGATAC620.19677542211501842No Hit
CTGCTATGGTGTTCACGTACTCTGCGTTGATAC610.19360162498413103No Hit
GTGAATGGGCTATGGAAAAAAAAAAAAAAAAAA580.18408023359146883No Hit
GCTATGGTGTTCACGTACTCAAAAAAAAAAAAA580.18408023359146883No Hit
GGGAGATACCGTCTGTGATCCATGGATCTCCGA580.18408023359146883No Hit
ACGCTATGGTGTTCACGTACTCTGCGTTGATAC570.18090643646058144No Hit
TATCAACGCAGAGTGAATGGGCTATGGTGTTCA560.17773263932969405No Hit
GAATGGGCTATGGTGAAAAAAAAAAAAAAAAAA560.17773263932969405No Hit
TATCTACGCAGAGTGAATGGGCTATGGTGAAAA550.17455884219880666No Hit
GGCTATGGTGAAAAAAAAAAAAAAAAAAAAAAA500.15868985654436968No Hit
TATCAACGCAGAGTGAATGGGGCTATGGTGAAA500.15868985654436968No Hit
GTATCATCGCAGAGTGAATGGGCTATGGTGAAA490.1555160594134823No Hit
GGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA470.1491684651517075No Hit
GAATGGGGCTATGGTGAAAAAAAAAAAAAAAAA460.14599466802082012No Hit
CATCTAAGTAGCTAAAGGAAGGGAAATCAACCG450.1428208708899327No Hit
GGCTATGGTGTTCACGTACTCTGCGTTGATACC450.1428208708899327No Hit
GTGAATGGGCTATGGTGTTCACGTACTCAAAAA450.1428208708899327No Hit
GAGCTATGGTGTTCACGTACTCTGCGTTGATAC440.13964707375904534No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA430.13647327662815792No Hit
AACGCAGAGTGAATGGGCTATGGTGTAAAAAAA430.13647327662815792No Hit
CAGCTATGGTGTTCACGTACTCTGCGTTGATAC430.13647327662815792No Hit
ACGCAGAGTGAATGGGGCTATGGTGAAAAAAAA420.13329947949727053No Hit
GGGCTATGGTGTTCACGTACTCAAAAAAAAAAA410.13012568236638314No Hit
ATGCTATGGTGTTCACGTACTCTGCGTTGATAC410.13012568236638314No Hit
TCGCTATGGTGTTCACGTACTCTGCGTTGATAC410.13012568236638314No Hit
GCCATGGGGAGATACCGTCTGTGATCCATGGAT410.13012568236638314No Hit
GAGTGAATGGGGCTATGGTGAAAAAAAAAAAAA410.13012568236638314No Hit
GTATCAACGCAGAGTGAATGGGCTATGGTGTAA400.12695188523549575No Hit
GTGAATGGGGCTATGGAAAAAAAAAAAAAAAAA390.12377808810460834No Hit
GTGAATGGGGCTATGGTAAAAAAAAAAAAAAAA390.12377808810460834No Hit
GTGAATGGGGCTATGGTGTAAAAAAAAAAAAAA380.12060429097372097No Hit
CGGCTATGGTGTTCACGTACTCTGCGTTGATAC380.12060429097372097No Hit
GAGTGAATGGGCTATGGTGTAAAAAAAAAAAAA380.12060429097372097No Hit
CGCTATGGTGTTCACGTACTCTGCGTTGATACC370.11743049384283356No Hit
GAATGGGCTATGGTGTAAAAAAAAAAAAAAAAA360.11425669671194617No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT360.11425669671194617No Hit
GGTATCAACGCAGAGTGAATGGGCTATGGTGAA350.11108289958105877No Hit
ACGCAGAGTGAATGGGCTATGGAAAAAAAAAAA350.11108289958105877No Hit
ATCAACGCAGAGTGAATGGGCTATGGTGAAAAA350.11108289958105877No Hit
CGTTAGTAGCGGCGAGCGAGAGCGGATTTGGGA340.10790910245017139No Hit
ACGCAGAGTGAATGGGCTATGGTAAAAAAAAAA340.10790910245017139No Hit
CTATGGTGTAAAAAAAAAAAAAAAAAAAAAAAA330.10473530531928399No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAT306.94288E-527.0000021
CAACTCA455.2819814E-827.01
GGCTATT200.00848567727.020
ATCTACG451.6117574E-624.02
TCTACGC451.6117574E-624.03
TACGCAG451.6117574E-624.05
TATCTAC451.6117574E-624.01
CTACGCA451.6117574E-624.04
GTAGAGT300.002216289522.5000024
ACGTAGA300.002216289522.5000022
CGTAGAG300.002216289522.5000023
TAGAGTG300.002216289522.5000025
TGGGTGT300.002216289522.5000026
AACTCAG608.493971E-720.2500022
ACTCAGA608.493971E-720.2500023
TCAGAGT608.493971E-720.2500025
CTCAGAG608.493971E-720.2500024
TATCATC404.879405E-420.252
AACGTAG350.00541634819.2857151
GGGTGTT350.00541634819.2857157
Produced by FastQC (version 0.11.2)