FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate6_15.3520000005ef0c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate6_15.3520000005ef0c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24185
Sequences flagged as poor quality0
Sequence length33
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTGGATGGATGCCCGGGCATTGAGAAGGAA1740.7194542071531941No Hit
CAGCTATGCCAACGTACTCTGCGTTGATACCAC1530.6326235269795328No Hit
GGGAGATACCGTCTGTGATCCATGGATCTCCGA1110.45896216663221No Hit
GCCATGGGGAGATACCGTCTGTGATCCATGGAT1040.43001860657432295No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT910.37626628075253254No Hit
CATCTAAGTAGCTAAAAAAAAAAAAAAAAAAAA800.3307835435187099No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA790.32664874922472603No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA760.3142443663427745No Hit
TGGCTAGTCTGCGCTCTTTGGACTTTGAAAACT740.3059747777548067No Hit
CCCCTCAGCTATGCCAACGTACTCTGCGTTGAT700.2894356005788712No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA690.28530080628488735No Hit
CATCTAAGTAGCTAAAGGAAGGGAAATCAACCG670.2770312176969196No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA640.26462683481496796No Hit
CTCTTTGGACTTTGAAAACTTAGCGAACTGAAA620.2563572462270002No Hit
CGTTAGTAGCGGCGAGCGAGAGCGGATTTGGGA580.2398180690510647No Hit
GTGAATGGGGAATGCATTGGATGGATGCCCGGG570.23568327475708084No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA530.21914409758114536No Hit
TCTGTGATCCATGGATCTCCGATCGGGAAACCG480.19847012611122597No Hit
AGCTAAAGGAAGGGAAATCAACCGAGACCCCGT480.19847012611122597No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA460.19020053752325822No Hit
GGATGGATGCCCGGGCATTGAGAAGGAAGGACG460.19020053752325822No Hit
TCCAAGCTCCGTGGCTAGTCTGCGCTCTTTGGA460.19020053752325822No Hit
GGCGAAAGGCCATGGGGAGATACCGTCTGTGAT440.18193094893529047No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA430.1777961546413066No Hit
ACCGTATCCAAGCTCCGTGGCTAGTCTGCGCTC430.1777961546413066No Hit
CTTTGGACTTTGAAAACTTAGCGAACTGAAACA420.1736613603473227No Hit
CCCTTCAGCTATGCCAACGTACTCTGCGTTGAT420.1736613603473227No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA410.16952656605333885No Hit
GATACCGTCTGTGATCCATGGATCTCCGATCGG410.16952656605333885No Hit
GCTAAAGGAAGGGAAATCAACCGAGACCCCGTT400.16539177175935496No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA380.15712218317138724No Hit
GAGATACCGTCTGTGATCCATGGATCTCCGATC370.15298738887740335No Hit
AGTCTGCGCTCTTTGGACTTTGAAAACTTAGCG350.1447178002894356No Hit
CCCCCTCAGCTATGCCAACGTACTCTGCGTTGA340.14058300599545173No Hit
CCCTCAGCTATGCCAACGTACTCTGCGTTGATA330.13644821170146784No Hit
CCTCTCAGCTATGCCAACGTACTCTGCGTTGAT330.13644821170146784No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA310.1281786231135001No Hit
CCTCAGCTATGCCAACGTACTCTGCGTTGATAC310.1281786231135001No Hit
GGTATCAACGCAGAGTGAATGGGAAGCAGTGGT300.12404382881951624No Hit
GTGAATGGGTCAGCTATGCCAAAAAAAAAAAAA300.12404382881951624No Hit
ACTCTAACTATGCCAACGTACTCAAAAAAAAAA290.11990903452553235No Hit
TATCCAAGCTCCGTGGCTAGTCTGCGCTCTTTG280.11577424023154848No Hit
GGCATTGAGAAGGAAGGACGCTTTCAGAGGCGA270.1116394459375646No Hit
GTTTAAGCATATCAAAAAAAAAAAAAAAAAAAA270.1116394459375646No Hit
GAGAAGGAAGGACGCTTTCAGAGGCGAAAGGCC270.1116394459375646No Hit
GATTTGGGATTTTAAAAAAAAAAAAAAAAAAAA260.10750465164358074No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCAG200.00847276426.9834376
ATGGGAG300.00220939322.4861975
GTTGATA558.928413E-619.66503127
GGGCATT450.001072457817.98895818
GATGGAT500.002172018216.1900637
GGATGGA500.002172018216.1900636
CGCTCAG500.002172018216.1900636
CGGGCAT500.002172018216.19006317
GGCTCAG500.002172018216.1900636
TGGATGC500.002172018216.1900639
CGTTGAT1301.364242E-1015.59966427
TCTGCGT5050.015.2598727
TATCAAC1454.3655746E-1114.8874131
GGATGCC550.00410015114.71823810
GGCATTG550.00410015114.71823819
CCCGGGC550.00410015114.71823815
GATGCCC550.00410015114.71823811
ATGGATG550.00410015114.7182388
TGCCCGG550.00410015114.71823813
CCGGGCA550.00410015114.71823816
Produced by FastQC (version 0.11.2)