FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate6_11.3520000005ee84.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate6_11.3520000005ee84.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40149
Sequences flagged as poor quality0
Sequence length33
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAATGGGACTGAGGCAAAAAAAAAAAAAAAA1660.4134598620139978No Hit
TATCAACGCAGAGTGAATGGGACTGAGGCAAAA1090.27148870457545643No Hit
TATCAACGCAGAGTGAATGGGGCACTTACGTAC1070.2665072604548058No Hit
GCACTTACGTACTCAAAAAAAAAAAAAAAAAAA970.24160003985155298No Hit
GACTGAGGCAAAAAAAAAAAAAAAAAAAAAAAA930.2316371516102518No Hit
GAATGGGACTGAGGCAAAAAAAAAAAAAAAAAA920.22914642954992653No Hit
GAGTGAATGGGACTGAGGCAAAAAAAAAAAAAA920.22914642954992653No Hit
CTTACGTACTCAAAAAAAAAAAAAAAAAAAAAA890.22167426336895066No Hit
GTGAATGGGACTGAGGCACAAAAAAAAAAAAAA830.2067299310069989No Hit
ACGCAGAGTGAATGGGACTGAGGCAAAAAAAAA770.1917855986450472No Hit
GGACTGAGGCAAAAAAAAAAAAAAAAAAAAAAA670.1668783780417943No Hit
GTGAATGGGGCACTTACGTACTCAAAAAAAAAA660.16438765598146904No Hit
GTGAATGGGGACTGAGGCAAAAAAAAAAAAAAA620.15442476774016786No Hit
CTGAGGCACTTACGTACTCTGCGTTGATACCAC560.13948043537821614No Hit
TACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA530.13200826919724026No Hit
GGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAA500.12453610301626443No Hit
TATCAACGCAGAGTGAATGGGACTGAGGCACAA450.11208249271463797No Hit
GGACTGAGGCACAAAAAAAAAAAAAAAAAAAAA440.10959177065431268No Hit
GACTGAGGCACAAAAAAAAAAAAAAAAAAAAAA430.10710104859398739No Hit
CTTACGTACTCTGCGTTGATACAAAAAAAAAAA410.10211960447333683No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCACT306.990515E-527.0000025
CAACTCA306.990515E-527.0000021
AACTCAG306.990515E-527.0000022
ACTCAGA306.990515E-527.0000023
CTCAGAG306.990515E-527.0000024
CCAAACT200.00851338427.05
TAATACT200.00851338427.05
TACGCAG701.0913936E-1125.0714285
CTACGCA651.1459633E-1024.9230794
AATACTG401.7964976E-523.6256
TCTACGC752.7284841E-1123.43
ATCTACG702.910383E-1023.1428592
GTATCAT351.9917218E-423.1428591
CATCACT351.9917218E-423.1428595
TATCTAC702.910383E-1023.1428591
TCAGAGT351.9917218E-423.1428595
ATCACTG504.0727828E-621.66
CCAACTG454.4620756E-521.06
GACACTG454.4620756E-521.06
AGCACTG454.4620756E-521.06
Produced by FastQC (version 0.11.2)