FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate6_10.3520000005ee6a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate6_10.3520000005ee6a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23825
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCAGAGACGTACGTACTCTGCGTTGATACCA3511.4732423924449107No Hit
CGTACGTACTCAAAAAAAAAAAAAAAAAAAAAA2521.0577124868835257No Hit
GACGTACGTACTCAAAAAAAAAAAAAAAAAAAA1630.6841552990556139No Hit
GCAGAGACGTACGTACTCTGCGTTGATACCACT1430.6002098635886675No Hit
TATCAACGCAGAGTGAATGGGGAGACGTACGTA1240.5204616998950682No Hit
GTGAATGGGGAGACGTACGTACTCAAAAAAAAA1230.5162644281217208No Hit
GAGACGTACGTACTCAAAAAAAAAAAAAAAAAA990.4155299055613851No Hit
TACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA620.2602308499475341No Hit
GTGAATGGGAGAGACGTACGTACTCAAAAAAAA570.23924449108079748No Hit
ACGCAGAGTGAATGGGGAGACGTACGTACTCAA540.22665267576075554No Hit
GTATCAACGCAGAGTGAATGGGGAGACGTACGT540.22665267576075554No Hit
TATCAACGCAGAGTGAATGGGAGAGACGTACGT530.2224554039874082No Hit
GAGTGAATGGGGAGACGTACGTACTCAAAAAAA460.1930745015739769No Hit
AACGCAGAGTGAATGGGGAGACGTACGTACTCA370.155299055613851No Hit
GTGAATGGGATGCAGAGACAAAAAAAAAAAAAA350.14690451206715635No Hit
GTGAATGGGAGACGTACGTACTCAAAAAAAAAA350.14690451206715635No Hit
GGAGAGACGTACGTACTCAAAAAAAAAAAAAAA330.1385099685204617No Hit
TATCAACGCAGAGTGAATGGGATGCAGAGACAA320.13431269674711438No Hit
TATCAACGCAGAGTGAATGGGATGCAGAGACGT310.13011542497376705No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA290.1217208814270724No Hit
GTATCAACGCAGAGTGAATGGGATGCAGAGACG280.11752360965372508No Hit
GTGAATGGGAATGCAGAGACAAAAAAAAAAAAA270.11332633788037777No Hit
CCCCCATGCAGAGACGTACGTACTCTGCGTTGA270.11332633788037777No Hit
ATGCAGAGACGTACAAAAAAAAAAAAAAAAAAA270.11332633788037777No Hit
CGTACGTACTCTAAAAAAAAAAAAAAAAAAAAA270.11332633788037777No Hit
ATGCAGAGACGAAAAAAAAAAAAAAAAAAAAAA260.10912906610703044No Hit
TATCAACGCAGAGTGAATGGGAGACGTACGTAC250.1049317943336831No Hit
GTGAATGGGATGCAGAGAAAAAAAAAAAAAAAA240.10073452256033578No Hit
GAATGGGGAGACGTACGTACTCAAAAAAAAAAA240.10073452256033578No Hit
AGACGTACGTACTCAAAAAAAAAAAAAAAAAAA240.10073452256033578No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCAAC1500.020.6999991
CAACGCA1500.018.94
TCAACGC1600.018.56253
ATCAACG1600.018.56252
AACGCAG1650.017.1818185
ACGCAGA2200.012.8863646
CAGAGTG2250.012.69
GTATCAA750.00214152312.5999991
GCAGAGT2150.012.558148
AGAGTGA2300.012.32608710
CGCAGAG2251.8189894E-1212.07
TGCGTTG2151.5097612E-1011.30232527
GAGTGAA2801.8189894E-1210.60714311
AGTGAAT2851.8189894E-1210.42105212
GCGTTGA1301.02033504E-410.38461527
CTGCGTT2357.185008E-1010.34042626
TCTGCGT3650.09.61643927
CTCTGCG3900.09.026
ACTCTGC4401.8189894E-127.97727325
TGAATGG3652.406523E-97.767123714
Produced by FastQC (version 0.11.2)