FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate5_87.3520000005ec0f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate5_87.3520000005ec0f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20884
Sequences flagged as poor quality0
Sequence length33
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCC1320.6320628232139437No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1140.5458724382302241No Hit
GTGAATGGGAGCTGACGAAAAAAAAAAAAAAAA930.44531698908255124No Hit
CTGACGAAGTACGTACTCTGCGTTGATACCACT670.32081976632828957No Hit
GCTGACGAAGTACGTACTCTGCGTTGATACCAC660.31603141160697185No Hit
AGCTGACGAAGTACGTACTCTGCGTTGATACCA590.28251292855774757No Hit
GGCTAAATACGGGCGAGAGACCGATAGCGAACA580.2777245738364298No Hit
AACAAGTACCGCGAGGTAAAAAAAAAAAAAAAA560.2681478643937943No Hit
GTTTGGGAATGCAGCCCCAATCGGGCGGTAAAT540.2585711549511588No Hit
GCTAAATACGGGCGAGAGACCGATAGCGAACAA510.2442060907872055No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA460.22026431718061676No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA450.21547596245929898No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA450.21547596245929898No Hit
GGCGAGAGACCGATAGCGAACAAGTACCGCGAG450.21547596245929898No Hit
GCCCCAATCGGGCGGTAAATTCCGTCCAAGGCT450.21547596245929898No Hit
GGTAAATTCCGTCCAAGGCTAAATACGGGCGAG410.19632254357402795No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA410.19632254357402795No Hit
GACCGATAGCGAACAAGTACCGCGAGGTAAAAA390.18674583413139245No Hit
ATACGGGCGAGAGACCGATAGCGAACAAGTACC360.1723807699674392No Hit
GAGTGAATGGGAGCTGACGAAAAAAAAAAAAAA360.1723807699674392No Hit
ACGCAGAGTGAATGGGAGCTGACGAAAAAAAAA350.16759241524612142No Hit
AAATACGGGCGAGAGACCGATAGCGAACAAGTA340.16280406052480367No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT320.15322735108216817No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGC320.15322735108216817No Hit
CAAGTACCGCGAGGTAAAAAAAAAAAAAAAAAA310.14843899636085042No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA300.14365064163953264No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA300.14365064163953264No Hit
GTCCAAGGCTAAATACGGGCGAGAGACCGATAG290.1388622869182149No Hit
GTGTAGATCTCGGTGGTCGCCGTATCATTAAAA280.13407393219689714Illumina Single End PCR Primer 1 (100% over 29bp)
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA280.13407393219689714No Hit
GAGCTGACGAAGTACGTACTCTGCGTTGATACC270.1292855774755794No Hit
CAGCTGACGAAGTACGTACTCTGCGTTGATACC270.1292855774755794No Hit
GTGAATGGGAGCTGACAAAAAAAAAAAAAAAAA260.12449722275426163No Hit
GTGAATGGGGCTGACGAAAAAAAAAAAAAAAAA260.12449722275426163No Hit
GTGAATGGGCTGACGAAAAAAAAAAAAAAAAAA250.11970886803294388No Hit
ATGCAGCCCCAATCGGGCGGTAAATTCCGTCCA250.11970886803294388No Hit
TATCAACGCAGAGTGAATGGGGCTGACGAAAAA230.11013215859030838No Hit
TATCAACGCAGAGTGAATGGGAGCTGACGAAGT230.11013215859030838No Hit
GTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA230.11013215859030838No Hit
TGCCCGGACCCTGTCGCACCACGAGGCGCTGTC230.11013215859030838No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCT230.11013215859030838No Hit
CTGTCGCACCACGAGGCGCTGTCTACGAGTCGG220.10534380386899062No Hit
CCCCAGGTCAGGCGGGATTACCCGCTGAGTTTA210.10055544914767285No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA851.263892E-615.8823521
TGCGTTG1503.6379788E-1215.29999927
TAGCTGA600.00722947113.58
TATCAAC802.2159283E-413.52
AATGGGG1204.4559223E-511.254
ATCAACG1050.002177594510.2857143
TCTGCGT3700.09.85135127
TCAACGC1100.00320423959.8181824
CAACGCA1100.00320423959.8181825
CTGCGTT2401.10812834E-89.562526
GTGAATG3109.094947E-119.1451611
TGAATGG3109.094947E-119.1451612
GAATGGG3354.2200554E-108.4626873
CTCTGCG4251.8189894E-128.25882326
GCAGAGT1802.7926418E-48.253
AGTACGT5100.07.94117718
ACTCTGC4453.6379788E-127.887640525
AAGTACG4850.07.793814717
CAGAGTG1750.00196890677.7142864
TACTCTG4607.2759576E-127.630434524
Produced by FastQC (version 0.11.2)