FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate5_80.3520000005eb1d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate5_80.3520000005eb1d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50230
Sequences flagged as poor quality0
Sequence length33
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTGAATGGGCTAACTATCCAC8651.722078439179773No Hit
GTGAATGGGCTAACTATCCACAAAAAAAAAAAA8251.6424447541309974No Hit
CTATCCACGTACTCAAAAAAAAAAAAAAAAAAA7001.3935894883535735No Hit
ACGCAGAGTGAATGGGCTAACTATCCACAAAAA5971.1885327493529763No Hit
GTATCAACGCAGAGTGAATGGGCTAACTATCCA5421.0790364324109096No Hit
GGCTAACTATCCACGTACTCTGCGTTGATACCA4070.810272745371292No Hit
GGCTAACTATCCACAAAAAAAAAAAAAAAAAAA3860.7684650607206849No Hit
GAGTGAATGGGCTAACTATCCACAAAAAAAAAA3680.7326299024487358No Hit
GTGAATGGGCTAACTATCCACGTACTCAAAAAA3340.6649412701572766No Hit
ATCCACAAAAAAAAAAAAAAAAAAAAAAAAAAA3260.6490145331475213No Hit
ATCCACGTACTCAAAAAAAAAAAAAAAAAAAAA3090.6151702170017918No Hit
ACGCAGAGTGAATGGGCTAACTATCCACGTACT2950.5872984272347203No Hit
AACGCAGAGTGAATGGGCTAACTATCCACAAAA2800.5574357953414294No Hit
GTGAATGGGCTAACTATCCAAAAAAAAAAAAAA2750.5474815847103325No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2490.49571968942862826No Hit
AACGCAGAGTGAATGGGCTAACTATCCACGTAC2420.4817837945450926No Hit
CTATCCACAAAAAAAAAAAAAAAAAAAAAAAAA2200.437985267768266No Hit
ACTATCCACAAAAAAAAAAAAAAAAAAAAAAAA2140.4260402150109496No Hit
GTGAATGGGCTAACTATCCACGTACAAAAAAAA2050.40812263587497516No Hit
GGTATCAACGCAGAGTGAATGGGCTAACTATCC2010.40015926737009755No Hit
ATCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1990.39617758311765877No Hit
TATCAACGCAGAGTGAATGGGGCTAACTATCCA1980.3941867409914394No Hit
GTGAATGGGGCTAACTATCCACAAAAAAAAAAA1930.38423253036034244No Hit
ACGCAGAGTGAATGGGCTAACTATCCAAAAAAA1920.382241688234123No Hit
GCTAACTATCCACGTACTCTGCGTTGATACCAC1640.3264981086999801No Hit
CTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1550.3085805295640056No Hit
ATCAACGCAGAGTGAATGGGCTAACTATCCACA1540.3065896874377862No Hit
CTATCCACGTACAAAAAAAAAAAAAAAAAAAAA1520.30260800318534736No Hit
ATCAACGCAGAGTGAATGGGCTAACTATCCACG1510.300617161059128No Hit
GAGTGAATGGGGCTAACTATCCACAAAAAAAAA1500.2986263189329086No Hit
GTGAATGGGGCTAACTATCCACGTACTCAAAAA1500.2986263189329086No Hit
GTATCAACGCAGAGTGAATGGGGCTAACTATCC1460.2906629504280311No Hit
GAGTGAATGGGCTAACTATCCACGTACTCAAAA1430.2846904240493729No Hit
TATCAACGCAGAGTGAATGGGCTAACTATCCAA1430.2846904240493729No Hit
GTGAATGGGCTAACTAAAAAAAAAAAAAAAAAA1390.27672705554449534No Hit
GTGAATGGGGCTAACTAAAAAAAAAAAAAAAAA1390.27672705554449534No Hit
ACGCAGAGTGAATGGGCTAACTATCCACGTACA1350.2687636870396178No Hit
GGCTAACTATCCACGTACTCAAAAAAAAAAAAA1300.2588094764085208No Hit
GTGAATGGGCTAACTATAAAAAAAAAAAAAAAA1190.23691021302010748No Hit
GAGTGAATGGGCTAACTATCCAAAAAAAAAAAA1150.22894684451522992No Hit
ACGCAGAGTGAATGGGGCTAACTATCCACGTAC1080.21501094963169423No Hit
CGTACTCAAAAAAAAAAAAAAAAAAAAAAAAAA1070.2130201075054748No Hit
CTATCCAAAAAAAAAAAAAAAAAAAAAAAAAAA1020.20306589687437787No Hit
ATCCACGTACAAAAAAAAAAAAAAAAAAAAAAA1020.20306589687437787No Hit
ACGCAGAGTGAATGGGCTAACTAAAAAAAAAAA1000.1990842126219391No Hit
CAACGCAGAGTGAATGGGCTAACTATCCACAAA940.18713915986462273No Hit
GAGTGAATGGGGCTAACTATCCACGTACTCAAA920.18315747561218396No Hit
GAGTGAATGGGCTAACTATCCACGTACAAAAAA920.18315747561218396No Hit
ACGCAGAGTGAATGGGCTAACTATAAAAAAAAA920.18315747561218396No Hit
GTATCATCGCAGAGTGAATGGGCTAACTATCCA900.17917579135974518No Hit
AACGCAGAGTGAATGGGCTAACTATCCAAAAAA880.17519410710730637No Hit
ACTATCCACGTACTCAAAAAAAAAAAAAAAAAA880.17519410710730637No Hit
GAATGGGCTAACTATCCACAAAAAAAAAAAAAA870.17320326498108699No Hit
CGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAA870.17320326498108699No Hit
CAACGCAGAGTGAATGGGCTAACTATCCACGTA860.1712124228548676No Hit
GGCTAACTATCCACGTACAAAAAAAAAAAAAAA830.16523989647620946No Hit
GGGCTAACTATCCACGTACTCTGCGTTGATACC810.16125821222377065No Hit
TCCACGTACTCAAAAAAAAAAAAAAAAAAAAAA800.15926737009755126No Hit
GAGTGAATGGGGCTAACTAAAAAAAAAAAAAAA800.15926737009755126No Hit
GGGCTAACTATCCACAAAAAAAAAAAAAAAAAA800.15926737009755126No Hit
CGGCTAACTATCCACGTACTCTGCGTTGATACC800.15926737009755126No Hit
GTGAATGGGGCTAACTATCCAAAAAAAAAAAAA790.15727652797133188No Hit
GTGAATGGGCTAACTATCAAAAAAAAAAAAAAA770.1532948437188931No Hit
TATCAACGCAGAGTGAATGGGCTAACTAAAAAA740.14732231734023493No Hit
GAGTGAATGGGCTAACTAAAAAAAAAAAAAAAA740.14732231734023493No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA740.14732231734023493No Hit
GCTAACTATCCACAAAAAAAAAAAAAAAAAAAA730.14533147521401554No Hit
ACGCAGAGTGAATGGGGCTAACTATCCACAAAA730.14533147521401554No Hit
TATCTACGCAGAGTGAATGGGCTAACTATCCAC720.14334063308779615No Hit
AACGCAGAGTGAATGGGGCTAACTATCCACGTA710.14134979096157677No Hit
CTAACTATCCACGTACTCAAAAAAAAAAAAAAA700.13935894883535735No Hit
GTGAATGGGGCTAACTATAAAAAAAAAAAAAAA700.13935894883535735No Hit
TATCAACGCAGAGTGAATGGGGCTAACTAAAAA700.13935894883535735No Hit
GTGAATGGGGCTAACTATCCACGTACAAAAAAA680.13537726458291857No Hit
TATCAACGCAGAGTGAATGGGCTAACTATAAAA680.13537726458291857No Hit
CTAACTATCCACAAAAAAAAAAAAAAAAAAAAA660.1313955803304798No Hit
GGCTAACTATCCAAAAAAAAAAAAAAAAAAAAA640.12741389607804102No Hit
ATCAACGCAGAGTGAATGGGGCTAACTATCCAC640.12741389607804102No Hit
GTGAATGGGGCTAACAAAAAAAAAAAAAAAAAA630.12542305395182163No Hit
CTAACTATCCACGTACTCTGCGTTGATACCACT630.12542305395182163No Hit
GAATGGGGCTAACTATCCACAAAAAAAAAAAAA620.12343221182560223No Hit
ACGCAGAGTGAATGGGGCTAACTAAAAAAAAAA610.12144136969938285No Hit
AACTATCCACGTACAAAAAAAAAAAAAAAAAAA610.12144136969938285No Hit
GCTAACTATCCACGTACTCAAAAAAAAAAAAAA590.11745968544694405No Hit
ACGCAGAGTGAATGGGCTAACTATCAAAAAAAA590.11745968544694405No Hit
GAGTGAATGGGGCTAACAAAAAAAAAAAAAAAA570.11347800119450527No Hit
CTAACTATCCACGTACAAAAAAAAAAAAAAAAA570.11347800119450527No Hit
GAGTGAATGGGGCTAACTATCCAAAAAAAAAAA560.1114871590682859No Hit
AACTATCCACGTACTCAAAAAAAAAAAAAAAAA550.1094963169420665No Hit
TCAACGCAGAGTGAATGGGCTAACTATCCACAA530.1055146326896277No Hit
CTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA530.1055146326896277No Hit
TCAACGCAGAGTGAATGGGCTAACTATCCACGT520.10352379056340832No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA510.10153294843718894No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACGC356.431881E-626.9930233
TACGCAG356.431881E-626.9930235
CTACGCA356.431881E-626.9930234
CGCTGAG200.00854422626.993022
TATCTAC401.7978893E-523.6424641
GTAGAGT300.002238134422.4941844
ACGTAGA300.002238134422.4941842
CGTAGAG300.002238134422.4941843
TAGAGTG300.002238134422.4941845
ATCTACG454.499936E-520.9945722
AACGTAG350.005437579519.299971
GTATCAA2550.016.95371
GGTATCA952.466768E-715.6431341
TGCGTTG3500.015.05397927
TATCAAC5000.014.8609781
GATACCA600.007383240413.5099827
ATCAACG5950.012.0221012
TCAACGC6000.011.9219173
CAACGCA6400.011.1767974
TCTGCGT8400.010.93665127
Produced by FastQC (version 0.11.2)